Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (17): 3453-3462.doi: 10.3864/j.issn.0578-1752.2012.17.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Detection of QTL for Kernel Weight, Grain Size, and Grain Hardness in Wheat Using DH and Immortalized F2 Population

 LI  Wen-Fu, LIU  , BIN  , PENG  , TAO  , YUAN  Qian-Qian, HAN  Shu-Xiao, TIAN  Ji-Chun   

  1. 1.山东农业大学农学院/作物生物学国家重点实验室/山东省作物生物学重点实验室，山东泰安 271018
    2.山东省农业科学研究院，济南 250000
    3.济源市农业科学研究所，河南济源 454652
  • Received:2012-03-30 Online:2012-09-01 Published:2012-06-04

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Abstract: 【Objective】 Grain weight (GW), grain size (GS), and grain hardness (HD) are important complex traits in wheat, which are determined by quantitative trait loci (QTLs). In this study, to attain more genetic information of GW, GS, and HD in wheat, QTLs with additive effects and dominant effects for these traits were detected. 【Method】 Based on a genetic linkage map contains 368 sites, QTLs for GW, GS, and GS were evaluated in five different environments by using a doubled haploid (DH) population lines derived from a cross between two elite Chinese wheat cultivars Huapei 3/Yumai 57 (Triticum aestivum L.) and in two different environments by using an immortalized F2 population generated by randomly permutated intermating of these DHs. 【Result】 A total of 32 additive QTLs and 18 pairs of epistatic QTLs were detected, including 8 additive QTLs and 5 pairs of epistatic QTLs for 1000-kernel weight, 10 additive QTLs and 6 pairs of epistatic QTLs for grain length , 10 additive QTLs and 6 pairs of epistatic QTLs for grain diameter, and 7 additive QTLs and 1 pair of epistatic QTLs for grain hardness. The QTL Qtkw6A for GW was persistently expressed in both of DH and IF2 population, and the contributions of additive effect in the two population were 9.39% and 11.75%, respectively, and 1.37% for that of dominance effect. The QTL Qgd6A for grain diameter was persistently expressed in both of DH and IF2 population, and the contributions of additive effect in DH and IF2 population were, respectively, 15.02% and 15.03%, It was the same as the QTL Qgl6A, the contributions of additive effect in DH and IF2 population were, respectively, 14.96% and 15.10%. 【Conclusion】The results showed that both additive effects and epistatic effects were important genetic bases of GW and GS. Grain hardness is mainly controlled by a major gene located on the short arm of chromosome 5D, and is also influenced by other minor genes and epistatic effects. The QTL detected in this study could be used in MAS. The QTL with dominance effect and those with interactions between dominance and additive, additive and dominance, dominance and dominance detected by IF2 population would provide assistance for the study on heterosis.

Key words:

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