Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (21): 4478-4485.doi: 10.3864/j.issn.0578-1752.2013.21.010

• SPECIAL FOCUS: STUDIES ON CLIMATE CHANGE IMPACT ON CROP AND ANIMAL PRODUCTION FROM CHINA • Previous Articles     Next Articles

Effect of Heat Stress on Rumen Epithelial Cell Barrier Permeability in Dairy Goat

 MA  Yan-Fen-1, CHEN  Qi-2, DU  Rui-Ping-1, GAO  Min-1   

  1. 1.Animal Nutrition and Feed Research Institute, Inner Mongolia Academy of Agriculture and Animal Husbandry, Hohhot 010031
    2.Research Center of Molecular Biology, Inner Mongolia Medical University, Hohhot 010059
  • Received:2013-05-31 Online:2013-11-01 Published:2013-09-11

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Abstract: 【Objective】Effect of heat stress on rumen epithelial cell barrier permeability in dairy goat were studied in order to provide a rational and experimental basis for animals in hot environments to maintain normal physiological function and to explore methods of resistance to heat stress. 【Method】Effect of heat stress on the concentration of D- lactic acid, DAO, endotoxin and inflammatory cytokines of blood serum, and the expression of tight junction protein occludin were studied with mid- or late-lactation dairy goats by molecular biology method in this project. 【Result】 Heat stress significantly increased the concentration of D- lactic acid, DAO of blood serum. Heat stress significantly increased the concentration of endotoxin and inflammatory cytokines, such as TNF-α, IL-1α, IL-6, IL-1β and interferon-γ. Heat stress damaged the tight junction structure between rumen epithelial, and significantly reduced the proteins expression and mRNA expression of occludin protein in tight junction. 【Conclusion】 The mechanisms of the damage of rumen mucous membrane barrier function, the increase of rumen mucous membrane permeability and the occurrence of bacterial translocation by heat stress may be due to the declined expression of tight junction protein occludin mRNA.

Key words: heat stress , dairy goats , rumen epithelial cell , barrier permeability

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