Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (12): 2537-2543.doi: 10.3864/j.issn.0578-1752.2012.12.024

• RESEARCH NOTES • Previous Articles     Next Articles

Genetic Diversity of Saccharomyces cerevisiae Strains Revealed by Microsatellite Sequence Polymorphism

 FENG  Min, WANG  Chun-Xiao, LIU  Yan-Lin   

  1. 西北农林科技大学葡萄酒学院,陕西杨凌 712100
  • Received:2011-01-19 Online:2012-06-15 Published:2012-04-18

Abstract: 【Objective】A total of 48 Saccharomyces cerevisiae strains from Xinjiang and Ningxia regions were typed by 4 microsatellite markers, and the genetic relations among the strains were also analyzed.【Method】A total of 48 Saccharomyces cerevisiae strains were discriminated for polymorphism at the 4 selected loci: SCAAT1, SCYOR267c, C11, and YPL009c.【Result】A total of 87 alleles, which generated 39 genotypes, were found. The polymorphism information contents (PIC) of all strains at 4 loci were 0.8593-0.9115, suggesting that the 4 SSR loci were hypervariable. The heterozygosity expected and heterozygosity observed were 0.8803-0.9268 and 0.8333-0.9792, respectively.【Conclusion】The results showed the diversity of Saccharomyces cerevisiae strains in Ningxia and Xinjiang regions.

Key words: Saccharomyces cerevisiae, microsatellite markers, strain typing, biodiversity

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