Abstract:

【Objective】 Elite cross combinations were bred using highly-male-sterile line with high outcrossing rate and herbicide-resistant restorer line. By this, two-line system hybrid millet could be used in millet production. 【Method】 Improved existing male sterile lines and restorer lines which suited breeding aim were bred by pedigree method. 【Result】 Four highly-male-sterile-lines with high outcrossing rate and fine agronomic traits named Gao117,Gao146,Gao229, and Gao236 were selected. Herbicide-resistant restorer lines with high combining ability like K103, etc.were bred. A set of technical system of breeding two-line system hybrid was found. The first millet hybrid named Changzagu No.2 with herbicide-resistance was bred which suits to late maturing area in China. 【Conclusion】 The puzzle about high-yielding reproduction of male sterile lines and high-yielding seed reproduction of hybrid were solved by having highly-male-sterile lines with high- outcrossing rate.

Key words: highly-male-sterile line, outcrossing rate, herbicide-resistant, combining ability