Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (19): 4027-4034.doi: 10.3864/j.issn.0578-1752.2013.19.008

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Study on Correlation Between Ammonia Volatilization and Nitrogen Metabolism During the Tobacco Leaves Senescence

 WU  Yun-Jie, YANG  Tie-Zhao, ZHANG  Xiao-Quan, LI  Fei, LI  Li-Hua   

  1. College of Tobacco, Henan Agricultural University, Zhengzhou 450002
  • Received:2013-03-06 Online:2013-10-01 Published:2013-07-04

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Abstract: 【Objective】The objective of this experiment is to study the difference of different N efficiency varieties of flue-cured tobacco leaf senescence ammonia volatilization amount in the process, reveal tobacco varieties N transport law, so as to provide a basis for nitrogen resistant variety breeding.【Method】Using ammonia collection device was used to determine the amount of ammonia volatilization of tobacco. At the same time, leaves NH4+ concentration, total N and soluble protein content, N metabolism-related enzyme activity, and apoplast related indicators were measured.【Result】Tobacco amount of ammonia volatilization showed a trend of first increase and then decrease, the maximum at leaf age of 60 d. The amount in varieties Zhongyan90, K326 and NC89, respectively, reached (16.39±1.08) μg•m-2•h-1, (9.92±1.04) μg•m-2•h-1, and (4.56±0.63) μg•m-2•h-1, respectively, 3.87, 2.41 and 2.56 times of the leaf age 40 d ammonia volatilization, and the difference among the varieties was significant. The amount of ammonia volatilization and glutamine synthetase (GS) activity were significantly and negatively correlated, and very significantly and positively correlated with the glutamate dehydrogenase (GDH) activity, apoplastic NH4+ concentration and pH, ammonia compensation point, while very significantly and negatively correlated, with the content of soluble protein and nitrate reductase (NR) activity, They showed a quadratic curve with the leaves NH4+ concentration, and the correlation was extremely significant, and are subject to total N degradation of indirect control. 【Conclusion】 There is a difference among different nitrogen efficient varieties in nitrogen reassimilation and transfer ability，and it is associated with tobacco leaf N metabolism characteristics. During the tobacco senescence, varieties of low N use efficiency, N absorption and assimilation capacity is weak, the transfer capacity, ammonia volatilization amount is big. Through volatilization of ammonia outside the body, increased the transfer of NH4+, reduced the accumulation of ammonia pollution, so it is an important method for plant N metabolic regulation, which can be used as an evaluation of index of a variety nitrogen efficiency.

Key words: flue-tobacco , ammonia volatilization , N metabolism , NH3 compensation point

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