Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (24): 5073-5080.doi: 10.3864/j.issn.0578-1752.2020.24.010

• HORTICULTURE • Previous Articles     Next Articles

Relationship Between LAC Gene Expression and Core Browning of Yali Pear

WANG ZiYu1(),ZHANG YinYin2,LI YueYuan3,LI Ling1,4,YOU LingLing1,4,LI XiaoYan1,4,JIN Zhao5,YAN ShiJie1,4()   

  1. 1College of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384
    2College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384
    3College of Food Science and Engineering, Shanxi Agricultural University, Jinzhong 030801, Shanxi
    4Tianjin Engineering and Technology Research Center of Agricultural Products Processing,, Tianjin 300384
    5Tianjin CNRO Science & Technology CO. ,Ltd., Tianjin 300409
  • Received:2020-02-29 Accepted:2020-10-30 Online:2020-12-16 Published:2020-12-28
  • Contact: ShiJie YAN E-mail:prince1893@yeah.net;yanshijie@126.com

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Abstract:

【Objective】 The aim of this study was to explore the relationship between LAC gene expression patterns and core browning of Yali pear under different maturity and cooling storage methods, so as to provide a theoretical basis for further analysis of the mechanism of Yali core browning. 【Method】 Using Yali pear as the material, the Yali pears of different maturity levels (early harvesting, middle harvesting and late harvesting) were treated with rapid cooling and slow cooling to observe the core of the pear during storage. For browning situation, the activity of LAC and the relative expression of LAC gene were determined, and the role of LAC gene in the browning process of Yali pear core was studied. 【Result】 When the Yali pear samples were refrigerated for 60 days, the late-harvested pears browned, and the browning index of late-harvested pears gradually decreased was 0.32, which was 2.56 times the rapid temperature drop during the same period; after 90 days of storage, the middle-harvested browning index decreased slowly, and the index was 0.24, and the browning index of the medium mining was only 0.01. During the storage period, the LAC activity of each treatment group increased gradually and then decreased. The late harvested fruits showed a peak activity and browning occurred after 60 days of storage. The peak of LAC activity of early harvesting and middle harvesting Yali pears appeared in 90 days, and the degree of browning was lower than that of late harvesting Yali. During the storage period, the activity of slow cooling LAC was higher than that of rapid cooling. The LAC14 and LAC7 gene expressions of Yali pears increased first and then decreased. LAC6 realized a change trend of first decline, then increased and then decreased; the expression of LAC14 and LAC7 genes reached the highest when the middle and late harvested pears were stored for 60 days. 【Conclusion】 Compared with slow cooling, rapid cooling treatment reduced the browning of Yali fruit core. During the whole storage period, the activity of LAC showed a trend of rising first, then falling, and then rising again, and the order of activity at its peak was: late harvesting>medium harvesting>early harvesting, which was consistent with the browning trend of the core. LAC was in the Yali fruit Up-regulated expression during heart browning. The rapid cooling was slower than the slow cooling, and the expression of LAC7, LAC14 and LAC6 genes was lower. A timely harvesting with the rapid cooling could inhibit the up-regulated expression of LAC and reduce the browning of Yali pear core.

Key words: Yali pear, core browning, cooling method, laccase, LAC gene

Tab. 1

Primers for real-time q-PCR analysis"

基因名称
Gene name		 基因号
Gene ID		 引物序列（5'-3'）
Primer sequence (5'-3')		 扩增片段大小
Fragment size (bp)
pbACT NW_008988356.1 F：TGGTATAGTGAGCAACTGGGATGA
R：CCTCTGTGAGCAGAACTGGGTG		 104
pbLAC6 NW_008988414.1 F：AACACCAAGGACATCGTGACCATC
R：TGACGCCGTGCCAGTGAATTG		 184
pbLAC14 NW_008988132.1 F：CGCAGTTCACACCGAGATCATCC
R：TTGCTAGGCTTCTGAAGTGCTTGG		 85
pbLAC7 NW_008988996.1 F：TTGTTGGGTATGGGAGTG
R：AGGAGGATGAGGAAGGGT		 242

Fig. 1

Different treatments on browning index of core in Yali pear * indicate significant difference (P<0.05), ** indicate extremely significantsignificant difference (P<0.05)"

Fig. 2

Effects of LAC activity in different treatments of core in Yali pear"

Fig. 5

LAC expression patterns in response to different treatments in core of Yali pear Different lowercase letters indicate significant difference (P<0.05)"

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