小麦TaDREB6转录因子互作蛋白的筛选

doi:10.3864/j.issn.0578-1752.2011.22.023

Abstract

Abstract: 【Objective】TaDREB6 was used as a bait to screen the interaction proteins from wheat cDNA library by yeast-two hybrid system to further explore the DREB-mediated stress resistance mechanism in wheat.【Method】The TaDREB6 gene was amplified using wheat cDNA as the template. The wheat cDNA library and bait vector pGBKT7-TaDREB6 were constructed, respectively. The mixture of bait vectors pGBKT7-TaDREB6, pGADT7 and wheat cDNA library was introduced into yeast competent cells AH109 and cultured on SD/-Ade/-His/-Leu/-Trp plates for incubation at 30℃ for 3-5 days. Then the clones whose diameter was greater than 2 mm were selected for incubation on the plates containing X-α-gal to screen blue clones.【Result】A total of 102 candidate positive clones were sequenced and analyzed through homology analysis using the BLAST in NCBI. Homology analysis showed that those candidate proteins were related to energy metabolism, stress and defense, transport, transcription and translation, signal transduction, growth and development.【Conclusion】Function prediction of the candidate proteins suggested that TaDREB6 was possibly involved in several stress signal transduction pathways and play a important role in regulation stress resistance.

Key words: common wheat, yeast two-hybrid, DREB, interaction protein, stress-resistant mechanism

SUN Hai-Tao, XU Zhao-Shi, HOU Jian-Hua, YU Zhuo, ZHAO Yue, LI Lian-Cheng, CHEN Ming, MA You-Zhi. Screening of the Interaction Proteins of TaDREB6 Transcription Factor in Wheat[J].Scientia Agricultura Sinica, 2011, 44(22): 4740-4747.

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Screening of the Interaction Proteins of TaDREB6 Transcription Factor in Wheat

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