Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (13): 2751-2757.doi: 10.3864/j.issn.0578-1752.2012.13.020

• VETERINARY SCIENCE • Previous Articles     Next Articles

Rapid Detection of Campylobacter jejuni Using Loop-Mediated Isothermal Amplification Assay

 XU  Yi-Gang, LI  Dan-Dan, TIAN  Chang-Yong, LIU  Zhong-Mei, LI  Su-Long, ZHANG  Guo-Cai   

  1. 1.黑龙江出入境检验检疫局检验检疫技术中心，哈尔滨 150001
    2.东北林业大学，哈尔滨 150040
    3.海南出入境检验检疫局检验检疫技术中心，海口 570125
    4.辽宁农业职业技术学院，辽宁营口 115009
  • Received:2011-07-29 Online:2012-07-01 Published:2012-06-01

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Abstract: 【Objective】Campylobacter jejuni is a major zoonotic pathogen, mainly initiates its transmission with contaminated water, milk products and raw poultry. Thus, the development of a rapid and accurate diagnostic method is an important measure to prevent and control C. jejuni.【Method】Loop-mediated isothermal amplification (LAMP) assay was introduced to develop a rapid method for the detection of C. jejuni using the ORF-C sequence of C. jejuni as the target gene.【Result】Twenty-one bacterial strains were used to evaluate the specificity of the LAMP method. The result showed that only C. jejuni was positive and the other strains were negative in LAMP, indicating that the LAMP method was highly specific to the target pathogen with the sensitivity of 5CFU per test tube for C. jejuni cultivated and 9 CFU per test tube for its contaminated materials.【Conclusion】The LAMP method developed in this work is a sensitive, rapid and simple tool for the detection of C. jejuni.   

Key words: Campylobacter jejuni, LAMP, rapid detection

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