Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (19): 4035-4043.doi: 10.3864/j.issn.0578-1752.2013.19.009

• PLANT PROTECTION • Previous Articles     Next Articles

Isolation and Expression Patterns of Rice WRKY80 Transcription Regulatory Protein Gene

 PENG  Xi-Xu, TANG  Xin-Ke, ZHOU  Ping-Lan, HU  Yao-Jun, DENG  Xiao-Bo, WANG  Hai-Hua   

  1. School of Life Sciences, Hunan University of Science and Technology, Xiangtan 411201, Hunan
  • Received:2013-04-10 Online:2013-10-01 Published:2013-05-31

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Abstract: 【Objective】 The objective of this study is to isolate rice WRKY80, to analyze the characteristics of its coding sequence and to investigate its expression patterns in different organs and under pathogen inoculation and hormone application, thus providing a basis for its function identification. 【Method】Specific primer sequences were designed according to the annotated gene Loc_Os03g63810 released in rice genome database. RT-PCR was used to amplify WRKY80 cDNA sequence from RNA pools generated from methyl jasmonate (MeJA)-treated rice leaves. Bioinformatical tools were employed to analyze its deduced protein sequence and cis-elements in its promoter. Northern blot or real-time fluorescence quantification PCR was used to investigate its expression patterns. 【Result】 The obtained cDNA sequence of WRKY80 was 1 392 bp in length, containing an entire open reading frame of 1 164 bp, encoding a polypeptide of 387 amino acid residues consisting of one classic conserved WRKY domain with a zinc finger motif of C2H2, belonging to the WRKY subgroup Ⅱ. WRKY80 possessed an acidic C terminus with consecutive 6 glutamines and 8 threonines, an acidic region possibly responsible for transcription activating activity, and was predicted to be localized in nucleus. WRKY80 shared high identity at the amino acid level with those from monocotyledons Zea mays and Sorghum bicolor. WRKY80 was constitutively expressed in all tested organs. The transcript abundance was relatively higher in leaves, roots and panicles, next was in flowers, and less was in stems and grains. WRKY80 expression was higher in mature leaves and roots than in young leaves and roots, respectively, indicating a developmental stage-related feature. It was rapidly induced by inoculation with Magnaporthe oryzae and Rhizoctonia solani, and also by application of exogenous MeJA and ethephon, whereas salicylic acid exerted no effects on its expression. Consistent with the expression profiles was the prediction result of cis-elements in its promoter. 【Conclusion】WRKY80 possesses structure characteristics as a putative transcription factor. These findings suggest that it may be involved in defense response to the fungal pathogens and also in development regulation by jasmonic acid/ethylene-dependent signaling pathway.

Key words: WRKY transcription factor , fungal pathogen , gene isolation , gene expression pattern , Oryza sativa

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