Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (15): 3020-3028.doi: 10.3864/j.issn.0578-1752.2012.15.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Embyo-Specific Expression of Tomato SlABI3 Results in Inhibition of Preharvest Sprouting in G46B of Rice

 XIAO  Ying-Yong, GAO  Yong-Feng, TANG  Wei, YU  Jin-De, LIU  Liang-Liang, ZHANG  Xia, MENG  Meng, LIU  Yong-Sheng   

  1. 四川大学生命科学学院/水力学与山区河流开发保护国家重点实验室/生物资源与生态环境教育部重点实验室，成都 610064
  • Received:2012-03-09 Online:2012-08-01 Published:2012-05-10

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Abstract: 【Objective】In order to increase resistance to preharvest sprouting in maintain line G46B of rice, the promoter of rice OsVP1 was used to drive tomato SlABI3 (homologous to OsVP1) expression in rice embryos. This will probably provide a theoretical basis of molecular design for improving preharvest sprouting resistance in hybrid rice.【Method】The expression vector containing the full-length coding region of SlABI3 whose expression is driven by the OsVP1 promoter was constructed and introduced into rice G46B by Agrobacterium-mediated genetic transformation. To further elucidate the physiological function of SlABI3 in inhibiting the pre-harvest sprouting, the phenotypic differences in germination between transgenic and wild-type seeds were observed. 【Result】A number of transgenic plants containing pHB-OsVP1P::SlABI3 were obtained. Germination experiments showed that the percentage of geminated seeds from transgenic plants was lower than that of wild-type G46B plants, and that the shoot and root length of transgenic seedling was significantly shorter than that of wild-type seedlings 8 days after germination. In addition, in the experiment of using different concentrations of ABA treatments, although increasing ABA application resulted in decrease of germination rate in both the transgenic and wild-type seeds, germination velocity of the transgenic seeds was slower than that wild-type seeds. 【Conclusion】 Embryo-specific expression SlABI3 in rice can reduce germination velocity of transgenic seeds and also inhibit the preharvest sprouting in hybrid rice lines.

Key words: Oryza sativa L., SlABI3, OsVP1, ABA, endosperm specific expression, preharvest sprouting

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