Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (2): 208-217.doi: 10.3864/j.issn.0578-1752.2012.02.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

RFLP Analysis of Mitochondrial Genomes Between Cytoplasmic Male Sterile Line and Maintainer Line in Upland Cotton

 ZHANG  Xiao, ZHANG  Rui, SHI  Ji, MENG  Zhi-Gang, SUN  Guo-Qing, ZHOU  Tao, GUO  San-Dui   

  1. 1.中国农业科学院生物技术研究所/国家农作物基因资源与遗传改良重大科学工程，北京 100081
    2.长春理工大学生命科学技术学院，长春 130022
  • Received:2011-07-06 Online:2012-01-15 Published:2011-11-02

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Abstract: 【Objective】 Identification of the differences of mitochondrial genomes between cytoplasmic male sterile (CMS) line and maintainer line of cotton could make a foundation for cloning CMS-associated gene in cotton.【Method】In this study, 20 mitochondrial gene probes, atpA, atp6, atp9, ccmB, ccmC, ccmFN1, cob, coxI, coxII, coxIII, matR, nad1bc, nad2, nad4, nad5, nad6, nad7c, nad9, rpl5, and rrn18, were used to identify the differences between the P30B normal fertile (N-) and the P30A male sterile (S-) cytoplasm with Southern blot method.【Result】Among the 20 genes, atpA, atp9, ccmB, nad1bc, nad6, nad7c and rrn18 revealed restriction fragment length polymorphisms (RFLPs) between N- and S-cytoplasm. The differences in atpA locus were most obvious.【Conclusion】 atpA was an important mitochondrial genomic site that showed an obvious difference between CMS line and maintainer line of cotton. One rrn18 gene copy was deleted in the mitochondrial genome of CMS line.

Key words: cotton (Gossypium hirsutum L.), CMS (cytoplasmic male sterility), mitochondrial genome, RFLP, atpA

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