诱导多能干细胞体外定向分化为雄性生殖细胞研究进展

doi:10.3864/j.issn.0578-1752.2014.16.016

Abstract

Abstract: Induced pluripotent stem cells (iPS cells) refers to reprograming animals or human somatic cells by gene transfer technology, i.e. using so-called packed viral vectors or particles to infect the cells to be induced. Different somatic cells can be induced into iPS cells through different vectors or different transcription factors combination, such as fibroblasts, hepatocytes, keratinocytes and cord blood, etc. As a new member, iPS cells were similar to embryonic stem cells, i.e. clonal morphology, gene expression pattern, surface marker, embryoid bodies formation, differentiative capacity, teratoma and chimeras (in mice) and so on. Just like ESCs, studies have shown that iPS cells can be induced into different cells in vitro under the conditions of specific induction, including myocardial cells, blood cells and germ cells. It shortens the distance between stem cells and clinical disease, Therefore iPS cells have become the most promising seed cells in cell therapy and tissue organ regeneration, and they have a potential value in the cells’ alternative treatment, pathogenesis research and new drug screening simultaneously. Male sterility not only affects human normal life, but is also very adverse to the development of animal husbandry. Great progress has been made in that iPS cells from humans and mice can be differentiated into primordial germ cells (PGCs), spermatozoa and their precursors. These findings can help not only avoid the difficulties in obtaining ESCs, immune rejection and ethical problems, which may be aroused by using embryonic stem cells, but also provide a good research platform for revealing the developmental mechanism of male germ cells. Patients can use their iPS cells derived male gametes to reproduction. Therefore, differentiation of iPS cells into male germ cells brings new hope for the treatment of male sterility in the future. In addition, differentiation of iPS cells in vitro has broad application potentials for modern animal husbandry. It can be used to produce the transgenic animals. Here, the authors highlighted the advances in research of the differentiation of iPS cells into male germ cells in vitro and its potential application prospects from the different culture conditions and inducers and the prospects in application of the differentiation of iPS cells.

Key words: induced pluripotent stem cells, differentiation, spermatogonial stem cells, male germ cells

CLC Number:

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DU Jun-Hui, CAO Wen-Guang. Advances in Differentiation of iPS Cells into Male Germ Cells[J].Scientia Agricultura Sinica, 2014, 47(16): 3308-3314.

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