Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (22): 4720-4727.doi: 10.3864/j.issn.0578-1752.2012.22.019

• RESEARCH NOTES • Previous Articles     Next Articles

Detection of the Relative Content of Ustiloxin A in Rice False Smut Balls and Correlation Analysis Between Pathogenicity and Ustiloxin A Production of Ustilaginoidea virens

 YIN  Xiao-Le, CHEN  Zhi-Yi, LIU  Yong-Feng, YU  Jun-Jie, LI  Yan, YU  Mi-Na   

  1. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014
  • Received:2012-03-13 Online:2012-11-15 Published:2012-07-11

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Abstract: 【Objective】The objective of this study is to define the active components of crude toxin produced by Ustilaginoidea virens, to analyze the ustiloxin A production of false smut balls in different areas, and to discover the correlation between pathogenicity and ustiloxin A production of the fungus.【Method】The crude toxin of U. virens was extracted from false smut balls by acetone, methanol and water, respectively, and the toxic effect was assayed and indicated by inhibition to wheat seed germination. The active components of the crude toxins were studied by liquid chromatography mass-spectrometry (LC-MS) and UV absorption spectra. Ustiloxin A production of 224 false smut balls collected from different areas in China was analyzed by high performance liquid chromatography (HPLC). The pathogenicity of 52 isolates of U. virens was examined through inoculating the susceptible rice cultivars, Liangyoupeijiu. The correlation between pathogenicity and ustiloxin A production of the fungus was analyzed by using SPSS software.【Result】The crude toxin extracted with water and methanol had higher inhibition to wheat radicle growth with the inhibition rate of 59.6% and 52.9%, respectively, while the crude toxin extracted with acetone had lower inhibition. The molecular weight of the active ingredient Ⅱwas 673.9 by determination with LC-MS. Combined with the UV spectra assay, ingredient Ⅱcould be proved to be ustiloxin A. It was found that there was a significant difference in the ustiloxin A production among false smut balls collected from different areas. However, correlation analysis indicated that no significant correlation between pathogenicity of the fungus and its ustiloxin A production（R=0.222, P=0.114）.【Conclusion】Water extract of false smut balls caused by U. virens contained ustiloxin A. And a significant difference in ustiloxin A production was found in false smut balls collected from different areas. There may be no association between pathogenicity and ustiloxin A production of the fungus.

Key words: Ustilaginoidea virens , crude toxin , ustiloxin A , pathogenicity

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