油菜苗期抗旱性评价及抗旱相关指标变化分析

doi:10.3864/j.issn.0578-1752.2013.03.004

Abstract

Abstract: 【Objective】An experiment was conducted to investigate the ability of drought resistance at seedling stage of rapeseed (Brassica napus L.), to explore the parameters and methods for screening of drought-resistant germplasm, and to provide the materials and a theoretical basis for drought-resistant breeding and cultivation.【Method】 Leaf relative water content, malondialdehyde (MDA) and protective enzyme activity in rapeseed were measured under drought stress on 0, 5, 10, 15, 20 and 25 d; Comprehensive drought-resistance coefficient, drought-resistance index, fuzzy clustering and grey correlation analysis were used to evaluate the drought tolerance; And the variation trend of relevant drought-resistance index was analyzed for ten rapeseed varieties. 【Result】 Different examined indexes showed different responses to drought stress, in which, the content of proline and POD activity were retarded, but the relative water content of leaves was sensitive in response to drought stress. According to the clustering result of drought-resistance measuration value (D value), the 10 tested varieties were divided into 3 groups including high, moderate and low drought resistance, respectively. The variation trend of relevant drought-resistance index showed that the decrease rate of leaf relative water content and leaf area became larger with the increase of degree and time of drought stress, but the contents of MDA, proline, soluble sugar and soluble protein and the relative activities of SOD and POD were overall ascendant. The relative value of MDA content was negatively correlated with drought resistance, while the increase rate of relative content of proline, soluble sugar, soluble protein and the activity of POD were positively correlated with that of rapeseed varieties. 【Conclusion】 It is an effective way to evaluate the drought tolerance of rapeseed seedlings by using the combination method of comprehensive drought-resistance coefficient, drought-resistance index, fuzzy clustering and grey correlation analysis, which could reflect the relationship between different parameter traits and drought tolerance in rapeseed. Among the 10 tested varieties, the drought tolerance of 94005, Zhongshuang11 and Zhongshuang9 were better and more stable. The change of drought-resistance related parameters of rapeseed under continuous drought stress presented a certain regularity, which indicated the responses of rapeseed to drought stress and could lay a basis for the screening of rapeseed germplasm.

Key words: rapeseed (Brassica napus L.) , drought-resistance parameters , subordinate function coefficients , fuzzy clustering , grey correlative analysis

XIE Xiao-Yu, ZHANG Xia, ZHANG Bing. Evaluation of Drought Resistance and Analysis of Variation of Relevant Parameters at Seedling Stage of Rapeseed (Brassica napus L.)[J].Scientia Agricultura Sinica, 2013, 46(3): 476-485.

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Evaluation of Drought Resistance and Analysis of Variation of Relevant Parameters at Seedling Stage of Rapeseed (Brassica napus L.)

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