Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (10): 1973-1979.doi: 10.3864/j.issn.0578-1752.2011.10.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Physiological Character and Gene Fine Mapping of a Virescent Mutant v13(t) in Rice (Oryza sativa L.)

 WANG  Jun, YANG  Jie, CHEN  Zhi-De, FAN  Fang-Jun, ZHU  Jin-Yan, YANG  Jin-Huan, ZHONG  Wei-Gong   

  1. 1.江苏省农业科学院粮食作物研究所/国家水稻改良中心南京分中心，南京 210014
  • Received:2010-10-21 Online:2011-05-15 Published:2011-01-13

PDF

1276

Cited

5

Abstract: 【Objective】On the basis of phenotypic characterizations of temperature sensitive virescent mutant v13(t), the gene virescent 13(t) was finely mapped. 【Method】 One natural virescent mutant, named virescent 13(t), was identified from the indica rice cultivar 9311. The physiological basis of the phenotypic variation was observed by transmission electron microscope. And a map-based cloning strategy was employed to reveal the genetic basis of this variation. 【Result】 At low temperature conditions (<26℃), the initial three leaves of v13(t) showed white, with little green tips and sheath, and the seedling gradually died. At 28℃, only the leaf tips and edge appeared white, and then the leaves turned green. While at 30℃, a significant difference between it and wild-type was not observed. To uncover the genetic basis of this variation, an F2 segregating population, v13(t) mutant, was developed by crossing v13(t) and Wuyunjing 7. Genetic analysis indicated that this leaf color variation was controlled by one recessive nuclear gene. Gene V13(t) was initially mapped between the SSR markers RM3638 and RM459 on chromosome 5, with the genetic distance of 3.2 cM and 0.5 cM, respectively. Several new InDel markers around the V13(t) region were further developed for fine mapping. The target gene was finally delimited to a 98.9 kb region between markers InDel5-11 and InDel5-8 on BAC AC130729, and segregated with marker InDel5-2. 【Conclusion】 One new temperature sensitive virescent mutant v13(t) was controlled by one recessive nuclear gene V13(t), and V13(t) was finally delimited to a 98.9 kb region on BAC AC130729.

Key words: rice (Oryza sativa L.), virescent gene, ultrastructure, fine mapping

[1]Oster U, Tanaka R, Tanaka A, Rüdier W. Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana. The Plant Journal, 2000, 21(3): 305-310.

[2]Jung K H, Hur J, Ryu C H, Choi W, Chung Y Y, Miyao A, Hirochika H, An G. Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system. Plant Cell Physiology, 2003, 44(5): 463-472.

[3]Babiychuk E, Müller F, Eubel H, Braun H P, Frentzen M, Kushnir S. Arabidopsis phosphatidylglycerophosphate synthase 1 is essential for chloroplast differentiation, but is dispensable for mitochondrial function. The Plant Journal, 2003, 33(5): 899-909.

[4]Schultes N P, Sawers R J, Brutnell T P, Krueger R W. Maize high chlorophyll fluorescent 60 mutation is caused by an Ac disruption of the gene encoding the chloroplast ribosomal small subunit protein 17. The Plant Journal, 2000, 21(4): 317-327.

[5]Motohashi R, Ito T, Kobayashi M, Taji T, Nagata N, Asami T, Yoshida S, Yamaguchi-Shinozaki K, Shinozaki K. Functional analysis of the 37 kDa inner envelope membrane polypeptide in chloroplast biogenesis using a Ds-tagged Arabidopsis pale-green mutant. The Plant Journal, 2003, 34(5): 719-731.

[6]Kurata N, Miyoshi K, Nonomura K I, Yamazaki Y, Ito Y. Rice mutants and genes related to organ development, morphogenesis and physiological traits. Plant Cell Physiology, 2005, 46(1): 48-62.

[7]Archer E K, Bonnett H T. Characterization of a virescent chloroplast mutant of tobacco. Plant Physiology, 1987, 83: 920-925.

[8]吴殿星, 舒庆尧, 夏英武, 郑  涛, 刘贵付. 一个新的水稻转绿型白化突变系W25的叶色特征及遗传. 浙江农业学报, 1996, 8(6): 372-374.

Wu D X, Shu Q Y, Xia Y W, Zheng T, Liu G F. Leaf color character and genetics of a new green able albino mutation line W25 of rice (Oryza sativa L.). Acta Agriculturae Zhejiangensis, 1996, 8(6): 372-374. (in Chinese)

[9]赵海军, 吴殿星, 舒庆尧, 沈圣泉, 马传喜. 携带白化转绿型叶色标记光温敏核不育系玉兔S的选育及其特征特性. 中国水稻科学, 2004, 18(6): 515-521.

Zhao H J, Wu D X, Shu Q Y, Shen S Q, Ma C X. Breeding and characteristics of photo-thermo sensitive genic male sterile rice Yutu S labeled with green-revertible albino leaf marker. Chinese Journal of Rice Science, 2004, 18(6): 515-521. (in Chinese)

[10]Chen T , Zhang Y D, Zhao L, Zhu Z, Lin J, Zhang S B, Wang C L. Physiological character and gene mapping in a new green-revertible albino mutant in rice. Journal of Genetics and Genomics, 2007, 34(4): 331-338.

[11]Chen T, Zhang Y D, Zhao L, Zhu Z, Lin J, Zhang S B, Wang C L. Fine mapping and candidate gene analysis of a green-revertible albino gene gra(t) in rice. Journal of Genetics and Genomics, 2009, 36(2): 117-123.

[12]兰  涛, 汪  斌, 凌秋平, 徐春花, 童治军, 梁康迳, 段远霖, 金  晶, 吴为人. 水稻苗期低温失绿基因cisc(t)的精细定位及其候选基因的确定. 科学通报, 2010, 55(22): 2183-2187.

Lan T, Wang B, Ling Q P, Xu C H, Tong Z J, Liang K J, Duan Y L, Jin J, Wu W R. Fine mapping of cisc(t), a gene for cold-induced seedling chlorosis, and identification of its candidate in rice. Chinese Science Bulletin, 2010, 55(22): 2183-2187. (in Chinese)

[13]Xia J C, Wang Y P, Ma B T, Yin Z Q , Hao M, Kong D W, Li S G. Ultrastructure and gene mapping of the albino mutant al12 in rice (Oryza sativa L.). Acta Genetica Sinica, 2006, 33(12): 1112-1119.

[14]李  超, 林冬枝, 董彦君, 叶胜海, 张小明. 一个水稻苗期温敏感白色条斑叶突变体的遗传分析及基因定位. 中国水稻科学, 2010, 24(3): 223-227.

Li C, Lin D Z, Dong Y J, Ye S H, Zhang X M. Genetic analysis and mapping of a thermo-sensitive white stripe leaf mutant at the seedling stage in rice (Oryza sativa L.). Chinese Journal of Rice Science, 2010, 24(3): 223-227. (in Chinese)

[15]Liang G H, Cao X Y, Sui J M, Zhao X Q, Yan C J, Yi C D, Gu M H. Fine mapping of a semidwarf gene sd-g in indica rice (Oryza sativa L.). Chinese Science Bulletin, 2004, 49(9): 900-904.

[16]Shen Y J, Jiang H, Jin J P, Zhang Z B, Xi B, He Y Y, Wang G, Wang C, Qian L, Li X, Yu Q B, Liu H J, Chen D H, Gao J H, Huang H, Shi T L, Yang Z N. Development of genome-wide DNA Polymorphism database for map-based cloning of rice genes. Plant Physiology, 2004, 135(3): 1198-1205.

[17]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the National Academy of Sciences of the USA, 1991, 88(21): 9828-9832.

[18]Lander E S, Green P, Abrahamson J. Mapmaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1: 174-181.

[19]Leister D. Chloroplast research in the genomic age. Trends in  Genetics, 2003, 19(1): 47-56.

[20]舒庆尧, 刘贵富, 夏英武. 温敏水稻叶色突变体的研究. 核农学报, 1996, 1(10): 6-10.

Shu Q Y, Liu G F, Xia Y W. Temperature-sensitve leaf color mutation in rice (Oryza sativa L.). Acta Agriculturae Nucleatae Sinica, 1996, 1(10): 6-10. (in Chinese)

[21]董彦君, 董文其, 张小明, 石守望, 张宏德. 突变体Fan5苗色低温敏感性状的遗传分析. 中国水稻科学, 1995, 4(9): 249-250.

Dong Y J, Dong W Q, Zhang X M, Shi S W, Zhang H D. Genetic analysis of low-temperature-sensitive seedling-colour character in the mutant Fan5. Chinese Journal of Rice Science, 1995, 4(9): 249-250. (in Chinese)

[22]吴殿星, 舒庆尧, 夏英武, 刘贵付, 黎军英. 水稻转绿型白化突变系W25的叶绿体超微结构研究. 浙江农业大学学报, 1997, 23(4): 451-452.

Wu D X, Shu Q Y, Xia Y W, Liu G F, Li J Y. Study on chloroplast uItrastructure of a greenable albine mutation line cv.W25 of rice (Oryza sativa). Journal of Zhejiang Agricultural University, 1997, 23(4): 451-452. (in Chinese)

[23]崔海瑞, 夏英武, 高明尉. 温度对水稻突变体W1叶色及叶绿素生物合成的影响. 核农学报, 2001, 15(5): 269-273.

Cui H R, Xia Y W, Gao M W. Effects of temperature on leaf color and chlorophyll biosynthesis of rice mutant W1. Acta Agriculturae Nucleatae Sinica, 2001, 15(5): 269-273. (in Chinese)

[24]Kusumi K, Mizutani A, Nishimura M, Iba K. A virescent gene V1 determines the expression timing of plastid genes for transcription/ translation apparatus during early leaf development in rice. The Plant Journal, 1997, 12(6): 1241-1250.

[25]Sugimoto H, Kusumi K, Tozawa Y, Yazaki J, Kishimoto N, Kikuchi S, Iba K. The virescent-2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation. Plant Cell Physiology, 2004, 45(8): 985-996.

[26]Yoo S C, Cho S H, Sugimoto H, Li J J, Kusumi K, Koh H J, Iba K, Paek N. Rice Virescent3 and Stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development. Plant Physiology, 2009, 150(1): 388-401.

[27]Satoh H, Iwata N, Omura T. Linkage analysis in rice. On the loci of new virescent genes, v-9(t) and v-10(t) and v-11(t). Japan Journal of Breeding, 1984, 34(Suppl.l): 286-287. (in Japanese)
[1] LI FeiFei, LIAN XueFei, YIN Tao, CHANG YuanYuan, JIN Yan, MA XiaoChuan, CHEN YueWen, YE Li, LI YunSong, LU XiaoPeng. The Relationship Between Mastication and Development of Segment Membranes in Citrus Fruits [J]. Scientia Agricultura Sinica, 2023, 56(2): 333-344.
[2] PENG Xue,GAO YueXia,ZHANG LinXuan,GAO ZhiQiang,REN YaMei. Effects of High-Energy Electron Beam Irradiation on Potato Storage Quality and Bud Eye Cell Ultrastructure [J]. Scientia Agricultura Sinica, 2022, 55(7): 1423-1432.
[3] ZHANG YaNan,JIN YongYan,ZHUANG ZhiWei,WANG Shuang,XIA WeiGuang,RUAN Dong,CHEN Wei,ZHENG ChunTian. Comparison of Shell Mechanical Property, Ultrastructure and Component Between Chicken and Duck Eggs [J]. Scientia Agricultura Sinica, 2022, 55(24): 4957-4968.
[4] MA LingLing,FENG Jia,WANG Jing,QI GuangHai,MA YouBiao,WU ShuGeng,ZHANG HaiJun,QIU Kai. The Changes of Eggshell Quality in the Laying Cycle of Hy-Line Brown Layers [J]. Scientia Agricultura Sinica, 2021, 54(17): 3766-3779.
[5] GAO YingBo,ZHANG Hui,SHAN Jing,XUE YanFang,QIAN Xin,DAI HongCui,LIU KaiChang,LI ZongXin. Effects of Pre-Silking High Temperature Stress on Yield and Ear Development Characteristics of Different Heat-Resistant Summer Maize Cultivars [J]. Scientia Agricultura Sinica, 2020, 53(19): 3954-3963.
[6] XU YunMei, LI YuMei, JIA YuXin, ZHANG ChunZhi, LI CanHui, HUANG SanWen, ZHU GuangTao. Fine Mapping and Candidate Genes Analysis for Regulatory Gene of Anthocyanin Synthesis in Red-Colored Tuber Flesh [J]. Scientia Agricultura Sinica, 2019, 52(15): 2678-2685.
[7] SUN CanRan, ZHANG XueHai, MA ZhiHui, GUO ZhanYong, TANG JiHua, FU ZhiYuan. Fine Mapping of Grain Test Weight Gene tw1 in Maize [J]. Scientia Agricultura Sinica, 2018, 51(7): 1233-1243.
[8] WANG BeiFang, CHEN YuYu, ZHANG YingXin, LIU QunEn, SUN Bin, XIANG XiaoJiao, CAO YongRun, CHENG ShiHua, CAO LiYong. Identification and Fine Mapping of an Early Senescent Leaf Mutant es5 in Oryza sativa L. [J]. Scientia Agricultura Sinica, 2018, 51(4): 613-625.
[9] YU YanFang, LIU Xi, TIAN YunLu, LIU ShiJia, CHEN LiangMing, ZHU JianPing, WANG YunLong, JIANG Ling, ZHANG WenWei, WANG YiHua, WAN JianMin. Phenotypic Analysis and Gene Mapping of a Floury and Shrunken Endosperm Mutant fse3 in Rice [J]. Scientia Agricultura Sinica, 2018, 51(11): 2023-2037.
[10] LU ZhenHua, NIU Liang, ZHANG NanNan, YAO JiaLong, CUI GuoChao, ZENG WenFang, PAN Lei, WANG ZhiQiang. Fine Mapping of Dwarfing Gene for Peach Based on SNP Markers [J]. Scientia Agricultura Sinica, 2017, 50(18): 3572-3580.
[11] SHAO Rui-xin, LI Lei-lei, ZHENG Hui-fang, XIN Long-fei, SU Xiao-yu, RAN Wu-ling, YANG Qing-hua. Effects of Exogenous Nitric Oxide on Photosynthesis of Maize Seedlings Under Drought Stress [J]. Scientia Agricultura Sinica, 2016, 49(2): 251-259.
[12] LIU Zi-gang, SUN Wan-cang, FANG Yan, LI Xue-cai, YANG Ning-ning, WU Jun-yan, ZENG Xiu-cun, WANG Yue. Effects of Low Nocturnal Temperature on Photosynthetic Apparatus of Winter Rapeseed (Brassica campestris L.) [J]. Scientia Agricultura Sinica, 2015, 48(4): 672-682.
[13] SHI Yan-xia, XU Yu-fang, XIE Xue-wen, CHAI A-li, WANG Wei-wei, LI Bao-ju. Effects of FBT on Induction of Systemic Resistance in Cucumber Against Cucumber Fusarium Wilt Caused by Fusarium oxysporum f. sp. cucumerinum Owen [J]. Scientia Agricultura Sinica, 2015, 48(19): 3848-3856.
[14] LIU Chen, KONG Wei-yi, YOU Shi-min, ZHONG Xiu-juan, JIANG Ling, ZHAO Zhi-gang, WAN Jian-min. Genetic Analysis and Fine Mapping of a Novel Rolled Leaf Gene in Rice [J]. Scientia Agricultura Sinica, 2015, 48(13): 2487-2496.
[15] ZHANG Xing-yuan, LUO Sheng, WANG Min, CONG Nan, ZHAO Zhi-chao, CHENG Zhi-jun. Fine Mapping of Rice Panicle Apical Abortion Gene qPAA3 Interacting with SP1 [J]. Scientia Agricultura Sinica, 2015, 48(12): 2287-2295.
Viewed
Full text
606
HTML PDF
Just accepted Online first Issue Just accepted Online first Issue
0 0 0 0 0 606

  From Others local
  Times 207 399
  Rate 34% 66%

Abstract
180
Just accepted Online first Issue
0 0 180
  From Others local
  Times 168 12
  Rate 93% 7%

Cited
Web of Science  Crossref   ScienceDirect  Search for Citations in Google Scholar >>
 
This page requires you have already subscribed to WoS.
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd