Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (9): 1833-1839.doi: 10.3864/j.issn.0578-1752.2012.09.019

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Proteomic Comparison on Malpighian Tubules Between Larvae Stage and Pupal Stage of Silkworm (Bombyx mori)

 ZOU  Yong, ZHONG  Xiao-Wu, ZHANG  Li-Ping, ZHAO  Ping, XIA  Qing-You   

  1. 1.西南大学蚕学与系统生物学研究所/家蚕基因组学国家重点实验室，重庆 400716
  • Received:2011-07-28 Online:2012-05-01 Published:2011-11-04

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Abstract: 【Objective】 The objective of this study is to get more information of malpighian tubules on the proteome changes between larvae stage and pupal stage, and to provide new evidences on the protein level at different developmental stages of malpighian tubules of B. mori. 【Method】 The matrix-assisted laser desorption ionization time of flight mass spectrometry were applied for identifying the different spots which had more expression of the 2D map, and all the protein sequences from P50/Dazao on NCBI combined silkworm proteins database were used to build local-database by the software GPMAW 8.00. GPMAW was also used to analyze peptide fingerprint masses.【Result】 About 360-430 spots were obtained by silver staining from the malpighian tubules after 2D-PAGE. Most of them were distributed in the area from 15 to 80 kD with pI 4.0-9.5. Seventeen markedly different proteins between larvae stage and pupal stage were successfully identified, including not only the proteins in association with energy metabolism, but also heat shock proteins, vacuolar-type H+-transporting ATPase, 30K lipoprotein, and alanine-glyoxylate aminotransferase 2,3-hydroxyisobutyrate dehydrogenase which have important functions in mammal kidney. 【Conclusion】 The discovery and identification of these proteins can offer a valuable insights into functional differences of larvae stage and pupal stage of holometabolic insects.

Key words: Bombyx mori, malpighian tubule, 2D-PAGE, MALDI-TOF-MS

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