Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (13): 2717-2729.doi: 10.3864/j.issn.0578-1752.2013.13.010

• HORTICULTURE • Previous Articles     Next Articles

Evolutionary Studies of S-RNase Genes in Genus Malus

 TANG  Liang, MA  Xiang, LI  Ming-Xia, ZHOU  Zhi-Qin   

  1. 1.Key Laboratory of Horticulture Science for Southern Mountainous Regions，Ministry of Education, Southwest University, Chongqing 400715
    2.College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716
  • Received:2013-01-07 Online:2013-07-01 Published:2013-05-06

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Abstract: 【Objective】By analyzing the sequences of S-RNase gene at the self-incompatibility loci, its evolutionary history, sequence divergence and genetic polymorphism were studied for species belonged to genus Malus. 【Method】All of the Malus S-RNase genes were retrieved from GenBank by NCBI BLAST using the known S-RNase genes of Malus domestica, except four S-RNase genes of M. kansuensis and M. toringoides which were obtained by PCR amplification and clone sequencing. The evolutionary history of the Malus S-RNase genes was studied by phylogenetic analysis, and the characteristics of sequence divergence were analyzed. The genetic polymorphisms and divergence were estimated for different taxa of Malus.【Result】The S-RNase genes of Malus can be divided into 16 clearly delimitated subgroups, the divergence of S-RNase genes within subgroups was low, while it was high among subgroups. There were 50% dN/dS estimations that were greater than 1 among S-RNase gene pairs, and S-RNase genes possessed multiple codons of which dN/dS ratio was greater than 1. There was no difference in the genetic polymorphism between M. domestica and wild Malus species, and the closet taxa with M. domestica was M. sieversii based on divergence of S-RNase gene.【Conclusion】Adaptive amino acid substitutions play an important role in the sequence divergence of S-RNase in Malus. According to the analysis of S-RNase genes belonging to Malus, it was found that the genetic polymorphism of M. domestica has not been affected by cultivation, and M. domestica was derived from M. sieversii through human domestication.

Key words: Malus , S-RNAse gene , evolutionary history , sequence divergence , genetic polymorphism

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