麦角硫因抑制双孢蘑菇褐变及其与能量代谢关系

doi:10.3864/j.issn.0578-1752.2018.08.014

Abstract

Abstract: 【Objective】How to control and reduce the degree of browning in the process of transport and storage is the focus of Agaricus bisporus research work. The objectives of this research were to study the effect of ergothioneine on postharvest browning of Agaricus bisporus, and further investigate the relevance between browning and energy metabolism, so as to provide scientific basis and production guidance for browning control and shelf life extension. 【Method】A representative Agaricus bisporus cultivar AS2796 was chosen as the experimental material. The harvested mushroom was sprayed with 0.12 mmol?L^-1 ergothioneine. The changes of whiteness, browning, MDA content, adenosine triphosphate (ATP) content, adenosine diphosphate (ADP) content, adenosine monophosphate (AMP) content, energy charge and the activities of succinate dehydrogenase (SDH), cytochrome oxidase (CCO), H⁺-ATPase and Ca²⁺-ATPase of treated samples were determined during storage at 4℃ for 17 days. 【Result】Internal organization browning and MDA content of Agaricus bisporus increased gradually, whereas the whiteness decreased during storage. As compared with the control group, ergothioneine treatment delayed the increase of MDA content and browning degree, effectively reduced the bacteria browning and internal organization browning of Agaricus bisporus. Also, whiteness value of cap of treated Agaricus bisporus was higher than the control group over time. The content of ATP was at a high level, and the lowest point (storage day 17) was also above 0.6 mg?g^-1. Among them, the ATP content of Agaricus bisporus was increased in the early stage of storage, and decreased in the later stage. During the whole storage period, ADP and AMP content both showed a general increasing trend, the ATP and AMP content of treated Agaricus bisporus were distinctly higher than that of the control (P＜0.05), while the ADP content was lower than the control group. The energy charge showed a decreasing trend, and the treated samples were slightly higher than that of the control. Results demonstrated that ergothioneine treatments delayed the decline of ATP content and enhanced the utilization level of ATP in cells of Agaricus bisporus. The activities of SDH, H⁺-ATPase and Ca²⁺-ATPase were obviously higher than that of control during the whole storage (P＜0.05). The activity of CCO at the end (day 17) was higher than that of the control, and was lower than the control in the middle of storage (day 9, day 13). Compared with the control group, ergothioneine treatments inhibited the decrease of the activities of SDH, H⁺-ATPase, and Ca²⁺-ATPase significantly during the storage, which maintained better mitochondrial function. 【Conclusion】All the results indicated that Ergothioneine treatments effectively reduced the postharvest Agaricus bisporus browning, it had a closely relationship with energy metabolism during storage.

Key words: ergothioneine, Agaricus bisporus, browning, energy metabolism

LIN KaiLi, HUANG Qi, HUANG QiHui, JIN ZhenLiang, JIANG TianJia, ZHENG XiaoLin. Browning Inhibition and Energy Metabolism Mechanism of Agaricus bisporus by Ergothioneine Treatment[J].Scientia Agricultura Sinica, 2018, 51(8): 1568-1576.

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