Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (19): 3971-3979.doi: 10.3864/j.issn.0578-1752.2012.19.008

• PLANT PROTECTION • Previous Articles     Next Articles

Rapid Detection and Identification of Fusarium oxysporum f. sp. cubense Race 1 and Race 4

 LI  Min-Hui, YU  Xiong-Tao, WANG  Hong-Fei, ZHOU  Jia-Nuan, XI  Ping-Gen, JIANG  Zi-De   

  1. 1.华南农业大学资源环境学院,广州 510640
    2.广东粤微食用菌技术有限公司, 广州 510663
  • Received:2012-02-07 Online:2012-10-01 Published:2012-06-21

Abstract: 【Objective】The objective of this study is to establish a method of rapid detection and identification of Fusarium oxysporum f. sp. cubense (FOC) race 1 and race 4 based on their respective specific sequence, avoid spreading and control banana Fusarium wilt, and further to provide a scientific basis for production and banana cultivar configuration. 【Method】DNAs of 6 FOC race 1 isolates, 7 FOC race 4 isolates, 7 F. oxysporum isolates and 2 outgrouped isolates were used as templates to screen the specific primers designed on the basis of candidate pathogenicity-relative gene sequences of FOC race 4 by PCR. 【Result】Two pairs of primers specific to FOC race 1 and race 4 respectively and one pair of universal primers of F. oxysporum were screened. Using the specific primers, pathogenic fungal DNA and diseased banana plant DNA and even contaminated soil DNA with the pathogen could be detected and identified. Meanwhile, the universal primers of F. oxysporum could also be used as control to test the DNA quality and avoid producing false negative and further to ensure the detected result. 【Conclusion】A rapid and accurate triple PCR detection method was established to identify FOC race 1 and race 4 in one PCR reaction, which is very useful to detect FOC races from banana tissue cultured plantlets and filed soil.

Key words: Fusarium oxysporum f. sp. cubense, race 1 and race 4, triple PCR, rapid detection and identification

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