‘宁紫薯1号’花青素组分鉴定及其对大鼠高脂诱导肥胖的预防效果

doi:10.3864/j.issn.0578-1752.2016.09.015

Abstract

Abstract: 【Objective】Purple sweet potato are the main source for natural antioxidants. This study aims to identify the components of purple sweet potato color of cultivar Ningzi No. 1, and investigate the effectiveness of PSPC for the prevention of obesity and the regulatory mechanism of PSPC in obesity prevention.【Method】 Purple sweet potato color was analyzed using an Agilent 1290 series high-performance liquid chromatography connected to an Accurate-Mass Quadrupole Time-of-Flight/MS (HPLC-QTOF/MS). A pH-differential method was used to determine the total PSPC content. Eight-week-old male Sprague Dawley strain rats were fed on either a normal diet containing 11.4% fat, or a HFD containing 40% fat and intragastrically administered PSPC once a day. Body weights of the rats were measured every week after 6 h of fasting. Activities of serum glucose, triglyceride, and total cholesterol were determined using commercial kits. Serum leptin levels were measured using enzyme-linked immunosorbent assay kits. Expression of leptin and leptin receptor mRNAs in the hypothalamus were detected using Real-time Quantitative PCR. Expression of leptin and its downstream proteins in the hypothalamus were analyzed using Western blotting.【Result】Eleven constituents were separated. Based on previous reports, ten constituents were identified and Peak 1 was newly reported herein. The cyanidin 3-glucoside content of PSPC was 17.4%. After 6 weeks, we first examined the changes in body weight. Our data showed that the effect of middle-dose PSPC (371.6±16.3) g was better than low-dose PSPC (384.0±7.2) g, and the effect of high-dose PSPC (358.2±20.1) g was best. Obesity was prevented and glucose, triglyceride, and total cholesterol induced by the HFD were restored to near-normal levels. Compared with control group rats (2.29±0.28) ng·mL^-1, the levels of serum leptin in HFD group rats significantly increased by 65.50%. Compared with HFD group rats (3.79±0.24) ng·mL^-1, the levels of serum leptin in low (3.13±0.05) ng·mL^-1, middle (2.84±0.12) ng·mL^-1, and high-dose (2.64±0.06) ng·mL^-1 PSPC group rats were significantly decreased. Both of leptin and leptin receptor mRNAs expression in the hypothalamus was upregulated in the different-dose PSPC group compared with that in the HFD group. High-dose PSPC preserved the leptin signaling capability, leading to a decrease in hypothalamic AMPK α activity.【Conclusion】The anti-obesity effects of high-dose PSPC were mediated via a regulation of leptin/AMPKα signaling in the hypothalamus.

Key words: purple sweet potato, anthocyanidin, HPLC-QTOF/MS, obesity, leptin, AMPK

ZHANG Yi, WANG Hong-yun, NIU Fu-xiang, SUN Jian, XU Fei, ZHU Hong, YUE Rui-xue. Identification of Purple Sweet Potato Color of Cultivar Ningzi No. 1 by HPLC-QTOF/MS and Its Effect on Preventing Obesity in High-Fat-Diet-Treated Rats[J].Scientia Agricultura Sinica, 2016, 49(9): 1787-1802.

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Identification of Purple Sweet Potato Color of Cultivar Ningzi No. 1 by HPLC-QTOF/MS and Its Effect on Preventing Obesity in High-Fat-Diet-Treated Rats

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