普通小麦Arableu#1白粉病成株抗性遗传解析

doi:10.3864/j.issn.0578-1752.2024.01.005

中国农业科学 ›› 2024, Vol. 57 ›› Issue (1): 52-64.doi: 10.3864/j.issn.0578-1752.2024.01.005

• 专题：小麦抗病新基因挖掘及品种培育 • 上一篇下一篇

普通小麦Arableu#1白粉病成株抗性遗传解析

李子萌¹(), 袁婵¹(), 张宇庆², 任妍³, 刘鹏鹏⁴, 严珊珊¹, 袭梦涵¹, 穆培源⁴, 兰彩霞¹()

¹ 华中农业大学植物科学技术学院/湖北洪山实验室，武汉 430070
² 湖北省农业科学院粮食作物研究所，武汉 430072
³ 河南农业大学农学院，郑州 450002
⁴ 新疆农垦科学院作物研究所，新疆石河子 832000

收稿日期:2023-07-07 接受日期:2023-09-05 出版日期:2024-01-01 发布日期:2024-01-10
通信作者:
兰彩霞，E-mail：cxlan@mail.hzau.edu.cn
联系方式: 李子萌，E-mail：18206462811@163.com。袁婵，E-mail：yuanchan9606@163.com。李子萌和袁婵为同等贡献作者。
基金资助:
湖北洪山实验培育项目(2022hspy001); 国家自然科学基金国际合作项目(31861143010)

Genetic Analysis of Adult Plant Resistance to Powdery Mildew in Common Wheat Arableu#1

LI ZiMeng¹(), YUAN Chan¹(), ZHANG YuQing², REN Yan³, LIU PengPeng⁴, YAN ShanShan¹, XI MengHan¹, MU PeiYuan⁴, LAN CaiXia¹()

¹ College of Plant Science and Technology, Huazhong Agricultural University/Hubei Hongshan Laboratory, Wuhan 430070
² Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430072
³ College of Agronomy, Henan Agricultural University, Zhengzhou 450002
⁴ Institute of Crop Research, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, Xinjiang

Received:2023-07-07 Accepted:2023-09-05 Published:2024-01-01 Online:2024-01-10

摘要/Abstract

摘要：

【目的】中国是世界上最重要的小麦生产国和消费国之一，确保小麦产量稳定对我国粮食安全具有重要意义。小麦白粉病是限制小麦产量的重要因素之一。选育抗病品种是控制该病害最经济、有效环保的途径，挖掘新成株抗性基因/位点为选育持久抗病小麦品种提供依据。【方法】由国际玉米小麦改良中心（CIMMYT）培育的普通小麦品系Arableu#1在苗期对白粉病表现为感病，而成株期呈现高水平抗性。为深入解析其抗病遗传机制，利用Arableu#1与高感品系Apav#1杂交，构建一个含有142个家系的F₅重组自交系群体，采用基因分型测序（genotyping-by-sequencing，GBS）平台测序，获得4 298个在亲本间具有多态性的分子标记，并进行群体检测，所获基因型用于构建整个群体的遗传连锁图。【结果】结合白粉病连续2年田间表型和基因型数据，使用IciMapping 4.1对田间病害严重度、病程曲线下面积（AUDPC）和病害多年平均严重度（MFDS）等性状进行QTL定位分析，在该群体中定位了4个来自Arableu#1的白粉病成株抗性位点，分别位于染色体1BL、5BL、6BS和7BL上，以及2个来自Apav#1的抗性位点，分别位于染色体2DS和4BL上，可解释白粉病表型变异介于3.1%—28.5%。6个位点聚合时的群体平均病害严重度达到各位点组合中的最低水平，QPm.hzau-1BL、QPm.hzau-4BL、QPm.hzau-5BL和QPm.hzau-7BL 4个位点聚合后的效应仅次于6个位点同时存在。【结论】将2个新位点QPm.hzau-5BL和QPm.hzau-7BL的两翼分子标记开发成KASP标记，经对570份世界各地育成品种或地方品种检测，2个位点单独存在或聚合在一起时均表现出不同程度的白粉病抗性，且这两个位点在我国小麦材料中的分布频率较CIMMYT的小麦种质中低。获得1个可能同时聚合6个白粉病抗性位点家系，其田间白粉病抗性可达近免疫水平。

关键词: 小麦, 白粉病, 成株抗性, 数量性状, 基因定位

Abstract:

【Objective】China is a significant wheat producer and consumer in the world. It is very important for our food security to making sure the stable wheat production. Wheat powdery mildew is an important leaf disease to affect the global wheat yield. Breeding resistant wheat varieties is one of the most economically and environmentally effective way to manage this disease. Identification of new adult plant resistant loci is very important for breeders to develop durable powdery mildew-resistant wheat varieties. 【Method】The CIMMYT-derived common wheat (Triticum aestivum L.) Arableu#1, showed susceptibility to powdery mildew at the seedling stage, but exhibited a high level of resistance to this disease at the adult plant stage in the field. In order to understand the genetic basis of powdery mildew resistance in this line, we made an F₅ recombinant inbred line (RIL) population between Arableu#1 and the susceptible line Apav#1 and genotyped by genotyping-by-sequencing (GBS) platform. We obtained 4 298 polymorphic molecular markers between the parents and analyze the 142 RILs. Joinmap 4.1 and IciMapping 4.1 were respectively used to construct the linage maps and identify the powdery mildew resistance QTL in this study. 【Result】Four adult plant resistance loci from Arableu#1 were identified on wheat chromosome arms 1BL, 5BL, 6BS and 7BL, while two loci from Apav#1 on chromosomes 2DS and 4BL, which can explain the powdery mildew disease severity variation from 3.1% to 28.5%. The MFDS of RIL reached the lowest level when it carried all six detected resistance loci, while RILs combing QPm.hzau-1BL, QPm.hzau-4BL, QPm.hzau-5BL, and QPm.hzau-7BL also showed lower disease severity over multiple environments. 【Conclusion】KASP markers KASP-5B-1 and KASP-7B-2 for QPm.hzau-5BL and QPm.hzau-7BL, respectively, were developed in the present study. The two loci showed varying degrees of resistance to powdery mildew when present individually or in combination based on the genotype and phenotype of 570 wheat germplasm collections. Additionally, the frequency of these two loci in Chinese wheat materials is lower compared to CIMMYT's wheat germplasm. A line combining 6 QTLs was found and it showed near-immune resistance to powdery mildew in the field. This material will be an important germplasm resource for breeders to develop powdery mildew-resistant wheat varieties.

Key words: wheat, powdery mildew, APR, quantitative traits, genetic mapping

李子萌, 袁婵, 张宇庆, 任妍, 刘鹏鹏, 严珊珊, 袭梦涵, 穆培源, 兰彩霞. 普通小麦Arableu#1白粉病成株抗性遗传解析[J]. 中国农业科学, 2024, 57(1): 52-64.

LI ZiMeng, YUAN Chan, ZHANG YuQing, REN Yan, LIU PengPeng, YAN ShanShan, XI MengHan, MU PeiYuan, LAN CaiXia. Genetic Analysis of Adult Plant Resistance to Powdery Mildew in Common Wheat Arableu#1[J]. Scientia Agricultura Sinica, 2024, 57(1): 52-64.

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链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2024.01.005

https://www.chinaagrisci.com/CN/Y2024/V57/I1/52

图/表 7

图1

图2

表1

Arableu#1×Apav#1重组自交系群体抗白粉病成株抗性QTL检测"

数量性状位点 QTL	性状 Traits	遗传位置 Genitic position (bp)	左标记 Left marker	右标记 Right marker	物理位置 Physical position (bp)	阈值 LOD	表型变异贡献率 PVE (%)	加性效应 Add^a
QPm.hzau- 1BL	PM2020HNAU^b	82	5411162\|F\|0-35:A>C	1132278\|F\|0-20:C>T	669241198-673742433	10.1189	17.0074	108.7386
	PMM^c	82	5411162\|F\|0-35:A>C	1132278\|F\|0-20:C>T	669241198-673742433	16.3082	19.3908	5.3090
	PM2020HNa^d	89	4005037	5324108\|F\|0-18:A>G	669241098-669316590	14.0842	22.0558	10.5464
	PM2019HNb	100	1112007	100041322\|F\|0-38:A>G	674392512-675401897	20.6815	19.1550	10.0301
	PM2019HNa	101	100041322\|F\|0-38:A>G	100084210\|F\|0-16:A>G	675401829-680220849	15.7207	22.9672	7.2837
	PM2019HNAU	102	100041322\|F\|0-38:A>G	100084210\|F\|0-16:A>G	675401829-680220849	17.5926	18.2297	98.5048
QPm.hzau- 5BL	PMM	101	1091498\|F\|0-37:C>T	1125898\|F\|0-46:T>C	571213585-571321896	3.4217	12.6502	3.5605
	PM2019HNa	105	4990348	4992023\|F\|0-12:C>T	564159095-565507647	4.1067	12.4673	4.9577
	PM2019HNb	105	4990348	4992023\|F\|0-12:C>T	564159095-565507647	4.0420	12.2839	6.5241
	PM2019HNAU	105	4990348	4992023\|F\|0-12:C>T	564159095-565507647	4.4736	13.5031	68.8906
QPm.hzau- 6BS	PM2019HNAU	181	1019642\|F\|0-23:A>G	2275142	291067175-346802356	6.6477	5.582	54.3958
	PM2019HNb	185	3021981	3027047\|F\|0-12:T>C	249498029-250762293	16.6672	14.599	9.4876
	PM2019HNa	186	3027047\|F\|0-12:T>C	3958061	249498029-271823336	5.3738	6.5224	3.9575
QPm.hzau- 7BL	PM2019HNa	447	3023211\|F\|0-16:G>T	1048655	750120578	6.7070	7.042	4.1106
	PM2019HNb	447	3023211\|F\|0-16:G>T	1048655	750120578	13.3810	19.306	9.4272
	PM2019HNAU	447	3023211\|F\|0-16:G>T	1048655	750120578	18.5323	13.6867	89.1064
	PM2020AU	456	1110896	2277387	744194196-739539181	4.7959	6.5277	62.9146
QPm.hzau- 2DS	PM2020HNa	40	100077941\|F\|0-49:C>G	1093454\|F\|0-34:G>A	22163592-22160735	4.7886	7.7274	-6.5343
QPm.hzau- 2DS	PM2019HNb	59	4910771\|F\|0-64:A>G	1112583	22290086-391305793	4.4180	3.0742	-4.6587
QPm.hzau- 4BL	PM2019HNb	88	4989722\|F\|0-15:T>C	1231372	526935193-541900628	12.5201	9.9972	-8.3699
	PM2020HNb	88	4989722\|F\|0-15:T>C	1231372	526935193-541900628	9.9310	17.9438	-8.3873
	PM2020HNAU	88	4989722\|F\|0-15:T>C	1231372	526935193-541900628	14.7970	28.5337	-165.1792
	PMM	88	4989722\|F\|0-15:T>C	1231372	526935193-541900628	14.3437	17.1539	-5.8169

表1

图3

表2

Arableu#1中成株抗白粉病位点效应以及多位点互作分析"

源 Source	家系个数 No. of RILs	平均病害严重度 MFDS	Ⅲ型SS Type Ⅲ SS	均方 Mean square	F 值 F value	Pr>F
YEAR			465.64513	465.64513	2.77	0.0972
None	3	65.42
QPm.hzau-2DS	3	61.25	1666.98	1666.98	9.93	0.00
QPm.hzau-4BL	2	60.35	13073.84	13073.84	77.87	<.0001
QPm.hzau-7BL	2	53.75	5313.59	5313.59	31.65	<.0001
QPm.hzau-6BS	4	48.23	5111.97	5111.97	30.45	<.0001
QPm.hzau-5BL	4	43.75	4592.06	4592.06	27.35	<.0001
QPm.hzau-1BL	5	43.50	11287.23	11287.23	67.23	<.0001
QPm.hzau-1BL×QPm.hzau-2DS	4	43.00	17860.56	5953.52	43.67	<.0001
QPm.hzau-4BL×QPm.hzau-6BS	1	38.75	29892.48	14946.24	77.27	<.0001
QPm.hzau-1BL×QPm.hzau-2DS×QPm.hzau-5BL	5	34.13	1062.37	531.18	3.91	0.02
QPm.hzau-1BL×QPm.hzau-5BL	1	32.50	1195.99	1195.99	8.49	0.00
QPm.hzau-5BL×QPm.hzau-6BS×QPm.hzau-7BL	2	31.88	1956.69	1956.69	10.12	0.00
QPm.hzau-5BL×QPm.hzau-7BL	5	31.25	896.09	896.09	4.63	0.03
QPm.hzau-2DS×QPm.hzau-4BL×QPm.hzau-6BS	1	25.94	1959.85	979.93	5.07	0.01
QPm.hzau-1BL×QPm.hzau-2DS×QPm.hzau-6BS	12	23.34	962.25	481.13	3.54	0.03
QPm.hzau-1BL×QPm.hzau-4BL×QPm.hzau-6BS	2	20.31	1992.52	996.26	7.34	0.00
QPm.hzau-1BL×QPm.hzau-2DS×QPm.hzau-4BL×QPm.hzau-6BS	4	16.44	7944.25	1134.89	8.32	<.0001
QPm.hzau-2DS×QPm.hzau-4BL×QPm.hzau-5BL×QPm.hzau-7BL	2	14.38	2822.06	564.41	4.01	0.00
QPm.hzau-2DS×QPm.hzau-4BL×QPm.hzau-5BL	2	14.06	19505.16	3901.03	27.70	<.0001
QPm.hzau-2DS×QPm.hzau-4BL×QPm.hzau-5BL×QPm.hzau-6BS	2	13.75	2019.21	403.84	2.87	0.01
QPm.hzau-1BL×QPm.hzau-2DS×QPm.hzau-4BL×QPm.hzau-7BL	1	12.50	10420.55	1488.65	10.92	<.0001
QPm.hzau-1BL×QPm.hzau-5BL×QPm.hzau-6BS×QPm.hzau-7BL	2	12.50	10264.40	1283.05	9.11	<.0001
QPm.hzau-1BL×QPm.hzau-2DS×QPm.hzau-4BL×QPm.hzau-5BL	2	12.45	6065.47	866.50	6.36	<.0001
QPm.hzau-1BL×QPm.hzau-2DS×QPm.hzau-4BL	1	11.38	19394.83	4848.71	35.56	<.0001
QPm.hzau-1BL×QPm.hzau-4BL×QPm.hzau-5BL×QPm.hzau-7BL	1	7.50	839.91	419.96	3.09	0.05
QPm.hzau-1BL×QPm.hzau-2DS×QPm.hzau-4BL×QPm.hzau-5BL×QPm.hzau-6BS×QPm.hzau-7BL	1	4.20	14531.45	1096.72	8.37	<.0001

表2

图4

图5

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普通小麦Arableu#1白粉病成株抗性遗传解析

Genetic Analysis of Adult Plant Resistance to Powdery Mildew in Common Wheat Arableu#1

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