兰州熊蜂气味受体家族鉴定及分析

doi:10.3864/j.issn.0578-1752.2017.10.015

Abstract

Abstract: 【Objective】Bumblebee Bombus lantschouensis is one of the most important pollinators for wild plants and crops in North China. Odorant receptors (Ors) gene family from the genome of B. lantschouensis was identified and characterized. Result of this study will help to explore the function of this gene family in foraging, mating and other social behaviors in bumblebee.【Method】Genomic DNA of thorax of B. lantschouensis was extracted and sequenced by the high-output of next generation sequencing. The original sequence of the sequencing was quality controlled and assembled to the genomic sequence. The contigs and scaffolds were used to build local sequence database. Gene sequences of Bombus terresters and Apis mellifera ligustica were used to query the local database. The characteristics and gene structure of Ors were analyzed by EMBOSS1.5 and GSDS2.0 software, respectively. Conservative motif analysis of amino acid sequences was performed using MEME 4.11.2. The phylogenetic analysis of Ors of B. lantschouensis, B. terresters and A. mellifera was studied by ClustaW 2.1, TrimAl 1.2 and PhyML3.0.【Result】One hundred and sixty-five Ors were identified from the genome of B. lantschouensis, including an Orco, 5 pseudogenes and 159 Ors. Gene structure analysis showed that the number of exons of these Ors are varies from 4 to 9. The least number of exons found in Or 47-57 was 4 and the largest number of exons found in Or 128-161 was 9. According to gene structure, Or was classified into 10 groups. Similarity in exon lengths and numbers was identified in the sequence of each group. The numbers of subgroup members were about 10, except in Or 1-38, Or 69-85 and Or 128-164 (which has 38, 17 and 37 members, respectively). The members of each subgroup are arranged in tandem on chromosomes and Or 1-38 have a longer first exon. Conserved motif analysis revealed that 9 motifs are present in Ors conserved domains (7tm_6 domain) except for motif 5 in all 10 motifs. While Or 1-38 and Or 39-46 have all predicted motifs and motifs 2, 3, 4, and 9 are widely present in sequences, which may be the key functional regions of the family. Phylogenetic analysis showed the 5 subfamilies of Ors family. Whereas the subfamily II contains 2 groups (BlOr 97-100 and BlOr 69-85) and subfamily V contains 4 groups (BlOr 1-46, 47-57, 86-95 and 101-107). BlOr 150-155 and AmOr 122-139 were clustered into two branches, and similar phenomena were also found in BlOr 47-57 and AmOr 63-65. It indicates that Ors has species-specific loss or expansion in evolution of Apis and Bombus. Or 115 is located at the base of the tree, suggesting that the sequence may be closer to the ancestor sequence of the odor receptor family.【Conclusion】Numbers, gene structure and phylogenetic relationships of Ors from the genome of B. lantschouensis were clarified in current study. Conserved motif analysis indicated that motif has been missed during the evolution of Ors family among bees. These results would provide an important information for exploring the gene evolution and function of Ors in future.

Key words: Bombus lantschouensis, odorant receptors, gene structure, conservative motif, phylogenetic analysis

WANG Ye, HAN Lei, DONG Jie, HUANG JiaXing, WU Jie. Identification and Characteristics of Odorant Receptors in Bumblebee, Bombus lantschouensis[J].Scientia Agricultura Sinica, 2017, 50(10): 1904-1913.

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