Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (9): 1791-1797.doi: 10.3864/j.issn.0578-1752.2012.09.014

• HORTICULTURE • Previous Articles     Next Articles

Effect of the Extracted Solution from the Style of Rosa rugosa on the Growth in Vitro of Pollen Tube of R. rugosa and R. chinensis

 DING  Yi-Ming, ZHAO  Lan-Yong, ZHANG  Ling, SUN  Hua-Cai, WANG  Xin-Gang   

  1. 1.山东农业大学林学院，山东泰安271018
    2.山东农业大学园艺科学与工程学院，山东泰安271018
  • Received:2011-12-30 Online:2012-05-01 Published:2012-03-15

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Abstract: 【Objective】The effect of the extracted solution from the style of Rosa rugosa on the growth in vitro of pollen tube of R. rugosa and R. chinensis was observed to probe the incompatible mechanism of interspecies cross between R. rugosa and R. chinensis.【Method】The pollens of R. rugosa and R. chinensis were cultured by using the extracted solution from the style of R. rugosa, with the apical vesicle of pollen tube marked by FM4-64 and the apical calcium marked by Fluo-3AM, then observed under the laser confocal microscope. The morphological changes of pollen tube were observed by optical microscope.【Result】Treated by the extracting solution from the style of R. rugosa, the pollen tube form of R. rugosa was normal, while that of R. chinensis was distorted and crazed during the pollen tube growth course. The regional distribution of the apical vesicle of pollen tubes was not obvious, calcium concentration declined, calcium gradient disappeared, calcium wave weakened, and some cell walls of the pollen tube tips thickened and resulted in callose.【Conclusion】The extracted solution from the style of R. rugosa affects the apical vesicle secretion and calcium distribution of pollen tube in R. chinensis, so that the growth of pollen tube is prevented and a large amount of callose is produced on the tip of the pollen tube. The result suggests that one reason of the incompatibility of interspecies cross between R. rugosa and R. chinensis may be the effect of R. rugosa style. The style of R. rugosa may affect the apical vesicle secretion and calcium distribution of pollen tube in R. chinensis to prevent its growth, besides, there is a large amount of callose produced on the top of the stagnant pollen tube.

Key words: Rosa, extracted solution from style, pollen tube, interspecies cross

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