Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (4): 625-632.doi: 10.3864/j.issn.0578-1752.2012.04.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning of MaACT3 Promoter from Mulberry (Morus alba) and Agrobacterium-Mediated Transient Expression in Leaves

 LI  Jun, ZHAO  Ai-Chun, WANG  Qian-Ling, ZHU  Juan-Juan, ZHANG  Qiong-Yu, JIN  Xiao-Yun, LI  Zhen-Gang, LU  Cheng, WU  Cun-Rong, YU  Mao-De   

  1. 1.西南大学生物技术学院/家蚕基因组生物学国家重点实验室，重庆 400715
  • Received:2011-06-14 Online:2012-02-15 Published:2011-09-08

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Abstract: 【Objective】 The objective of this study is to clone the promoter of MaACT3 from mulberry (Morus alba). 【Method】The promoter of MaACT3 was obtained by suppression PCR method and the activity of promoter was valued by agrobacterium-mediated transient expression. 【Result】 The sequence analysis indicated that exon I, intron I and partial exon II were included and the partial exon II was the same as mRNA of MaACT3 reported before. Mature leaves were used for agrobacterium-mediated transient expression. Green fluorescence was measured by fluorescence microscope and mRNA was also detected.【Conclusion】 The promoter of MaACT3 from mulberry expressed promoter activity and could be used in transgenic research of mulberry.

Key words: mulberry, adaptor, promoter, EGFP

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