Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (20): 4326-4334.doi: 10.3864/j.issn.0578-1752.2012.20.022

• RESEARCH NOTES • Previous Articles    

Inheritance and Gene Cloning of an ALS Inhabiting Herbicide- Resistant Mutant Line M9 in Brassica napus

 HU  Mao-Long, PU  Hui-Ming, GAO  Jian-Qin, LONG  Wei-Hua, QI  Cun-Kou, ZHANG  Jie-Fu, CHEN  Song   

  1. 1.江苏省农业科学院经济作物研究所/国家油料作物改良中心南京分中心/农业部长江下游棉花与油菜重点实验室,南京 210014
  • Received:2012-06-14 Online:2012-10-15 Published:2012-08-29

Abstract: 【Objective】The objective of this study is to identify and characterize the resistant gene of a mutant line M9,which was previously found in the rapeseed (brassica napus L.) by spontaneous mutation, conferring resistance to acetolactate synthase or acetohydroxyacid synthase (ALS or AHAS) inhibiting herbicides, and understand the molecular basis for resistance to ALS-inhibiting herbicides.【Method】Reciprocal crosses were made in order to study the inheritance of resistance. Three genes BnALS1-BnALS3 encoding ALS were isolated from the mutant and wild type by using the homology-based candidate gene method. The resistant gene of M9 was transformed into Mutsu-Isuzu cytoplasmic male sterile (MICMS) restorer lines by hybridization, microspore culture, and PCR analysis. 【Result】The resistance of M9 was inherited as a single, dominant nuclear gene. Three genes BnALS1-BnALS3 encoding ALS were isolated. Molecular analysis identified a single-point mutation leading to an amino acid substitution from serine 638 (AGT) to asparagine (AAT) at the herbicide-binding site of the rapeseed BnALS1 gene. All the resistant restoring lines had the specific band of BnALS1R by sequencing. 【Conclusion】The resistance trait of M9 is controlled by a single dominant nuclear gene. The molecular basis for the resistance to ALS-inhibiting herbicides results from the point mutation (Ser638Asn).

Key words: herbicide, rapeseed (Brassica napus L.), acetolactate synthase, single-point mutation, BnALS1R

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