Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (21): 4209-4218.doi: 10.3864/j.issn.0578-1752.2015.21.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

The Coding Regions Allelic Variations of TaGW2-6A and the Relationship Between the Allele and Drought Tolerance of Wheat (Triticum aestivum L.)

LI Yang, LI Li-qun, GAO Xin, YANG Lu, KOU Cheng, Lü Qian, LIU Tian-hong, DU Deng-feng, LI Xue-jun   

  1. College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology in Arid Areas, Yangling 712100, Shaanxi
  • Received:2015-04-21 Online:2015-11-01 Published:2015-11-01

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Abstract: 【Objective】 Drought is one of the most significant factors that restrict wheat (Triticum aestivum L.) growth and reduce productivity. The kernel size and its stability affect the yield of wheat in different rainfall locations. The research of grain weight and the relationship between the drought resistance has a vital significance to high and stable yield. The aim of this study is to gain further insight into the drought resistance of different allelic variations of kernel weight related gene, determine the allelic gene variant genotype with strong drought resistance and high yield ability, making for marker-assisted selection of drought resistance and high yield varieties and provide a theoretical basis for genetic improvement. 【Method】 An allelic variation with single T-base insertion at 977 bp of TaGW2-6A was detected in Lankaodali with large kernel, designated as TaGW2-6A-T (TT genotype), but was not found in Chinese Spring (tt genotype). According to the sequence of “T” insert, primers were designed to detect the coding regions allelic variations of TaGW2-6A by High Resolution Melting Curve (HRM) analysis technique, and the sequence of PCR products were analyzed. To gain further insight into the role of allelic variations of TaGW2 in drought tolerance, various physiological parameters related to stress response and kernel weight related traits including the chlorophyll fluorescence kinetics parameters, stem soluble sugar content, drought resistance index and kernel weight related traits of 325 recombinant inbred lines (RILs) derived from a cross between Chinese Spring and Lankaodali were analyzed under different water conditions at flowering, filling and milk stages.【Result】 Results showed that it was accurate to detect the wheat coding regions allelic variations of TaGW2-6A by HRM analysis technique, and classified as Lankaodali genotype (TT), Chinese Spring genotype (tt) and heterozygous genotype (Tt). The PCR product sequencing results showed that the solubility curve difference was caused by the “T” base insert. Compared to tt genotype, the kernel length, kernel width and kernel weight of TT/Tt genotype increased significantly. The chlorophyll fluorescence kinetics parameters, drought resistance index and stem soluble sugar content of the two genotypes were significantly or extremely significantly different (P＜0.05 or P＜0.01). The chlorophyll fluorescence kinetics parameters of RILs and their parents reduced, except the initial fluorescence Fo, while the stem soluble sugar content increased under drought stress. The variability of chlorophyll fluorescence parameters of RILs with TT/Tt genotype was smaller than that of RILs with tt genotype. While stem soluble sugar content of RIL with TT genotype was higher than that of RILs with tt genotype in Yangling and Qianxian. The accumulation efficiency and remobilization efficiency of stem soluble sugar content increased with TT/Tt genotype, while decreased with tt genotype. In addition, TT genotype plays an important role to the chlorophyll fluorescence parameters, the stem soluble sugar content and kernel weight. The study revealed that, compared with the RILs with tt genotype, the photosynthetic institutions of RILs with TT genotype damaged smaller, and accumulated more osmotic regulation substances to ensure the grain filling when suffering drought stress. 【Conclusion】 TaGW2-6A variation (TT genotype) was better able to prevent reducing production in different locations with strong drought resistance and stable yield ability than wild-type (tt) plants, and the ability of drought resistance is close to Jinmai 47.

Key words: common wheat, RIL population, TaGW2-6A allelic variation, kernel weight related gene, drought tolerance

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