Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (22): 4728-4737.doi: 10.3864/j.issn.0578-1752.2012.22.020

• RESEARCH NOTES • Previous Articles     Next Articles

Ananlysis of Differential Stigma Proteins Between a Pollination- Deficiency Mutant in Brassica napus L.and Its Wild Type

 LI  Chun-Hong, FU  San-Xiong, QI  Cun-Kou   

  1. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Sub-center of National Rapeseed Development Center, Nanjing 210014
  • Received:2012-07-13 Online:2012-11-15 Published:2012-10-09

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Abstract: 【Objective】The difference in protein expression was identified between a pollination-deficiency mutant FS-M1 in Brassica napus L. and its wild type at proteomics level. 【Method】 Two-dimensional electrophoresis (2-DE) was used to study the variation expression of stigma proteins between FS-M1 and its wild-type (NY10). The characteristics and functions of proteins expressed up-regulated in the B. napus L. stigma were analyzed with MALDI-TOF-TOF/MS and Protein Data Bank. 【Result】 A total of 33 up-regulated proteins expressed in the wild-type stigma were identified, which were involved in 7 functional categories, including stress response and defense, regulation of redox homeostasis, carbohydrate metabolism, proteolysis proteins, protein folding, amino acid and nitrogen metabolism, nucleotid metabolism, and unclassified. 【Conclusion】 The functions of protein up-regulated in the wild-type stigma were extensive，which could be related to pollination regulation of B. napus L..

Key words: Brassica napus L. , stigma , 2-DE , MALDI-TOF-MS

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