Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (1): 43-52.doi: 10.3864/j.issn.0578-1752.2014.01.005

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Effect of Potassium Application Time on Starch Accumulation and Related Enzyme Activities of Sweet Potato Variety Jixu 23

 LIU  Hong-Juan-1, YAO  Hai-Lan-1, SHI  Chun-Yu-1, ZHANG  Li-Ming-2   

  1. 1.Agronomy College, Shandong Agricultural University/State Key Laboratory of Crop Biology, Tai’an 271018, Shandong;
    2.Shandong Academy of Agricultural Sciences, Ji’nan 250100
  • Received:2013-04-24 Online:2014-01-01 Published:2013-07-01

RichHTML

13

PDF

706

Cited

7

Abstract: 【Objective】A field experiment was conducted to clarify the regulatory effects of potassium application time on starch accumulation and its physiological mechanism, starch granule distribution of storage roots of sweetpotato variety Jixu 23. 【Method】Jixu 23 (cultivar for starch use) was planted at Tai’an experimental site in Shandong Province, with randomized block design in 2008-2009 growing season. There were four treatments in all, including the control (non-potassium fertilizer, T1), potassium applied as basal fertilizer (T2), as top cover fertilizer (T3) and as top-growth peak fertilizer (T4). Samples were obtained by using periodic sampling during storage roots development. The content of starch, amylose and amylopectin of storage root were determined at different growth stages. Meanwhile the medium diameter of starch grain was determined and the volume fraction of different diameters starch granules (＞19.76 μm, 3.359-19.76 μm and ＜3.359 μm) was calculated. The activities of enzymes (SS, ADPGPPase, UDPGPPase, GBSS and SSS) related to starch synthesis were also studied. The effects of potassium application time on starch and its components, starch granule distribution and activities of enzymes related to starch synthesis in storage roots at different growth stages were analyzed.【Result】Compared with the control (T1), dry matter accumulation and starch yield of storage roots in treatments with potassium applied increased, and the increase of starch yield of T2, T3 and T4 treatments was 24.90%, 16.57%, and 9.24%, respectively. And the increase decreased when the application time delayed. The amylose content of storage root in treatments of topdressing (T3, T4) increased while amylopectin content was decreased significantly. The volume fraction of large starch granules in treatment with potassium applied as basal fertilizer (T2) was larger, and the volume fraction of medium and small starch granules in treatment with potassium applied as top-growth peak fertilizer (T4) was larger than that in the other treatments. Potassium application increased the activities of SS, ADPGPPase and UDPGPPase, and enhanced the supply capability of precursors for the synthesis of starch. And the longest action time of potassium on SS and ADPGPPase activities was 130 days, and potassium applied as basal fertilizer (T2) worked effectively mainly at early and middle stages of storage roots development, while treatments of topdressing (T3, T4) worked effectively mainly at middle and late stages of storage roots development. For UDPGPPase activity, the earlier the potassium applied, the longer action time was got. Potassium applied as topdressing (T3, T4) increased SSS activity but reduced GBSS activity of storage roots during the later expanding period. There was a significant positive correlation between total starch content and SS and ADPGPPase activities, and the significant positive correlation was also found between amylose content and GBSS and SS activities. And amylopectin content had a significant positive correlation with ADPGPPase and SSS activities.【Conclusion】Potassium applied as basal fertilizer (T2) and top cover fertilizer (T3) increased dry matter accumulation and starch yield of storage root significantly. Potassium applied as topdressing increased amylopectin content but decreased amylase content significantly. Treatments with potassium applied as basal fertilizer got a higher volume fraction of large starch granules, while treatments with potassium applied as top-growth peak fertilizer got a higher volume fraction of medium and small starch granules. The significant effects of potassium application time on amylase content, amylopectin content and starch yield were closely related with the changes of SS, ADPGPPase, UDPGPPase, GBSS and SSS activities.

Key words: sweet potato , potassium application time , starch content , starch granule distribution , enzymes related to starch synthesis

[1]史春余, 王振林, 余松烈. 土壤通气性对甘薯产量的影响及其生理机制. 中国农业科学, 2001, 34(2): 173-178.

Shi C Y, Wang Z L, Yu S L. Effects of soil aeration on sweet potato yield and its physiological mechanism. Scientia Agricultura Sinica, 2001, 34(2): 173-178. (in Chinese)

[2]林汝湘, 谢春生, 冯祖虾, 黄宏城. 我国南方甘薯品种资源部分营养成分分析研究. 中国农业科学, 1995, 28(4): 39-45.

Lin R X, Xie C S, Feng Z X, Huang H C. A study on several nutritional compositions of sweet potato germplasm in south China. Scientia Agricultura Sinica, 1995, 28(4): 39-45. (in Chinese)

[3]史春余, 王汝娟, 梁太波, 王振林. 食用型甘薯块根碳水化合物代谢特性及与品质的关系. 中国农业科学, 2008, 41(11): 3878-3885.

Shi C Y, Wang R J, Liang T B, Wang Z L. Characterization of carbohydrate metabolism in relation to quality of storage root in edible sweet potato. Scientia Agricultura Sinica, 2008, 41(11): 3878-3885. (in Chinese)

[4]Cooke D, Gidley M J. Loss of crystalline and molecular order during starch gelatinization origin of the enthalpic transition. Carbohydrate Research, 1992, 227: 103-112.

[5]Ramesh M, Ali S Z, Bhattacharya K R. Structure of rice starch and its relation to cooked-rice texture. Carbohydrate Polymer, 1999, 38: 337-347.

[6]史春余, 王振林, 赵秉强, 郭风法, 余松烈. 钾营养对甘薯块根薄壁细胞微结构、14C同化物分配和产量的影响. 植物营养与肥料学报. 2002, 8(3): 335-339.

Shi C Y, Wang Z L, Zhao B Q, Guo F F, Yu S L. Effects of potassium on the parenchyma cell structure of storage root, the distribution of 14C photosynthates and yield in sweet potato. Plant Nutrition and Fertilizer Science. 2002, 8(3): 335-339. (in Chinese)

[7]吴旭银, 张淑霞, 周印富, 王桂玲, 于玉桥. 甘薯“冀审薯 200001”氮磷钾吸收特性的研究. 河北职业技术师范学院报, 2001, 15(3): 1-4.

Wu X Y, Zhang S X, Zhou Y F, Wang G L, Yu Y Q. Study of assimilation characters of sweetpotato “Ji Shenshu 200001” of nitrogen, phosphorus and potassium. Journal of Hebei Vocation-Teachers College, 2001, 15(3): 1-4. (in Chinese)

[8]Kawagoe Y, Kubo A, Satoh H, Takaiwa F, Nakamura Y. Roles of isoamylase and ADP-glucose pyrophorylase in starch granule synthesis in rice endosperm. The Plant Journal, 2005, 42: 164-174.

[9]闫素辉, 尹燕枰, 李文阳, 梁太波, 李勇, 邬云海, 王平, 耿庆辉, 戴忠民, 王振林. 灌浆期高温对小麦籽粒淀粉的积累、粒度分布及相关酶活性的影响. 作物学报, 2008, 34(6): 1092-1096.

Yan S H, Yin Y P, Li W Y, Liang T B, Li Y, Wu Y H, Wang P, Geng Q H, Dai Z M, Wang Z L. Effect of high temperature during grain filling on starch accumulation, starch granule distribution, and activities of related enzymes in wheat grains. Acta Agronomica Sinica, 2008, 34(6): 1092-1096. (in Chinese)

[10]史春余, 姚海兰, 张立明, 柳洪鹃, 张超, 刘桂玲. 不同类型甘薯品种块根淀粉粒粒度的分布特征. 中国农业科学, 2011, 44(21): 4537-4543.

Shi C Y, Yao H L, Zhang L M, Liu H J, Zhang C, Liu G L. Starch granule size distribution in storage roots of different types of sweet potato cultivars. Scientia Agricultura Sinica, 2011, 44(21): 4537-4543. (in Chinese)

[11]黄华宏, 陆国权, 郑遗凡. 不同生育期甘薯块根淀粉糊化特性的差异. 中国农业科学, 2005, 38(3): 462-467.

Huang H H, Lu G Q, Zheng Y F. Variation in root starch gelatinization characteristics during the growth and development of sweetpotato. Scientia Agricultura Sinica, 2005, 38(3): 462-467. (in Chinese)

[12]尹子娟, 郭华春, 李存芝. 施钾对甘薯生长、产量及品质的影响. 云南农业科技, 2011(4): 21-23.

Yin Z J, Guo H C, Li C Z. Effect of potassium application on growth, yield and quality of sweet potato. Yunnan Agricultural Science and Technology, 2011(4): 21-23. (in Chinese)

[13]郑艳霞. 钾对甘薯同化物积累和分配的影响. 土壤肥料, 2004(4): 14-16.

Zheng Y X. The effect of potassium on accumulation and distribution of materials assimilated in sweet potatoes. Soils and Fertilizers, 2004(4): 14-16. (in Chinese)

[14]柳洪鹃, 史春余, 张立明, 张海峰, 王振振, 柴沙沙. 钾素对食用型甘薯糖代谢相关酶活性的影响. 植物营养与肥料学报, 2012, 18(3): 724-732.

Liu H J, Shi C Y, Zhang L M, Zhang H F, Wang Z Z, Chai S S. Effect of potassium on related enzyme activities in sugar metabolism of edible sweet potato. Plant Nutrition and Fertilizer Science, 2012, 18(3): 724-732. (in Chinese)

[15]陆国权, 唐忠厚, 黄华宏. 不同施钾水平甘薯直链淀粉含量和糊化特性的基因型差异. 浙江农业学报, 2005, 17(5): 280-283.

Lu G Q, Tang Z H, Huang H H. Genotype variation in amylose content and starch pasting properties of sweetpotato storage at two K levels. Acta Agriculturae Zhejiangensis, 2005, 17(5): 280-283. (in Chinese)

[16]陈晓光, 史春余, 李洪民, 张爱君, 史新敏, 唐忠厚, 魏猛. 施钾时期对食用甘薯光合特性和块根淀粉积累的影响. 应用生态学报, 2013, 24(3): 759-763.

Chen X G, Shi C Y, Li H M, Zhang A M, Shi X M, Tang Z H, Wei M. Effects of potassium fertilization period on photosynthetic characteristics and storage root starch accumulation of edible sweet potato. Chinese Journal of Applied Ecology, 2013, 24(3): 759-763. (in Chinese)

[17]何照范. 粮油籽粒品质及其分析技术. 北京: 中国农业出版社, 1985: 290-294.

He Z F. Grain Quality and It’s Analysis Technology. Beijing: China Agricultural Press, 1985: 290-294. (in Chinese)

[18]Peng M, Gao M, Abdel-Aal E S M, Hucl P, Chibbar R N. Separation and characterization of A- and B-type starch granules in wheat endosperm. Cereal Chemistry, 1999, 76: 375-379.

[19]Stoddard F L. Survey of starch particle-size distribution in wheat and related species. Cereal Chemistry, 1999, 76(1): 145-149.

[20]Raeker M Ö, Gaines C S, Finney P L, Donelson T. Granule size distribution and chemical composition of starches from 12 soft wheat cultivars. Cereal Chemistry, 1998, 75(5): 721-728.

[21]Douglas C D, Tsung M K, Frederick C F. Enzymes of sucrose and hexose metabolism in developing kernels of two inbreds of maize. Plant Physiology, 1988, 86: 1013-1019.

[22]程方民, 蒋德安, 吴平, 石春海. 早籼稻籽粒灌浆过程中淀粉合成酶的变化及温度效应特征. 作物学报, 2001, 27(2): 201-206.

Cheng F M, Jiang D A, Wu P, Shi C H. The dynamic change of starch synthesis enzymes during the grain filling stage and effects of temperature upon it. Acta Agronomica Sinica, 2001, 27(2): 201-206. (in Chinese)

[23]Nakamura Y, Yuki K. Changes in enzyme activities as associated with carbohydrate metabolism during the development of rice endosperm. Plant Science, 1992, 82: 15-20.

[24]冷寿慈. 生物统计与田间试验设计. 北京: 中国广播电视出版社, 1992: 115-123, 190-192.

Leng S C. Biostatistics and Design of Field Experiment. Beijing: Chinese Broadcast and Television Press, 1992: 115-123, 190-192. (in Chinese)

[25]杨爱梅, 王自立, 王家才. 甘薯平衡施肥与施用钾肥效果的研究. 河北农业科学, 2009, 13(3): 48-50.

Yang A M, Wang Z L, Wang J C. Study on the Effects of balanced fertilization and application of potassium fertilizer on sweet potato. Journal of Hebei Agricultural Sciences, 2009, 13(3): 48-50. (in Chinese)

[26]赵法茂. 小麦淀粉分支酶同工酶遗传多样性及对酶活性和支链淀粉含量的影响[D]. 泰安: 山东农业大学, 2008: 13.

Zhao F M. Genetic diversity of isozyme of starch branching enzyme and the effects on the activity and amylopectin content in wheat  grain [D]. Tai’an: Shandong Agricultural University, 2008: 13. (in Chinese)

[27]Kim S H, Mizuno K, Fujimura T. Regulated expression of ADP glucose pyrophosphorylase and chalcone synthase during root development in sweet potato. Plant Growth Regulation, 2002, 38: 173-179.

[28]Yatomi M, Kubota F, Saito K, Agata W. Evaluation of root sink ability of sweet potato (Ipomoea batatas Lam) cultivars on the basis of enzymatic activity in the starch synthesis pathway. Journal of Agronomy and Crop Science, 1996, 177(1): 17-23.

[29]闫素辉. 小麦胚乳淀粉合成、粒度分布特征及对花后高温的响应[D]. 泰安: 山东农业大学, 2009: 20.

Yan S H. Starch Synthesis and granuie size distribution of developing wheat endosperm in response to postanthesis high temperature [D]. Tai’an: Shandong Agricultural University, 2009: 20. (in Chinese)

[30]李文阳, 闫素辉, 尹燕枰, 李勇, 梁太波, 耿庆辉, 戴忠民, 王振林. 小麦花后弱光引起籽粒淀粉的粒度分布及组分含量的变化. 生态学报, 2009, 29(1): 298-301. (in Chinese)

Li W Y, Yan S H, Yin Y P, Li Y, Liang T B, Geng Q H, Dai Z M, Wang Z L. Starch granule size distribution and starch component content in wheat grain in relation to shading stress after anthesis. Acta Ecologica Sinica, 2009, 29(1): 298-301. (in Chinese)
[1] ZHAO Shan,ZHONG LingLi,QIN Lin,HUANG ShiQun,LI Xi,ZHENG XingGuo,LEI XinYu,LEI ShaoRong,GUO LingAn,FENG JunYan. Effects of Different Drying Methods on Functional Components and Antioxidant Activity in Sweet Potato Leaves [J]. Scientia Agricultura Sinica, 2021, 54(21): 4650-4663.
[2] ZHAO FuMei,WANG Shuang,TIAN YuTing,QIAO Qi,WANG YongJiang,ZHANG DeSheng,ZHANG ZhenChen. An Investigation into Key Factors Influencing the Occurrence of Virus Disease in Sweet Potato [J]. Scientia Agricultura Sinica, 2021, 54(15): 3232-3240.
[3] WANG JinQiang,LI SiPing,LIU Qing,LI Huan. Mechanism of Spraying Growth Regulators to Alleviate Drought Stress of Sweet Potato [J]. Scientia Agricultura Sinica, 2020, 53(3): 500-512.
[4] QIN YanHong,WANG YongJiang,WANG Shuang,QIAO Qi,TIAN YuTing,ZHANG DeSheng,ZHANG ZhenChen. Complete Nucleotide Sequence Analysis and Genetic Characterization of the Sweet potato feathery mottle virus O and RC Strains Isolated from China [J]. Scientia Agricultura Sinica, 2020, 53(11): 2207-2218.
[5] XU Shu,LI Ling,ZHANG SiMeng,CAO RuXia,CHEN LingLing,CUI Peng,Lü ZunFu,WU LieHong,LU GuoQuan. Evaluation of Genotype Differences of Cold Tolerance of Sweet Potato Seedlings by Subordinate Function Value Analysis [J]. Scientia Agricultura Sinica, 2019, 52(17): 2929-2938.
[6] JIANG ShanShan, FENG Jia, ZHANG Mei, WANG ShengJi, XIN ZhiMei, WU Bin, XIN XiangQi. Development of RT-LAMP Assay for Rapid Detection of Sweet potato feathery mottle virus (SPFMV) [J]. Scientia Agricultura Sinica, 2018, 51(7): 1294-1302.
[7] LI HuaWei, LIU ZhongHua, ZHANG Hong, XU YongQing, LI GuoLiang, LIN ZhaoMiao, QIU YongXiang, LUO WenBin, JI RongChang, TANG Hao, QIU SiXin. Mining and Characterization of MicroRNAs Associated with Pathogenicity by Different Sweet Potato Viruses [J]. Scientia Agricultura Sinica, 2018, 51(11): 2094-2105.
[8] WANG ShunYi, LIU Qing, SHI YanXi, LI Huan. Interactive Effects of Nitrogen and Potassium on Photosynthesis Product Distribution and Accumulation of Sweet Potato [J]. Scientia Agricultura Sinica, 2017, 50(14): 2706-2716.
[9] YUE Rui-xue, NIU Fu-xiang, SUN Jian, XU Fei, WANG Hong-yun, ZHU Hong, ZHANG Yi. Establishment of a Viscosity Reduction Process by Enzymatic Pretreatment and Its Application in Ethanol Fermentation from Fresh Sweet Potato Without Adding Water [J]. Scientia Agricultura Sinica, 2016, 49(9): 1757-1766.
[10] LIU Yu-ting, WU Ming-yang, JIN Yan-ling, SHEN Wei-liang, FANG Yang, ZHAO Hai. Lactic Acid Fermentation by Lactobacillus rhamnosus from Sweet Potato Residue [J]. Scientia Agricultura Sinica, 2016, 49(9): 1767-1777.
[11] CUI Shan-shan, MU Tai-hua, SUN Hong-nan, ZHANG Miao, CHEN Jing-wang. Effects of NaCl Concentration and pH Value on the Emulsifying Properties of Sweet Potato Peptides [J]. Scientia Agricultura Sinica, 2016, 49(9): 1778-1786.
[12] ZHANG Yi, WANG Hong-yun, NIU Fu-xiang, SUN Jian, XU Fei, ZHU Hong, YUE Rui-xue. Identification of Purple Sweet Potato Color of Cultivar Ningzi No. 1 by HPLC-QTOF/MS and Its Effect on Preventing Obesity in High-Fat-Diet-Treated Rats [J]. Scientia Agricultura Sinica, 2016, 49(9): 1787-1802.
[13] TANG Dao-bin, ZHANG Kai, Lü Chang-wen, XIE De-bin, FU Ti-hua, WANG Ji-chun. Genetic Linkage Map Construction Based on EST-SSR and Analysis of QTLs for Starch Content in Sweetpotato (Ipomoea batatas (L.) lam.) [J]. Scientia Agricultura Sinica, 2016, 49(23): 4488-4506.
[14] LU Hui-xiang, Lü Chang-wen, WU Zheng-dan, LUO Kai, YIN Wang, YANG Hang, WANG Ji-chun, ZHANG Kai. Development of Detection Method for Sweet potato feathery mottle virus (SPFMV) and Sweet potato chlorotic stunt virus (SPCSV) Through Fluorescence Quantitative RT-PCR [J]. Scientia Agricultura Sinica, 2016, 49(1): 90-102.
[15] WANG Chen-yang, ZHANG Yan-fei, LU Hong-fang, ZHAO Jun-xia, MA Geng, MA Dong-yun, ZHU Yun-ji, GUO Tian-cai, MA Ying, JIANG Yu-mei. Effects of Post-Anthesis Waterlogging, High Temperature and Their Combination on Starch Compositions and Pasting Properties in Wheat Grains [J]. Scientia Agricultura Sinica, 2015, 48(4): 813-820.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd