Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (14): 2897-2906.doi: 10.3864/j.issn.0578-1752.2014.14.020

• RESEARCH NOTES • Previous Articles    

Separation and Purification of Polyphenol in Litchi Pulp by Macroporous Resin

 SU  Dong-Xiao-1, 2 , ZHANG  Rui-Fen-1, ZHANG  Ming-Wei-1, HUANG  Fei-1, 2 , WEI  Zhen-Cheng-1, ZHANG  Yan-1, TI  Hui-Hui-1, DENG  Yuan-Yuan-1, TANG  Xiao-Jun-1   

  1. 1、Sericultural and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/ Key Laboratory of Functional Food, Ministry of Agriculture, Guangzhou 510610;
    2、Department of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070
  • Received:2014-02-28 Online:2014-07-15 Published:2014-04-21

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Abstract: 【Objective】Litchi (Litchi chinenesis Sonn.) is an important subtropical to tropical fruit which contain a high amount of phenolics. Previous studies found that litchi exhibits excellent antioxidant, radioprotective and hepatoprotective activities. The aim of this study is to screen a resin which has a good adsorption and desorption performance of phenols of litchi, and optimize the separation process parameters, thus providing a theoretical basis for establishing polyphenols purification technology and identification of polyphenols construction of litchi. The separation and purification process of litchi pulp polyphenol was established by macroporous resin.【Method】The static adsorption and desorption performance of eleven different polarities macroporous resins (HPD-200A, HPD-400A, HPD-100, HPD-500, HPD-600, HPD-722, HPD-826, D4020, NKA-II, NKA-9 and AB-8) to total phenolics and total flavonoids of fresh litchi pulp chilled acetone/water extracts were compared to select suitable resin for purification of phenolic compounds. Kinetic curves of adsorption were studied. The dynamic adsorption and desorption process parameters, including sample concentration (3.2, 1.6, 0.8 and 0.4 mg•mL-1), flow rate (4.5, 3.0 and 1.5 BV/h), and eluent of ethanol concentration (95%, 80% and 60%), of macroporous resin of HPD-826 were optimized. The phenolic compound types and contents of litchi pulp were analyzed by HPLC with the retention time of standard including catechin, gallic acid, chlorogenic acid, vanillic acid, clove acid, caffeic acid, epicatechin, ferulic acid, 4-methylcatechol, coumarin, rutin, quercetin, resveratrol and quercetin-3-O- rutinose-7-O-rhamnose.【Result】The results showed that HPD-826 macroporous resin exhibited the best capability of adsorption and desorption of total phenolics and total flavonoids in litchi pulp. And the macroporous resin reached equilibrium within 4 h. The optimal separating process parameters were as follows: the concentration of litchi pulp extract and the sampling rate were 0.8 mg/mL and 3.0 BV/h, respectively, and the elution concentration and flow velocity were 95% ethanol and 3.0B V/h, respectively. The contents of phenolic compounds of litchi pulp were deduced by about 15% compared to before purification, but the phenolic profiles of litchi pulp were not changed after adsorption and desorption by HPD-826 macroporous resin. Nine phenolic compounds, 3,4 dihydroxybenzoic acid, catechin, vanillic acid, caffeic acid, syringic acid, epicatechin, quercetin 3-O-rutinoside-7-O-α-L- rhamnosidase, ferulic acid and rutin, were preliminary identified by HPLC. The major phenolic profiles were quercetin 3-O- rutinoside-7-O-α-L-rhamnosidase, rutin and epicatechin. The percentage contribution of the three compounds to the total phenolic content was 94.37%.【Conclusion】In conclusion, HPD-826 macroporous resin could be applied to purify total phenolics and total flavonoids in litchi pulp.

Key words: litchi , total phenolics , total flavonoids , macroporous resin , separation and purification

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