Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (7): 1463-1469.doi: 10.3864/j.issn.0578-1752.2013.07.017

• VETERINARY SCIENCE • Previous Articles     Next Articles

Molecular Characteristics of Extended-Spectrum β-Lactamases in Clinical Isolates of Proteus mirabilis from Poultry

 PAN  Yu-Shan, YUAN  Li, WU  Hua, LIU  Jian-Hua, LIU  Zhen-Zhen, ZHAO  Yi-Shuang, HU  Gong-Zheng   

  1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002
  • Received:2012-11-08 Online:2013-04-01 Published:2013-03-01

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Abstract: 【Objective】This experiment was conducted to explore the genotpye of extended-spectrum β-lactamases (ESBLs) gene and the genetic environment of blaCTX-M carried by Proteus mirabilis. 【Method】The isolates were characterized by isolation and identification, antimicrobial susceptibility testing, confirmation of ESBLs, resistant genes screening, and conjugation experiments. PCR mapping was performed to investigate the genetic environment of blaCTX-M. 【Result】 Among the 21 P. mirabilis clinical isolates from poultry, 10 (47.6%) isolates exhibited extended-spectrum β-lactamase (ESBL) phenotypes. The most common types of ESBL gene screened by PCR and sequencing experiments were blaCTX-M-14 (n=6) , blaOXA-1 (n=6), followed by blaCTX-M-65 (n=4). The blaOXA-10 (n=1) was also detected, while blaSHV was not detected. Nucleotide sequence analysis revealed that insertion sequence ISEcp1B elements present in the upstream region of blaCTX-M genes in all ESBL-producing P. mirabilis, and IS903D was also detected in downstream of CTX-M-9 group genes.【Conclusion】This is the first report the blaCTX-M-65 gene was found in P. mirabilis in China, and the results showed that CTX-M-tpye ESBL in P. mirabilis is no longer an unusual phenomenon in poultry origins.

Key words: Proteus mirabilis , extended-spectrum β-Lactamases , blaCTX-M gene , insertion sequence element , poultry

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