Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (2): 243-249.doi: 10.3864/j.issn.0578-1752.2013.02.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Effects of 2A Type Protein Phosphatase on the Development of Setosphaeria turcica

 SHEN  Shen, WANG  Jing-Jing, HAO  Zhi-Min, LI  Po, LI  Zhi-Yong, SUN  Zhi-Ying, HAO  Jie, TONG  Ya-Meng, DONG  Jin-Gao   

  1. 1.School of Life Science, Agricultural University of Hebei/Mycotoxin and Plant Molecular Pathology Labortory, Baoding 071001, Hebei Province
    2.Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071001, Hebei
    3.Macun Village Government of Yuanshi County, Shijiazhuang 050035
    4.Institute of Millet Crops, Hebei Academy of       Agricultural and Forestry Sciences, Shijiazhuang 054000
  • Received:2012-11-02 Online:2013-01-15 Published:2013-01-05

PDF

640

Cited

1

Abstract: 【Objective】 The aim of this research is to preliminarily explore the function of type 2A phosphoprotein phosphatase (PP2A) in regulating the development and pathogenicity of S. turcica with the usage of cantharidin, the specific inhibitor of PP2A, and lay a theoretical foundation in researching the new antifungal agents and the prevention of plant diseases. 【Method】 S. turcica was treated with cantharidin at different concentrations to observe and investigate colony growth, conidial yield, conidial germination, appressorium production, melanin biosynthesis and HT-toxin activity of the pathogen. 【Result】 The higher the concentration of cantharidin was, the more strongly the hyphal growth of S. turcica was inhibited. The diameter of 8-day-old colony on the PDA media with cantharidin of 160 μmol•L-1 was 41.7% of that of the mock. Meanwhile, the conidial production in treated groups was higher than that of the CK. When the concentration of cantharidin was up to 160 μmol•L-1, the conidial production was 15.5 times as many as that of the CK. The results of the influences of cantharidin on conidial germination, appressorial formation and invasion showed that the sensitivity of these developmental stages from high to low was appressorial formation > conidial germination > appressorial invasion. Furthermore, the contents of intracellular melanin in treated groups were all higher than that of the CK which was 0.13 g•L-1, and the content of intracellular melanin was positive related with the concentration of cantharidin. 【Conclusion】Cantharidin could significantly inhibit colony growth, conidial germination, appressorium production of S. turcica and promote conidiation and the biosynthesis of intracellular melanin.

Key words: Setosphaeria turcica , PP2A , cantharidin , conidial germination , appressorium , melanin

[1]Schild A, Schmidt K, Lim Y A, Ittner L M, Hemmings B A, Gotz J. Altered levels of PP2A regulatory B/PR55 isoforms indicate role in neuronal differentiation. International Journal of Developmental Neuroscience, 2006, 24: 437-443.

[2]Adams D G, Coffee R L. Positive regulation ofRaf1-MEK1/2-ERK1/2 signaling by protein serine/threonine phosphatase 2A holoenzymes. Journal of Biological Chemistry, 2005, 280: 42644-42654.

[3]Letourneux C, Rocher G, Porteu F. B56-containing PP2A dephosphorylate ERK and their activity is controlled by the early gene IEX-1 and ERK. European Molecular Biology Organization, 2006, 25: 727-738.

[4]Okamoto K, Li H, Jensen M, Zhang T, Taya Y, Thorgeirossore S S, Prives C. Cyclin G recruits PP2A to dephosphorylate Mdm2. Molecular Cell, 2002, 9: 761-771.

[5]Foley T D, Kintner M E. Brain PP2A is modified by thiol-disulfide exchange and intermolecular disulfide formation. Biochemical and Biophysical Research Communications, 2005, 20: 1224-1229.

[6]Mumby M C, Walter G. Protein serine/threonine phosphatases: Structure, regulation and functions in cell growth. Physiological Reviews, 1993, 73: 673-99.

[7]李俊, 苑红燕, 王军, 刘江云, 蔡培烈, 杨世林. 斑蝥中斑蝥素提取工艺研究. 苏州大学学报, 2011, 27: 86-89.

Li J, Yuan H Y, Wang J, Liu J Y, Cai P L, Yang S L. The extraction process of cantharidin in Mylabris cichorii. Journal of Soochow University, 2011, 27: 86-89. (in Chinese)

[8]周玥, 张学景, 蔡于琛, 冼励坚, 邹永. 蛋白磷酸酶1和2A抑制剂的研究进展. 中国药学杂志, 2007, 42: 324-328.

Zhou Y, Zhang X J, Cai Y C, Xian L J, Zou Y. Recent advances in protein phosphatases 1 and 2A inhibitors. Chinese Pharmaceutical Journal, 2007, 42: 324-328. (in Chinese)

[9]曹微丹, 张志勇, 杨宝东, 张民照, 孙淑玲. 斑蝥素及去甲斑蝥素对七种植物病原真菌的抑制作用. 植物保护学报, 2008, 35: 63-68.

Cao W D, Zhang Z Y, Yang B D, Zhang M Z, Sun S L. Inhibition of cantharidin and demethyl cantharidin to seven phytopathogenic fungi. Acta Phytophylacica Sinica, 2008, 35: 63-68. (in Chinese)

[10]Wang H, Jiang Y. The Tap42-protein phosphatase type 2A catalytic subunit complex is required for cell cycle-dependent distribution of actin in yeast. Moleclecular and Cellular Biology, 2003, 23: 3116-3125.

[11]Giesbert S, Schumacher J, Kupas V, Espino J, Segmüller N, Haeuser-Hahn I, Schreier P H, Tudzynski P. Identification of pathogenesis associated genes by T-DNA-mediated insertional mutagenesis in Botrytis cinerea: a type 2A phosphoprotein phosphatase and a SPT3 transcription factor have significant impact on virulence. Molecular Plant-Microbe Interactions, 2012, 25(4): 481-495.

[12]Bashyal B M, Chand R, Kushwaha C, Sen D, Prasad L C, Joshi A K. Association of melanin content with conidiogenesis in Bipolaris sorokiniana of barley (Hordeum vulgare L.). World Journal of Microbiology and Biotechnology, 2010, 26(2): 309-316.

 [13]张利辉, 刘云惠, 董金皋, 李正平. 玉米大斑病菌特异性毒素组分的分离与纯化. 植物病理学报, 2003, 33(1): 67-71.

Zhang L H, Liu Y H, Dong J G, Li Z P. Isolation and purification of specific toxin fractions produced by Exserohilum turcicum. Acta Phytophylacica Sinica, 2003, 33(1): 67-71. (in Chinese)

[14]Lechward K, Awotunde O S, Swiatekw, Muszyńska G. Protein phosphatase 2A: Variety of forms and diversity of functions. Acta Biochimica Polonica, 2001, 48: 921-933.

[15]Yatzkan E, Yarden O. Inactivation of a single-2A phosphoprotein phosphatase is lethal in Neurospora crassa. Current Opinion in Genetics, 1995, 28: 458-466.

[16]Yatzkan E, Yarden O. The B regulatory subunit of protein phosphatase 2A is involved in different developmental stages in Neurospora crassa. Molecular Microbiology, 1999, 31: 197-210.

[17]Wang H, Wang X, Jiang Y. Interaction with Tap42 is required for the essential function of Sit4 and type 2 phosphatases. Molecular Biology of the Cell, 2003, 14: 4342-4351.

[18]Wang Y, Ng T Y. Phosphatase 2A negatively regulates mitotic exit in Saccharomyces cerevisiae. Molecular Biology of the Cell, 2006, 17: 80-89.

[19]Longin S, Zwaenepoel K, Louis J V, Dilworth S, Goris J, Janssens V. Selection of protein phosphatase 2A regulatory subunit is mediated by the C-terminus of the catalytic subunit. Journal of Biological Chemistry, 2007, 282: 26971-26980.

[20]Joshua D N, Arturo C. Impact of melanin on microbial virulence and clinical resistance to antimicrobial compounds. Antimicrobial Agents and Chemotherapy, 2006, 50(11): 3519-3528.

[21]曹志艳, 杨胜勇, 董金皋. 植物病原真菌黑色素与致病性关系的研究进展. 微生物学通报, 2006, 33(1): 154-158.

Cao Z Y, Yang S Y, Dong J G. A review on relations between pathogenicity and melanin of plant fungi. Microbiology China, 2006, 33(1): 154-158. (in Chinese)

[22]曹志艳, 董金皋, 杨胜勇, 姚晓霞. 玉米大斑病菌黑色素的一些理化性质和光谱吸收特征. 植物病理学报, 2007, 37(4): 410-417.

Cao Z Y, Dong J G, Yang S Y, Yao X X. Characteritics and spectral an alysis of me lanin in Setosphaeria turcica. Acta Phytophylacica Sinica, 2007, 37(4): 410-417. (in Chinese)
[1] HAO YuBin,LI HaiXiao,ZHANG Sai,LIU Ning,LIU YingZi,CAO ZhiYan,DONG JinGao. Identification and Functional Analysis of StSCD Family in Setosphaeria turcica [J]. Scientia Agricultura Sinica, 2022, 55(16): 3134-3143.
[2] LI TianCong,ZHU Hang,WEI Ning,LONG Feng,WU JianYing,ZHANG Yan,DONG JinGao,SHEN Shen,HAO ZhiMin. The Expression Pattern and Interaction Analysis of the Homologues of Splicing Factor SC35 in Setosphaeria turcica [J]. Scientia Agricultura Sinica, 2021, 54(4): 733-743.
[3] LONG Feng,WANG Qing,ZHU Hang,WANG JianXia,SHEN Shen,LIU Ning,HAO ZhiMin,DONG JinGao. Identification and Expression Pattern Analysis of Septin Gene Family of Setosphaeria turcica [J]. Scientia Agricultura Sinica, 2020, 53(24): 5017-5026.
[4] LI Jie,JIA XuChao,ZHANG RuiFen,LIU Lei,CHI JianWei,HUANG Fei,DONG LiHong,ZHANG MingWei. Isolation, Structural Characterization and Antioxidant Activity of Black Sesame Melanin [J]. Scientia Agricultura Sinica, 2020, 53(12): 2477-2492.
[5] FENG ShengZe, LIU XingChen, WANG HaiXiang, ZHAO Jie, ZHAO LiQing, ZHENG YaNan, GONG XiaoDong, HAN JianMin, GU ShouQin, DONG JinGao. Influencing Factors of Conidiospore and Expression Analysis of GATA Transcription Factor Gene Family in Setosphaeria turcica [J]. Scientia Agricultura Sinica, 2017, 50(7): 1234-1241.
[6] ZHAO BingLing, LI YaNan, CHEN TianZhi, LIU Ying, CHANG LuCheng, FAN RuiWen, XUE LinLi, WANG HaiDong, DONG ChangSheng. GPNMB Affects Melanin Synthesis in the Melanocytes via MITF to Regulate the Downstream Pigmental Genes [J]. Scientia Agricultura Sinica, 2017, 50(7): 1334-1342.
[7] ZHAO Jie, ZHAO LiQing, GONG XiaoDong, FENG ShengZe, LIU XingChen, ZHENG YaNan, LI ZhiYong, SUN HaiYue, WANG DongXue, HAN JianMin, GU ShouQin, DONG JinGao. Identification of Homeobox Transcription Factor Family in Genome-Wide and Expression Pattern Analysis of the Members in Setosphaeria turcica [J]. Scientia Agricultura Sinica, 2017, 50(4): 669-678.
[8] SHEN Shen, LI ZhenYang, ZHAO YuLan, LI Pan, HAN JianMin, HAO ZhiMin, DONG JinGao. Cloning and Expression Pattern Analysis of cAMP Phosphodiesterase Coding Genes in Setosphaeria turcica [J]. Scientia Agricultura Sinica, 2017, 50(16): 3135-3144.
[9] ZHANG YunFeng, ZHANG ShuHong, WU QiuYing, FAN YongShan. Effects of STK1 on Glycogen and Lipid Accumulation During the Appressorium Development of Setosphaeria turcica [J]. Scientia Agricultura Sinica, 2017, 50(15): 2928-2935.
[10] GONG XiaoDong, LIU XingChen, ZHAO LiQing, ZHENG YaNan, FAN YongShan, HAN JianMin, GU ShouQin, DONG JinGao. Effect of Hyperosmotic Stress on Growth and Development of Setosphaeria turcica and Determination of Osmolytes in the Mycelium Cells of the Pathogen [J]. Scientia Agricultura Sinica, 2017, 50(10): 1922-1929.
[11] MA Shuang-xin, LIU Ning, JIA Hui, DAI Dong-qing, XU Miao-miao, CAO Zhi-yan, DONG Jin-gao. Analysis and Expression of Laccase Gene Stlac2 in Setosphaeria turcica [J]. Scientia Agricultura Sinica, 2016, 49(21): 4130-4139.
[12] YUE Xiao-feng, QUE Ya-wei, WANG Zheng-yi. Analysis of RNA-Seq-Based Expression Profiles of Δznf1 Mutants in Magnaporthe oryzae [J]. Scientia Agricultura Sinica, 2016, 49(17): 3347-3358.
[13] NIE Rui-qiang, YANG Yu-jing, XIE Jian-shan, FAN Rui-wen, XU Dong-mei, YU Xiu-ju, DUAN Zhi-cheng, DONG Chang-sheng. Influences of Pax6 PAI Subdomain on MITF, TYR, TYRP1 and TYRP2 in Melanocytes [J]. Scientia Agricultura Sinica, 2016, 49(17): 3433-3442.
[14] NIE Rui-qiang, YANG Yu-jing, XIE Jian-shan, FAN Rui-wen, GAO Wen-jun, DONG Chang-sheng. The Influences of Over-Expressing Pax610Neu on MITF and TYR in Melanocytes [J]. Scientia Agricultura Sinica, 2016, 49(11): 2214-2221.
[15] ZHENG Nen-zhu, XIN Qing-wu, ZHU Zhi-ming, MIAO Zhong-wei, Li Li, Liu Feng-hui, HUANG Qin-lou. cDNA Cloning and Expression of MITF Gene and Its Effect on Melanin Deposition in Silky Fowl [J]. Scientia Agricultura Sinica, 2015, 48(18): 3711-3718.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd