Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (20): 4123-4129.doi: 10.3864/j.issn.0578-1752.2012.20.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Interaction Between AtIPS1 and ATXR5/6 Related to Programmed Cell Death

 MENG  Ping-Hong, ZHAO  Ning, MA  Yu-Hua, CHEN  Zhi-Lin, DENG  Ying, GUO  Jing-Tao, ZHANG  Xing-Guo   

  1. 1.贵州省农业科学院园艺研究所，贵阳 550006
    2.西南大学园艺园林学院/南方山地园艺学教育部重点实验室，重庆 400716
  • Received:2012-04-23 Online:2012-10-15 Published:2012-08-16

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Abstract: 【Objective】The objective of the study was to analyse the localization of AtIPS1 and the interaction between AtIPS1 and ATXR5/6 related to programmed cell death (PCD) in Arabidopsis thaliana. 【Method】The constructs of pAtIPS1::AtIPS1-GFP were introduced in BY-2 cells and plants of Arabidopsis. The fluorescence of GFP was observed under a confocal microscope. The interaction between AtIPS1 and ATXR5/6 was analyzed via Bi-Molecular Fluoresence Complementation (BiFC) and the phenotype of mutant atips1 was observed. 【Result】The results showed that AtIPS1 was accumulated in the cytoplasm and the nucleus. AtIPS1 interacted with ATXR5 and ATXR6. The localisation of AtIPS1 was compatible with an interaction with ATXR5/6. The atips1 mutant displayed the phenotype of the spontaneous lesion formation on leaves. The phenotype of atips1 mutant was complemented and no longer formed lesions by a myo-inositol solution treatment or over-expression the AtIPS1 gene. 【Conclusion】This study indicates that the disruption of AtIPS1 results in cell death on leaves. AtIPS1 accumulated in the cytoplasm and nucleus. AtIPS1 could be involved in the regulation of PCD through the interaction between AtIPS1 and ATXR5/6.

Key words: Arabidopsis thaliana , sub-cellular localization, AtIPS1, protein interaction, programmed cell death (PCD)

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