Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (14): 3022-3031.doi: 10.3864/j.issn.0578-1752.2013.14.018

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Association of Single Nucleotide Polymorphism of H-FABP and HSL Genes with Growth Traits in Maiwa Yak

 CHAI  Zhi-Xin-1, WANG  Yong-1, LUO  Xiao-Lin-2, WU  Zhi-Juan-1, LIU  Zhong-Na-1, ZHONG  Jin-Cheng-1   

  1. 1.Key Laboratory of Animal Genetics and Breeding, Southwest University for Nationalities, State Ethnic Affairs Commission and Ministry of Education, Chengdu 610041
    2.Sichuan Academy of Grassland Science, Chengdu 610041
  • Received:2013-01-15 Online:2013-07-15 Published:2013-04-07

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Abstract: 【Objective】Correlations between SNP of Maiwa yak heart fatty acid-binding protein (H-FABP), hormone-sensitive lipase (HSL) genes and growth traits were studied in order to provide a theoretical basis for the two candidate genes in expression regulation in the yak. 【Method】Maiwa yak and partial sequences of H-FABP and HSL genes were used as experimental samples and candidate genes. The genetic diversity of Maiwa yak was studied by PCR-SSCP, and the alleles frequency, genotype frequency, equilibrium analysis of Hardy-Weinberg, heterozygosity (H), effective numbers of alleles (Ne) and polymorphism information content (PIC) by means of statistics method. The correlation between the candidate genes in different genotypes and the circumference of growth traits was analyzed.【Result】Polymorphism of H-FABP and HSL genes of some exon portions in Maiwa yak were found. There are three genotypes. The fitness test showed that only HSL gene exon 8 on polymorphic loci in Hardy-Weinberg equilibrium state and the rest of the polymorphic loci were deviation. H-FABP gene exon7 loci existed T→C mutation of 7 339 bp which didn't result in change of amino acid. HSL gene exon7 loci existed in G→C mutation of 6 883 bp, A→G mutation of 6 816bp which led to amino acid from D to G. Analysis indicated that interaction effect of the polymorphism loci genotype and growth traits was significantly (P＜0.01). And A→G mutation of 10 183 bp in exon 8 which led to amino acid from C to G.【Conclusion】H-FABP and HSL genes in Maiwa yak have genetic polymorphism. A6816G genotype can be used as molecular marker.

Key words: Maiwa yak , H-FABP gene , HSL gene , polymorphism

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