Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (24): 5073-5080.doi: 10.3864/j.issn.0578-1752.2020.24.010

• HORTICULTURE • Previous Articles     Next Articles

Relationship Between LAC Gene Expression and Core Browning of Yali Pear

WANG ZiYu1(),ZHANG YinYin2,LI YueYuan3,LI Ling1,4,YOU LingLing1,4,LI XiaoYan1,4,JIN Zhao5,YAN ShiJie1,4()   

  1. 1College of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384
    2College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384
    3College of Food Science and Engineering, Shanxi Agricultural University, Jinzhong 030801, Shanxi
    4Tianjin Engineering and Technology Research Center of Agricultural Products Processing,, Tianjin 300384
    5Tianjin CNRO Science & Technology CO. ,Ltd., Tianjin 300409
  • Received:2020-02-29 Accepted:2020-10-30 Online:2020-12-16 Published:2020-12-28
  • Contact: ShiJie YAN;


【Objective】 The aim of this study was to explore the relationship between LAC gene expression patterns and core browning of Yali pear under different maturity and cooling storage methods, so as to provide a theoretical basis for further analysis of the mechanism of Yali core browning. 【Method】 Using Yali pear as the material, the Yali pears of different maturity levels (early harvesting, middle harvesting and late harvesting) were treated with rapid cooling and slow cooling to observe the core of the pear during storage. For browning situation, the activity of LAC and the relative expression of LAC gene were determined, and the role of LAC gene in the browning process of Yali pear core was studied. 【Result】 When the Yali pear samples were refrigerated for 60 days, the late-harvested pears browned, and the browning index of late-harvested pears gradually decreased was 0.32, which was 2.56 times the rapid temperature drop during the same period; after 90 days of storage, the middle-harvested browning index decreased slowly, and the index was 0.24, and the browning index of the medium mining was only 0.01. During the storage period, the LAC activity of each treatment group increased gradually and then decreased. The late harvested fruits showed a peak activity and browning occurred after 60 days of storage. The peak of LAC activity of early harvesting and middle harvesting Yali pears appeared in 90 days, and the degree of browning was lower than that of late harvesting Yali. During the storage period, the activity of slow cooling LAC was higher than that of rapid cooling. The LAC14 and LAC7 gene expressions of Yali pears increased first and then decreased. LAC6 realized a change trend of first decline, then increased and then decreased; the expression of LAC14 and LAC7 genes reached the highest when the middle and late harvested pears were stored for 60 days. 【Conclusion】 Compared with slow cooling, rapid cooling treatment reduced the browning of Yali fruit core. During the whole storage period, the activity of LAC showed a trend of rising first, then falling, and then rising again, and the order of activity at its peak was: late harvesting>medium harvesting>early harvesting, which was consistent with the browning trend of the core. LAC was in the Yali fruit Up-regulated expression during heart browning. The rapid cooling was slower than the slow cooling, and the expression of LAC7, LAC14 and LAC6 genes was lower. A timely harvesting with the rapid cooling could inhibit the up-regulated expression of LAC and reduce the browning of Yali pear core.

Key words: Yali pear, core browning, cooling method, laccase, LAC gene

Tab. 1

Primers for real-time q-PCR analysis"

Gene name
Gene ID
Primer sequence (5'-3')
Fragment size (bp)

Fig. 1

Different treatments on browning index of core in Yali pear * indicate significant difference (P<0.05), ** indicate extremely significantsignificant difference (P<0.05)"

Fig. 2

Effects of LAC activity in different treatments of core in Yali pear"

Fig. 5

LAC expression patterns in response to different treatments in core of Yali pear Different lowercase letters indicate significant difference (P<0.05)"

[1] 韩艳文, 廉双秋, 韩云云, 池明, 闫师杰. 鸭梨POD基因的原核表达及成熟度和降温方法对其表达量的影响. 食品科学, 2017,38(9):40-45.
doi: 10.1111/jfds.1973.38.issue-1
HAN Y W, LIAN S Q, HAN Y Y, CHI M, YAN S J. Prokaryotic expression of POD gene from ‘Yali’ pears and effects of maturity and cooling rate on the expression of POD gene during storage. Food Science, 2017,38(9):40-45. (in Chinese)
doi: 10.1111/jfds.1973.38.issue-1
[2] 闫洪波, 程玉豆, 何近刚, 葛文雅, 杨坤, 关军锋. 鸭梨PAL克隆及其在果实发育和机械伤害过程中的表达. 中国农业科学, 2014,47(21):4341-4348.
doi: 10.3864/j.issn.0578-1752.2014.21.019
YAN H B, CHENG Y D, HE J G, GE W Y, YANG K, GUAN J F. Cloning of PAL gene in ‘Yali’ pear and its expression during fruit development and wounding. Scientia Agricultura Sinica, 2014,47(21):4341-4348. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2014.21.019
[3] 闫师杰, 陈计峦, 梁丽雅, 汪政富, 胡小松. 降温方法对不同采收成熟度鸭梨某些生理指标的影响. 中国食品学报, 2008,8(4):96-101.
YAN S J, CHEN J L, LIANG L Y, WANG Z F, HU X S. Effects of different cooling methods on some physiological indexes of different maturity Yali pear after harvest. Journal of Chinese Institute of Food Science and Technology, 2008,8(4):96-101. (in Chinese)
[4] 吴耕西, 周宏伟, 汪建民. 鸭梨酶促褐变的生化机制及底物鉴定. 园艺学报, 1992,19(3):198-202 .
WU G X, ZHOU H W, WANG J M. Biochemical mechanism and substrate identification of enzymatic browning of ‘Yali’ pears. Acta Horticulturae Sinica, 1992,19(3):198-202. (in Chinese)
[5] 闫师杰, 何爱红, 胡小松. 鸭梨果实采后逆境伤害发生机理的研究进展. 保鲜与加工, 2005,5(4):3-6.
YAN S J, HE A H, HU X S. Advances on mechanism of stress injury of postharvest Chinese Yali pear fruit. Storage and Process, 2005,5(4):3-6. (in Chinese)
[6] 霍君生, 佟代言, 刘彩莉, 史吉平. 鸭梨果心褐变过程中膜脂过氧化及细胞内膜粘度的变化. 园艺学报, 1995,22(3):221-224.
HUO J S, TONG D Y, LIU C L, SHI J P. Changes of superoxidation and endomembrane microviscosity of the core of Ya pears during browning development. Acta Horticulturae Sinica, 1995,22(3):21-224. (in Chinese)
[7] 王静. 细胞区室化功能破坏导致的果蔬酶促褐变机理及控制方法. 陕西农业科学, 2014,60(8):62-65.
WANG J. Enzymatic browning mechanism and control method of fruit and vegetable caused by cell compartmentalization. Shaanxi Journal of Agricultural Sciences, 2014,60(8):62-65. (in Chinese)
[8] 周向军, 杨金龙, 路宛如. 莴笋多酚氧化酶、过氧化物酶的特性及抑制作用研究. 食品工业科技, 2015,36(5):166-174.
ZHOU X J, YANG J L, LU W R. Properties and inhibitions of polyphenoloxidase and peroxidase from Asparagusplettuce. Science and Technology of Food Industry, 2015,36(5):166-174. (in Chinese)
[9] 何利华. 鸭梨采后多酚氧化酶PPO同工酶及基因与果实褐变关系的研究[D]. 石河子: 石河子大学, 2010.
HE L H. Postharvest pear polyPhenoloxidase (PPO) is ozymes and genetic relationship with the browning of fruit[D]. Shihezi: Shihezi University, 2010. (in Chinese)
[10] 李晓丹, 宋方圆, 朱文嫱, 梁丽雅, 闫师杰. 鸭梨采后果肉LOX同工酶变化及其与果实褐变关系的研究. 华北农学报, 2013,28(4):214-217.
doi: 10.3969/j.issn.1000-7091.2013.04.039
LI X D, SONG F Y, ZHU W Q, LIANG L Y, YAN S J. Study on LOX isozymes changes of Yali pear pulp after harvest and its relationship with fruit browning. Acta Agriculturae Boreali-Sinica, 2013,28(4):214-217. (in Chinese)
doi: 10.3969/j.issn.1000-7091.2013.04.039
[11] 樊晓岚, 李月圆, 张引引, 韩云云, 李玲, 闫师杰, 肖丽霞. 降温方式对鸭梨采后FADLOX基因表达及其褐变的影响. 食品科学, 2019,40(5):222-227.
FAN X L, LI Y Y, ZHANG Y Y, HAN Y Y, LI L, YAN S J, XIAO L X. Effect of cooling method on FAD and LOX gene expression and core browning in postharvest Yali pears. Food Science, 2019,40(5):222-227. (in Chinese)
[12] MANGARAJ S, GOSWAMI T K, PANDA D K. Modeling of gas transmission properties films used for MA packaging of fruits. Journal of Food Science and Technology-Mysore, 2015,52(9):5456-5469.
doi: 10.1007/s13197-014-1682-2
[13] OPARA U L, CALEB O J, UCHECHUKWU-AGUA A D. Evaluating the impacts of selected packaging materials on the quality attributes of cassava flour. Journal of Food Science, 2016,81(2):324-331.
[14] 万云洋, 杜予民. 漆酶结构与催化机理. 化学通报, 2007,70(9):662-670.
WAN Y Y, DU Y M. Structure and catalytic mechanism of Laccases. Chemistry, 2007,70(9):662-670. (in Chinese)
[15] FANG F, ZHANG X L, LUO H H, ZHOU J J, GONG Y H, LI W J, SHI Z W, HE Q, WU Q, LI L, JIANG L L, CAI Z G, OREN-SHAMIR M, ZHANG Z Q, PANG X Q. An intracellular Laccase is responsible for the epicatechin mediated anthocyanin degradation of litchifruit pericarp. Plant Physiology, 2015,169(4):2391-2408.
doi: 10.1104/pp.15.00359 pmid: 26514808
[16] 蒋跃明. 荔枝果实采后果皮褐变的研究[D]. 广州: 中山大学, 1999.
JIANG Y M. Study on the browning of litchi fruits [D]. Guangzhou: Sun Yat-sen University, 1999. (in Chinese)
[17] TURLAPATI P V, KIM K W, DAVIN L B, LEWIS N G. The laccase multigene family in Arabidopsis thaliana: Towards addressing the mystery of their gene function (s). Planta, 2011,233:439-470.
doi: 10.1007/s00425-010-1298-3
[18] LE ROY J, BLERVACQ AS, CREACH A, HUSS B, HAWKINS S, NEUTELINGS G. Spatial regulation of monolignol biosynthesis and Laccase genes control developmental and stress-related lignin in flax. BMC Plant Biology, 2017,17:124.
doi: 10.1186/s12870-017-1072-9 pmid: 28705193
[19] WANG J H, FENG J J, JIA W T, FAN P X, BAO H X, LI S Z, LI Y X. Genome-wide identification of sorghum bicolor laccases reveals potential targets for lignin modification. Frontiers in Plant Science, 2017,8:714.
doi: 10.3389/fpls.2017.00714 pmid: 28529519
[20] LIU Q Q, ZHENG L, HE F, ZHAO F J, SHEN Z G. Transcriptional and physiological analyses identify a regulatory role for hydrogen peroxide in the lignin biosynthesis of copper-stressed rice roots. Plant and Soil, 2014,387:323-336.
doi: 10.1007/s11104-014-2290-7
[21] BRYAN A C, JAWDY S, GUNTER L, GJERSING E, SYKES R, HINCHEE M A W, WINKELER K A, COLLINS C M, ENGLE N, TSCHAPLINSKI T J. Knockdown of a Laccase in Populus deltoides confers altered cell wall chemistry and increased sugar release. Plant Biotechnology Journal, 2016,14:2010-2020.
doi: 10.1111/pbi.12560 pmid: 26997157
[22] YAN S J, LI L, HE L H, LIANG L Y, LI X D. Maturity and cooling rate affects browning, polyphenol oxidase activity and gene expression of ‘Yali’ pears during storage. Postharvest Biology and Technology, 2013,85:39-44.
doi: 10.1016/j.postharvbio.2013.04.016
[23] FLOCH C, ALARCON-GUTIERREZ E, CRIQUET S. ABTS assay of phenol oxidase activity in soil. Journal of Microbiological Methods, 2007,71(3):319-324.
doi: 10.1016/j.mimet.2007.09.020 pmid: 18006094
[24] 闫洪波, 葛文雅, 杨坤, 程玉豆, 关军锋. 鸭梨肌动蛋白2基因克隆和表达分析. 河北农业大学学报, 2012,35(1):27-30.
YAN H B, GE W Y, YANG K, CHENG Y D, GUAN J F. Cloning and expression analysis of Actin2 gene in Yali pear. Journal of Agricultural University of Hebei, 2012,35(1):27-30. (in Chinese)
[25] 贺立红, 宾金华. 高等植物中的多酚氧化酶. 植物生理学通讯, 2001,37(4):340-345.
HE L H, BIN J H. Polyphenol oxidase in higher plants. Plant Physiology Communications, 2001,37(4):340-345. (in Chinese)
[26] 张琥, 王金勇. 红外与漂烫处理下马铃薯多酚氧化酶的失活机理研究. 食品工业, 2017,38(2):9-13.
ZHANG H, WANG J Y. Study on inactivation mechanism of potato PPO under infrared processing and blanching processing. The Food Industry, 2017,38(2):9-13. (in Chinese)
[27] NIU B, GUO L, ZHAO M M, LUO T, ZHANG R, ZHANG F L, HOU P, ZHANG Y, XU Y, WANG S H. Molecular cloning, characterization, and expression of an ω-3 fatty acid desaturase gene from Sapium sebiferum. Journal of Bioscience and Bioengineering, 2008,106(4):375-380.
doi: 10.1263/jbb.106.375 pmid: 19000614
[28] DING C K, CHACHIN K, UEDA Y, IMAHORI Y. Purification and properties of polyphenol oxidase from loquat fruit. Journal of Agricutural and Food Chemistry, 1998,46:4144-4149.
doi: 10.1021/jf980093s
[29] 林河通, 席玙芳, 陈绍军. 果实贮藏期间的酶促褐变. 福州大学学报(自然科学版), 2002,30(Z1):696-703.
LIN H T, XI Y F, CHEN S J. A review of enzymatic browning in fruit during storage. Journal of Fuzhou University (Natural Science Edtion), 2002,30(Z1):696-703. (in Chinese)
[30] 刘保华, 肖茜, 冯超, 孙进华, 王家保. 荔枝漆酶基因LcLAC的克隆与表达分析. 园艺学报, 2012,39(5):853-860.
LIU B H, XIAO Q, FENG C, SUN J H, WANG J B. Cloning and expression analysis of the Laccase gene from Litchi chinensis. Acta Horticulturae Sinica, 2012,39(5):853-860. (in Chinese)
[31] 帅良, 赵昱清, 廖玲燕, 宋幕波, 朱东建, 蔡文, 段振华, 吴振先, 韩冬梅. 龙眼漆酶基因(Dllac)的克隆及表达分析. 食品工业科技, 2017,38(13):95-100.
SHUAI L, ZHAO Y Q, LIAO L Y, SONG M B, ZHU D J, CAI W, DUAN Z H, WU Z X, HAN D M. Cloning and expression analysis of the Laccase gene (Dllac) from Dimocarpus longan. Science and Technology of Food Industry, 2017,38(13):95-100. (in Chinese)
[32] 周一鹏. 柑橘漆酶基因家族的鉴定及抗逆性研究[D]. 扬州: 扬州大学, 2018.
ZHOU Y P. Genome-wide identification and characterization of Laccase gene family and resistance study in Citrus sinensis[D]. Yangzhou: Yangzhou University, 2018. (in Chinese)
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