Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (22): 4679-4687.doi: 10.3864/j.issn.0578-1752.2013.22.005

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Morphology and Physiological Characteristics of Cultivars with Different Levels of Cold-Resistance in Winter Rapeseed (Brassica campestris L.) During Cold Acclimation

 LIU  Zi-Gang, SUN  Wan-Cang, YANG  Ning-Ning, WANG  Yue, HE  Li, ZHAO  Cai-Xia, SHI  Peng-Fei, YANG  Gang, LI  Xue-Cai, WU  Jun-Yan, FANG  Yan, ZENG  Xiu-Cun   

  1. College of Agronomy, Gansu Agricultural University/Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement/Key Laboratory of Arid Land Crop Science in Gansu Province, Lanzhou 730070
  • Received:2013-02-18 Online:2013-11-15 Published:2013-06-14

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Abstract: 【Objective】The objective of this study was to provide an evaluation method for the development of cold tolerant Brassica campestris germplasm. 【Method】 In this study, the plant morphological characteristics and physiological parameters were measured, and the molecular mechanism of cold-resistance of several cultivars was discussed. 【Result】 Under low temperature, the majority of the stomata in cold sensitive cultivars were closed or semi-closed. But the stomata in strong cold-tolerant cultivars (Longyou 6) maintained opening. The photosynthetic rate and root/shoot ratio were higher, dry matter accumulation in root of Longyou 6 was higher than those of other cultivars. Simple correlation analysis indicated that the soluble protein content was positively correlated with cold-tolerance, but no significant correlation was observed between cold-tolerance and SOD activity, POD activity, CAT activity, free proline content or MDA content. At low temperature, in comparison with cold tolerant variety Tianyou 2, the ice crystals in apoplast proteins extracting solution of ultra strong cold resistant variety Longyou 7 were slight and symmetrical. The shape of ice crystal was pentagon, elliptocytosis or bipyramidal. 【Conclusion】 During the pre-winter cold acclimation stage, the soluble protein content in leaves of strong cold-tolerant winter rapeseeds was high, and the majority of stomata in leaves remained opening, the photosynthetic rate was high, too. Photosynthates might be preferentially transported and stored in the roots. The expression of the antifreeze proteins (AFPs) was up-regulated in roots, which might ease the damage to cells in roots caused by restraining extracellular frozen.

Key words: winter rapeseed(Brassica compestris L.) , cold-resistance , morphological and physiological characters , photosynthetic characteristics , antifreeze protein

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