Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (20): 4263-4271.doi: 10.3864/j.issn.0578-1752.2013.20.009

• PLANT PROTECTION • Previous Articles     Next Articles

Cloning and Localization of an Odorant Receptor Gene OR18 in the Antenna of Spodoptera exigua

 LIU  Cheng-Cheng-12, LIU  Yang-2, ZHANG  Jin-12, WANG  Gui-Rong-2, DONG  Shuang-Lin-1   

  1. 1.Education Ministry Key Laboratory of Integrated Management of Crop Disease and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095
    2.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193
  • Received:2013-03-11 Online:2013-10-15 Published:2013-05-13

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Abstract: 【Objective】 The objective of this study is to clone odorant receptor gene and investigate its expression in different tissues and antennal sensilla of Spodoptera exigua adults, and thus to explore the function of the odorant receptor gene. 【Method】 The full length cDNA sequence encoding SexiOR18 was cloned by homologous-based PCR and RACE techniques. The expression level of this gene in different tissues was investigated by quantitative real-time PCR. The in situ hybridization was used to examine its localization in different antennal sensilla of adult antennae.【Result】 A 740 bp fragment of a OR candidate gene was obtained from the antenna of S. exigua by using homologous-based cloning strategy. The full-length cDNA sequence encoding this gene was further obtained by using RACE technique. The gene was named as SexiOR18 (GenBank accession number: JN873314). The full-length cDNA of SexiOR18 is 1 618 bp with an open reading frame of 1 194 bp, which encodes a polypeptide of 398 amino acids. The multiple alignment and phylogenetic tree analyses showed that SexiOR18 shared high identity with the homologs from other Lepidopteran species, especially noctuid insects. The expression measurements revealed that SexiOR18 expressed in significantly higher level in female antenna than in male antenna, and within the antenna, the expression was mainly restricted under trichoid sensilla and basiconic sensilla, but no expression under coeloconic sensilla and chaetica sensilla.【Conclusion】The SexiOR18 localizes under both trichoid sensilla and basiconic sensilla, suggesting its involvement in detection of both pheromone and general odorants.

Key words: Spodoptera exigua , antenna , sensilla , odorant receptor , quantitative real-time PCR , in situ hybridization

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