Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (22): 4669-4677.doi: 10.3864/j.issn.0578-1752.2011.22.014

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

An Indirect and Accurate ELISA for Detection of Alicyclobacillus acidoterrestris in Apple Juice Concentrate

 LI  Jian-Ke, WANG  Feng, XIA  Kai   

  1. 1.陕西师范大学食品工程与营养科学学院，西安 710062
    2.西安市产品质量监督检验所，西安 710075
  • Received:2011-03-29 Online:2011-11-15 Published:2011-08-23

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Abstract: 【Objective】 An establishment of a rapid and accurate indirect ELISA for detection of Alicyclobacillus acidoterrestris in apple juice concentrate(AJC) was studied. 【Method】 Polyclonal antibody was generated by immunizing rabbits with A. acidoterrestris separated from AJC and indirect enzyme-linked immunosorbent assay was performed to analyze the antigenicity of A. acidoterrestris and its spores. 【Result】 The new indirect ELISA was used successfully in detecting A. acidoterrestris for the first time, and the detection limit was 5×104 CFU/mL. In the detection of AJC, the samples were filter sterilized and enriched, followed by the indirect ELISA assay. This method could be performed in less than 24 h, and the minimum detection limit has reached the international standard for detecting A. acidoterrestris, which AJC is less than 1 CFU/10 mL. The result gained by indirect ELISA was in accord with that by KFL, a conventional culture assay. 【Conclusion】 The developed indirect ELISA has good specificity, sensitivity and reproducibility for the detection of A. acidoterrestris in juice concentrate, which is accurate, convenient, and quick.

Key words: apple juice concentrate, A. acidoterrestris, enzyme-linked immunosorbent assay, rapid detection

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