Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (13): 2728-2736.doi: 10.3864/j.issn.0578-1752.2012.13.017

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Polymorphism of Exon10 of FSHR Gene and Its Relationship with Litter Size in Xiaomeishan Pigs

 WU  Jing-Sheng, WANG  Jin-Yu   

  1. 1.扬州大学动物科学与技术学院，江苏扬州 225009
    2.江苏规模化猪场高效健康养殖公共技术服务中心，江苏扬州 225009
    3.江苏农林职业技术学院，江苏句容 212400
  • Received:2011-08-17 Online:2012-07-01 Published:2012-01-12

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Abstract: 【Objective】The effect of polymorphism of exon10 of FSHR gene on litter size in Xiaomeishan pigs was studied.【Method】The polymorphism of exon10 of FSHR gene was investigated by PCR-SSCP and direct sequencing methods, and the effect of exon10 of FSHR gene on litter size traits in Xiaomeishan pigs was analyzed using the least square analysis. Simultaneously the variance components were analyzed and selective reaction was predicted.【Result】 There was one novel polymorphic locus dected by primers P1 and P3, respectively, and the former made amino acid change, among Xiaomeishan, Fengjing and Large White pigs. One polymorphic locus detected by primer P6 was only in Large White pigs. Allele A at locus P1 and allele D at P3 locus were the dominant alleles in Xiaomeishan pigs. AA genotypic sow of Xiaomeishan pigs produced 1.95 TNB (total number born) and 1.66 NBA (number born alive) more than BB genotypic sow at locus P1 after the second parity, respectively (P＜0.01). And DD genotypic sow of Xiaomeishan pigs produced more TNB and NBA than CC genotypic sow at locus P3 after the second parity and in all parities, respectively (P＜0.01). The heredity of TNB and NBA in Xiaomeishan pigs was mostly influenced by the genetic additive effect.【Conclusion】Allele A at locus P1 and allele D at locus P3 in exon10 of FSHR gene had significant effect on litter sizes in Xiaomeishan pigs.

Key words: FSHR gene, polymorphism, Xiaomeishan pigs, PCR-SSCP

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