Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (15): 3078-3085.doi: 10.3864/j.issn.0578-1752.2011.15.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Dynamic QTL Mapping of Wheat Protein Content in Developing Grains

ZHU  Zhan-Ling, LIU  Bin, TIAN  Bin, XIE  Quan-Gang, LI  Wen-Fu, TIAN  Ji-Chun   

  1. 1.山东农业大学农学院/作物生物学国家重点实验室小麦品质育种研究室
  • Received:2010-12-07 Revised:2011-01-06 Online:2011-08-01 Published:2011-01-30

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Abstract: 【Objective】Grain protein content (GPC) is an important quality factor in bread wheat, which is determined by quantitative trait loci (QTLs). In this study, to attain more genetic information of GPC accumulation，the number and genetic effects of QTLs/genes related to GPC were detected at different seed filling stages. 【Method】 QTLs/genes related to GPC were studied at five seed filling stages in six different environments, using a doubled haploid (DH) population derived from a cross between two elite Chinese wheat cultivars Huapei 3×Yumai 57. QTL mapping for developmental behavior of GPC was studied by unconditional and conditional quantitative trait locus (QTL) analyses in a mixed linear model.【Result】A total of nine unconditional QTLs and ten conditional QTLs were detected at five seed filling periods. The unconditional QTL named QGpc3A persistently expressed at all seed filling stages may play a most important role for the accumulation of GPC, the rest unconditional QTLs and conditional QTLs were only detected at a few or a special periods. At the period of 12 days after anthesis the gene expression was active, and the QTLs detected could account for 42.62% of phenotypic variation totally. However, the QTLs detected could totally account for only 17.43% of phenotypic variation at 22 d after anthesis with lowest phenotypic value. 【Conclusion】The dynamic change of GPC show a high - low - high trend and QTLs controlling GPC were developmental stage speci?c.

Key words: common wheat, DH population, grain protein content (GPC), unconditional QTL, conditional QTL

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