不同年份冷水胁迫下水稻抽穗期和产量性状的QTL分析

doi:10.3864/j.issn.0578-1752.2016.18.003

Abstract

Abstract: 【Objective】Heading date, seed setting rate, thousand grain weight and grain weight per plant are traits associated with yield and these traits are greatly influenced by cold water. Therefore, the QTL analysis of heading date, seed setting rate, thousand grain weight and grain weight per plant of rice under cold water stress were conducted in the present study in order to provide a scientific basis for the rice genetic mechanism of cold tolerance and molecular marker assisted breeding. 【Method】 Totally 190 recombinant inbred line (RIL) populations derived from two rice varieties (Dongnong 422 and Kongyu131) were used as materials under cold water irrigation at booting stage, a linkage map was constructed using 155 SSR markers. The correlation analysis on the initial heading (IHD) date, full heading (FHD) date, seed setting rate, thousand grain weight and grain weight per plant were conducted to detect (QTL) these traits by stepwise joint QTL mapping method. The correlations between QTLs of different traits were determined by using the hypergeometric probability function. 【Result】 IHD and FHD were postponed under cold water irrigation in two parents and RIL. Seed setting rate, thousand grain weight and grain weight per plant had significant changes. Those traits had a positive or a negative correlation. A total of 71 QTLs were detected by using stepwise joint QTL mapping method, among them, 37 QTL were associated with cold tolerance, among them, 28 were detected under single- environment, 11 QTL explained beyond 10% of phenotypic variance, 10 QTL were detected under the single-treatment, 6 QTL explained beyond 10% of phenotypic variance, all 17 QTL were associated with cold tolerance. Five QTL had significant QE interaction under the Joint QTLs combining data of all environments. Twenty pairs of additive×additive epistasis were detected, and two main-effect QTL had epistatic interaction. Hypergeometric probability function showed that QTL was associated among those traits. By comparing the mapping results, some of the detected QTLs were in the same or adjacent chromosomal regions of previously reported QTLs, which include qIHD7-2, qFHD7-2, qFHD7-1 and qIHD7-1. These four QTL were stable in different environments, meanwhile, qIHD7-1, qIHD7-2, qFHD7-1, qSSR7-1 and qSSR7-2 were associated with cold tolerance. These QTL loci may provide a basis for molecular breeding for cold resistance in rice.【Conclusion】Seed setting rate has similar genetic mechanism with heading date, thousand grain weight and grain weight per plant under cold water stress. However, heading date, thousand grain weight and grain weight per plant are independently inherited. Meanwhile, it is more harder to breed the rice has high seed setting rate than heading date, thousand grain weight and grain weight per plant under cold water stress.

Key words: Japonica rice, cold water stress, heading data, yield traits, QTL

YANG Luo-miao, SUN Jian, ZHAO Hong-wei, WANG Jing-guo, LIU Hua-long, ZOU De-tang. QTL Analysis of Heading Date and Yield Traits in Japonica Rice Under Cold Water Stress in Different Years[J].Scientia Agricultura Sinica, 2016, 49(18): 3489-3503.

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