Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (11): 2242-2250.doi: 10.3864/j.issn.0578-1752.2014.11.018

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Effect of Kuqin Compound on mRNA Transcription of Bcl-2/BAX in PK-15 Cells Infected by TGEV

 SHAO  Qiu-Hong, LING  Rong-Bin, ZHU  Zhao-Rong, LIU  Juan, GUO  Zhi-Xing, WANG  Peng   

  1. Traditional Chinese Medicine Innovation Laboratory, Veterinary Science Research Centre of Rongchang Campus, Southwest University, Chongqing 402460
  • Received:2013-09-16 Online:2014-06-06 Published:2014-03-07

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Abstract: 【Objective】 The objective of this study is to investigate the effect of Kuqin compound on the transcription of Bcl-2/BAX mRNA in porcine kidney cells (PK-15) infected with transmissible gastroenteritis virus (TGEV) . 【Method】 To study the drug (Kuqin compound) test in vitro by TGEV infected PK-15 cell model, the test was divided into Kuqin compound group, blank control group, model group, and astragalus polysaccharide group, respectively. The morphology change was observed in PK-15 cells by the Swiss staining method and the transcription of Bcl-2 and BAX mRNA was tested by RT-PCR method after determination of tissue culture infective dose (TCID50) of TGEV and the maximum of no toxic concentration of Kuqin compound and astragalus polysaccharide on PK-15 cells. 【Result】The following results were obtained from the observation. Compared with blank control group, the PK-15 cells in model group had obvious cytopathic effects (CPE) including elongated cell shape, intercellular drawing into mesh, and lighter staining in nucleus. The nucleus enrichment, pyknosis and fragmentation were also observed. On the other hand, compared with the model group, CPE in Kuqin compound and astragalus polysaccharide group was improved and the Kuqin compound had better effect with most nucleus structure clear, uniform staining in chromatin and fewer nucleuses became larger or fragmentized. The transcription amounts were also considered as an effect of compound Kuqin on infected PK-15 cells. Compared with blank, the transcription amount of Bcl-2 mRNA in model group was less (P＜0.01). However, the transcription amount of Bcl-2 mRNA in compound Kuqin group was increased by 1.403 times compared with the model group (P＜0.01), by 1.078 times with the Astragalus polysaccharide group (P＞0.05). For the BAX mRNA transcription, the model group had an obvious increase (increased by 1.440 times, 1.290 times and 1.490 times compared with the blank group, compound Kuqing group and the astragalus polysaccharide group) while no obvious changes among the other three groups (compound Kuqin was 1.116 larger than blank and astragalus polysaccharide was 0.966 of the blank). Moreover, the transcription ratio of Bcl-2/BAX in model group had a huge decrease compared with the blank group (P＜0.01). This ratio in compound Kuqin and astragalus polysaccharide groups was much larger compared with the model group (P＜0.01) with the number of 1.809 and 1.904 times, respectively; this number was 0.749 (P＜0.01) and 0.933 times (P＜0.05) of blank, respectively.【Conclusion】Kunqin compound could inhibit apoptosis in PK-15 cells infected by TGEV by up-regulating transcription of Bcl-2 mRNA and down-regulating transcription of BAX mRNA.

Key words: Kuqin compound , TGEV , PK-15 , Bcl-2 , BAX , real-time PCR

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