不同培养基继代培养球孢白僵菌对西花蓟马毒力和产孢量的影响

doi:10.3864/j.issn.0578-1752.2016.15.012

Abstract

Abstract: 【Objective】The objective of this study is to assess the capability of the Beauveria bassiana against Frankliniella occidentalisin the field, and explore the influence of subculture on virulence and conidial production of the B. bassiana, propose an effective method for preventing the retrogression during successive subculture, and provide a basis for the large-scale production technology of B. bassiana. 【Method】 The virulence of 28 fungal strains to adult F. occidentalis was evaluated by soaking method with B. bassiana concentration of 1×10⁷ conidia/mL in the laboratory. The adults were dipped for 5 s in the conidial suspension. Conidial production of the 4 high virulent strains was measured on corn meal agar (CMA) medium and sabouraud dextrose agar yeast (SDAY) medium, screening high conidial production strain and spore production medium by comparing conidial production of different strains on different media. Subsequently, strain DZDC-9 was subcultured on three media (CMA medium, CMA medium with cicada exuviae, and CMA medium with F. occidentalis cadaver powder) to compare its virulence and conidial production over five subcultures. The relationship between conidial production and virulence was analyzed. 【Result】Among 28 strains of entomopathogenic fungi B. bassiana tested for virulence against F. occidentalis in laboratory bioassays, strains DZDC-9, GZGY-5, WLMQ1-8 and SZ-26 were found as the most potent. The corrected cumulative mortalities were over 90% after 5 days, and LT₅₀<3 d. Conidial production obtained from the CMA medium of each strain was higher than from SDAY medium, and strain DZDC-9 had the higher conidial production among the four strains. The results demonstrated that the virulence of strain DZDC-9 declined with successive subculture. However, when the strain was subcultured on the CMA medium with 0.5% cicada exuviae or 0.5% F. occidentalis cadaver powder as an additive, the virulence increased with successive subculture. Conidial production was not significantly different between the same generation subculture on CMA medium with cicada exuviae and CMA medium with F. occidentalis cadaver powder, but they were significantly greater than conidial production obtained from CMA medium. A positive correlation was found between sporulation and virulence of B. bassiana conidia under different media and culture conditions.【Conclusion】The potential of B. bassiana strain DZDC-9 for the control of F. occidentalis was studied, and proposed an effective method for stabilizing the virulence and sporulation of B. bassiana. Conidial production could be used as an indicator for virulence evaluation.

Key words: Frankliniella occidentalis, Beauveria bassiana, subculture, virulence, conidial production

ZHANG Hui, WU Sheng-yong, LI Juan, ZHANG Lu-lu, ZHANG Lin-ya, LEI Zhong-ren. Influence of Subculture on Virulence to Frankliniella occidentalis and Conidial Production of the Entomopathogenic Fungus Beauveria bassiana[J].Scientia Agricultura Sinica, 2016, 49(15): 2977-2987.

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Influence of Subculture on Virulence to Frankliniella occidentalis and Conidial Production of the Entomopathogenic Fungus Beauveria bassiana

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