Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (16): 3289-3296.doi: 10.3864/j.issn.0578-1752.2011.16.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Molecular Identification of Hardness-related Genes of Bread Wheat New Lines in Huanghuai Wheat Region and Their Influence on Yield Characters

ZHANG  Fu-Yan, CHEN  Feng, DONG  Zhong-Dong, SHANG  Xiao-Li, CUI  Dang-Qun, YIN  Gui-Hong   

  1. 1.河南农业大学农学院/河南省粮食作物生理生态与遗传改良国家重点实验室培育基地
    2.周口市农业科学院
  • Received:2010-12-13 Revised:2011-01-24 Online:2011-08-15 Published:2011-03-08

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Abstract: 【Objective】 Identification of hardness-related genes in wheat new lines or cultivars developed very recently in Huang-Huai Wheat Region of China could illustrate the distribution of hardness-related genes and provide important gene germplasm for improvement of wheat quality. 【Method】 A total of 109 wheat cultivars or advanced lines widely grown in Huang-huai wheat region were used to identify the phenotype of grain texture and genotype of puroindoline and puroindoline b-2 genes by the technologies of Single Kernel Characterization System (SKCS), PCR amplification with specific primers, restriction enzyme and DNA sequencing. 【Result】 The results showed that hard wheat with percentage of 61.5% was predominant while percentages of the mixed and soft wheats were only 15.6% and 22.9%, respectively, in wheat new lines surveyed. Based on SKCS results, grain hardness index of wheat surveyed ranged widely from 3.2 to 82.6. Three puroindoline alleles of Pinb-D1b, Pinb-D1p and Pina-D1b were detected in hard wheat surveyed. Of them, Pinb-D1b is the most prevalent with percentage of 86.5 %, while Pinb-D1p and Pina-D1b were only 7.5% and 6.0%, respectively. Furthermore, based on identification of puroindoline b-2 gene with gene-specific primers, all surveyed bread wheat genomes D and A contained Pinb-D2v1 and Pinb-A2v4 variants, respectively. However, Pinb-B2v3 variant was only identified in the group B of 86 wheat cultivars or lines surveyed, and Pinb-B2v2 variant was found in the group B of remaining 23 cultivars or lines. The results of analysis of yield characters between Pinb-B2v2 and Pinb-B2v3 variants indicated that the averages of grain number, single grain weight, flag leaf length and flag leaf area of genotypes with Pinb-B2v3 variant were significantly higher than those of genotypes with Pinb-B2v2 variant. 【Conclusion】 Hard wheat was predominant in the wheat germplasms surveyed and Pinb-D1b was the most popular in hard wheat lines. In addition, the wheat with Pinb-B2v3 possessed relatively superior yield character to wheat with Pinb-B2v2.

Key words: Bread wheat, Grain hardness, Puroindoline, Puroindoline b-2, Yield-related traits

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