Scientia Agricultura Sinica ›› 2024, Vol. 57 ›› Issue (10): 1900-1914.doi: 10.3864/j.issn.0578-1752.2024.10.005

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Response of Maize Growth and Yield with Different Nitrogen Application Rates to Intercropped Leguminous Green Manure

DONG YongJie(), ZHANG Diaoliang, LI Yue, PENG JianChen, HU FaLong, YIN Wen, CHAI Qiang, FAN ZhiLong()   

  1. College of Agronomy, Gansu Agricultural University/Gansu Provincial Key Laboratory of Arid Land Crop Science, Lanzhou 730070
  • Received:2023-11-22 Accepted:2024-03-05 Online:2024-05-16 Published:2024-05-23
  • Contact: FAN ZhiLong

Abstract:

【Objective】 This study aimed to clarify the response of maize growth and yield with different nitrogen application rates to intercropped green manure, so as to provide the basis for establishing maize production pattern with nitrogen fertilizer saving via green manure in arid irrigation area.【Method】 A split plot experiment design was adopted, and two planting patterns of maize intercropping with common vetch (MV) and sole maize (SM) were set up in the main plot. Five nitrogen application rates were set up in the split plot: the recommended nitrogen application rates were N360: 360 kg N·hm-2, application rates reduced with 25% (N270): 270 kg N·hm-2, and that reduced with 50% (N180): 180 kg N·hm-2, reduced with 75% (N90): 90 kg N·hm-2, and no nitrogen application (N0). Leaf area index (LAI), daily leaf volume (LAD), light energy utilization rate, dry matter accumulation and yield of maize were investigated.【Result】 In 75-150 days after maize emergence, the LAI, total LAD and light use efficiency of maize with reduced nitrogen application rate (N270, N180, N90, N0) were increased by intercropped green manure. The maize LAI, total LAD and light use efficiency under M||V with N270, N180, N90, and N0 were significantly increased by 9.8%, 8.2% and 4.6% than that under SM, respectively. The maize LAI, total LAD and light use efficiency under M||V with N270 was not different with that with N360, in 75-150 days after maize emergence, and which was greater than that of other treatments. Somehow, the maize LAI, total LAD and light use efficiency of SM was decreased with reduced nitrogen application rate. The dry matter accumulation of maize under N270, N180, N90, and N0 was significantly increased by intercropped with green manure than that of sole maize, in 90-150 d days after maize emergence, which was increased by 4.6%. In 75-130 days after maize emergence, the growth rate of maize under M||V was significantly increased by 5.9% compared that with SM. With the same reduced nitrogen application rate (N270, N180, N90, and N0), the maize grain yield under M||V was significantly increased by 8.5% than that under SM. The grain yield of intercropped maize under N270 was significantly higher than that of sole maize under N360. Compared with SM, the kernel number per ear and 1000-grain weight of maize under M||V were significantly increased by 6.0% and 6.3%, respectively. The results of the fitting curve showed that the greatest grain yield of maize intercropped with green manure was 14 876.4 kg·hm-2 with nitrogen application rate 261.4 kg·hm-2. While the greatest grain yield of sole maize was 14 012.5 kg·hm-2 with nitrogen application rate of 348.6 kg·hm-2. The effect of reducing nitrogen application on the fresh yield and nitrogen accumulation of green manure was significant. The fresh yield of green manure under M||VN270 was significantly higher than that under M||VN360, M||VN90 and M||VN0 by 3.8%, 4.6%, and 9.7%, respectively, while the nitrogen accumulation with M||VN 270 was significantly higher than that with M||VN90 and M||VN0 by 5.3% and 11.9 %, respectively. The total dry matter accumulation of maize with reduced nitrogen applied rate was increased by intercropped green manure, and then the number of grains per ear and thousand grains weight of maize was increased. Therefore, the intercropped maize could obtain a greater level of grain yield with the condition of reduced nitrogen application rate.【Conclusion】 The treatment of 25% reduced nitrogen rate with intercropped green manure could make maize obtain higher yield than sole maize with the recommended nitrogen application rate. This pattern could be used as a recommended production method for chemical nitrogen fertilizer reduced application of maize in the research region and even similar ecological region.

Key words: green manure intercropping, nitrogen reduced application rate, yield, light use efficiency, maize, common vetch

Fig. 1

Schematic diagram of crop planting in monoculture and intercropping system"

Fig. 2

Leaf area index (LAI) of maize with different treatments M||V represents maize intercropping green manure, and SM represents monoculture maize; N360, N270, N180, N90 and N0 were 360, 270, 180, 90, 0 kg·N·hm-2, respectively. **, *, and NS represent P<0.01, P<0.05, and no significant effect, respectively. The same as below"

Fig. 3

Leaf area duration of maize with different treatments Different lowercase letters indicate significant differences among different treatments at 0.05 probability level. The same as below"

Fig. 4

Light efficiency of maize with different treatments"

Fig. 5

Dynamics of aboveground dry matter accumulation of maize with different treatments"

Fig. 6

Growth rate of maize with different treatments"

Table 1

Grain yield and yield components of maize with different treatments"

年份
Year
种植模式
Cropping pattern
施氮水平
N level
籽粒产量
Grain yield (kg·hm-2)
单位面积穗数
Spike number (spikes·hm-2)
穗粒数
Kernel number per ear
千粒重
1000-grain weight (g)
2022 M||V N360 14232b 82490a 627ab 398a
N270 14823a 82371a 628a 392abc
N180 13981bc 80996abc 599bc 372bc
N90 13340de 80387bcd 578de 358d
N0 11034g 79066de 556fg 336e
SM N360 14281b 82656a 626a 390ab
N270 13656cd 82039ab 590cd 372c
N180 13045e 81025abc 568ef 354d
N90 12081f 79900cde 543g 331e
N0 9827h 78282e 517h 307f
2023 M||V N360 14411b 80643ab 614ab 414a
N270 15046a 81192a 624a 427a
N180 14259bc 80270ab 605b 408a
N90 13537d 79236bcd 571c 364bc
N0 11868f 77934de 559c 347bc
SM N360 14498b 80472ab 616ab 420a
N270 13944c 80531ab 601b 402a
N180 13463d 79865abc 570c 367b
N90 12405e 78756cd 540d 351bc
N0 10475g 77161e 511e 337c
显著性(P值)Significance (P value)
种植模式 Cropping pattern, C 0.000 0.080 0.000 0.000
施氮水平 N fertilizer level, N 0.000 0.000 0.000 0.000
种植模式×施氮水平 C×N 0.000 0.925 0.000 0.024

Fig. 7

Relationships of nitrogen application rate with maize grain yield"

Table 2

The fresh yield and nitrogen accumulation of green manure with different treatments in 2023"

处理
Treatment
绿肥鲜草产量
Fresh yield of green manure (kg·hm-2)
绿肥氮素还田量
Nitrogen accumulation of green manure (kg·hm-2)
M||V N360 16623bc 134ab
M||V N270 17250a 137a
M||V N180 17008ab 136a
M||V N90 16498c 130b
M||V N0 15716d 123c

Table 3

Correlation coefficient and path coefficient of maize between grain yield and yield components, growth index"

指标
Index
简单相关系数
Correlation coefficient
直接通径系数
Direct path coefficient
间接通径系数 Indirect path coefficient
X1 X2 X3 X4 X5 X6
X1 0.867** 0.201 - 0.340 -0.129 -0.232 0.581 0.107
X2 0.939** 0.420 0.163 - -0.137 -0.258 0.633 0.119
X3 0.925** -0.146 0.177 0.395 - -0.258 0.635 0.121
X4 0.942** -0.267 0.174 0.406 -0.141 - 0.648 0.122
X5 0.961** 0.665 0.193 0.400 -0.139 -0.260 - 0.120
X6 0.893** 0.133 0.179 0.376 -0.133 -0.245 0.601 -
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