Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (15): 3277-3284.doi: 10.3864/j.issn.0578-1752.2013.15.023

• RESEARCH NOTES • Previous Articles     Next Articles

Molecular Cloning of Sugarcane Cu/Zn Superoxide Dismutase (Cu/Zn-SOD) and Its Expression Analysis

 WANG  Sheng-1, ZHANG  Bao-Qing-1, HUANG  Xing-12, FAN  Yan-Jiao-1, YANG  Li-Tao-1, LI  Yang-Rui-12   

  1. 1.College of Agriculture, Guangxi University/State Key Laboratory for Conservation and Utilization of Subtropical Agro- Bioresources, Guangxi University, Nanning 530004
    2. Sugarcane Research Center, Chinese Academy of Agricultural Sciences/ Sugarcane Research Center, Guangxi Academy of Agricultural Sciences/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture/Guangxi Crop Genetic Improvement and Biotechnology Laboratory/Guangxi Key Laboratory of Sugarcane Genetic Improvement, Nanning 530007
  • Received:2013-01-25 Online:2013-08-01 Published:2013-05-06

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Abstract: 【Objective】The aim of this study was to clone the full-length cDNA of a key enzyme gene Cu/Zn-SOD related to superoxide anion removal in sugarcane (Saccharum spp.), investigate its sequence characteristics and analyze its expression in different organs and under different stresses. 【Method】 The Cu/Zn-SOD gene cDNA sequence was cloned from the leaves of sugarcane variety GT28 using RT-PCR techniques. The bioinformatics methods were used to analyze the putative amino acid sequence, and real-time fluorescence quantitative PCR (qRT-PCR) method was used to study the expression of Cu/Zn-SOD gene in different tissues and under different stresses. 【Result】 The full-length cDNA of Cu/Zn-SOD (GenBank accession number: JQ958328) in sugarcane was cloned. The sequence included an open reading frame of 456 bp, encoding a polypeptide of 151 amino acids, which was clustered in the same phylogenetic branch of the Cu/Zn-SODs of gramineae. Real-time fluorescence quantitative PCR results showed that the Cu/Zn-SOD expressed in root, stem and leaf of sugarcane plant, belonging to constitutive expression, but showing the highest in leaf. The Cu/Zn-SOD expression could be induced under the treatments of polyethylene glycol (PEG), H2O2, low temperature and NaCl stresses, but the expression models were different depending on the regulation mechanisms. 【Conclusion】 The gene Cu/Zn-SOD was cloned, and it mainly expressed in the green tissue in sugarcane plant. Its copper- zinc superoxide dismutase functional areas are highly conservative, and is possibly associated with osmotic stress resistance of sugarcane.

Key words: sugarcane (Saccharum spp.) , Cu/Zn-SOD , adverse stress , expression analysis , real-time fluorescence quantitative PCR (qRT-PCR)

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