中国小麦抗条锈病基因育种利用现状与策略

doi:10.3864/j.issn.0578-1752.2024.01.004

中国农业科学 ›› 2024, Vol. 57 ›› Issue (1): 34-51.doi: 10.3864/j.issn.0578-1752.2024.01.004

• 专题：小麦抗病新基因挖掘及品种培育 • 上一篇下一篇

中国小麦抗条锈病基因育种利用现状与策略

刘志勇¹^,²^,³(), 张怀志¹^,², 白斌⁴, 李俊⁵, 黄林⁶, 徐智斌⁷, 陈永兴¹^,², 刘旭⁸, 曹廷杰⁹, 李淼淼¹, 陆平¹, 吴秋红¹, 董玲丽¹, 韩玉林¹⁰, 殷贵鸿¹¹, 胡卫国⁹, 王西成⁹, 赵虹⁹, 闫素红⁸, 杨兆生⁸, 畅志坚¹², 王涛⁷(), 杨武云⁵(), 刘登才⁶(), 李洪杰¹³(), 杜久元⁴()

¹ 中国科学院遗传与发育生物学研究所/植物细胞与染色体工程国家重点实验室/种子创新研究院，北京 100101
² 中国科学院大学现代农业科学学院，北京 100049
³ 海南崖州湾种子实验室，海南三亚 572024
⁴ 甘肃省农业科学院小麦研究所，兰州 730070
⁵ 四川省农业科学院作物研究所，成都 610066
⁶ 四川农业大学小麦研究所，四川温江 611130
⁷ 中国科学院成都生物研究所，成都 610041
⁸ 中国农业科学院棉花研究所，河南安阳 455000
⁹ 河南省农业科学院小麦研究所，郑州 450002
¹⁰ 周口市农业科学院，河南周口 466001
¹¹ 河南农业大学农学院，郑州 450046
¹² 山西农业大学农学院/作物科学研究所，太原 030801
¹³ 中国农业科学院作物科学研究所，北京 100081

收稿日期:2022-07-25 接受日期:2022-09-06 出版日期:2024-01-01 发布日期:2024-01-10
通信作者:
刘志勇，E-mail：zyliu@genetics.ac.cn。
王涛，E-mail：wangtao@cib.ac.cn。
杨武云，E-mail：yangwuyun@126.com。
刘登才，E-mail：dcliu7@sicau.edu.cn。
李洪杰，E-mail：hongjie@caas.net.cn。
杜久元，E-mail：dujiuyuan1962@163.com
联系方式: 刘志勇，E-mail：zyliu@genetics.ac.cn。
基金资助:
中国科学院战略性先导科技专项(XDA24010305); 中国科学院科技服务网络计划(KFJ-STS-ZDTP-24)

Current Status and Strategies for Utilization of Stripe Rust Resistance Genes in Wheat Breeding Program of China

LIU ZhiYong¹^,²^,³(), ZHANG HuaiZhi¹^,², BAI Bin⁴, LI Jun⁵, HUANG Lin⁶, XU ZhiBin⁷, CHEN YongXing¹^,², LIU Xu⁸, CAO TingJie⁹, LI MiaoMiao¹, LU Ping¹, WU QiuHong¹, DONG LingLi¹, HAN YuLin¹⁰, YIN GuiHong¹¹, HU WeiGuo⁹, WANG XiCheng⁹, ZHAO Hong⁹, YAN SuHong⁸, YANG ZhaoSheng⁸, CHANG ZhiJian¹², WANG Tao⁷(), YANG WuYun⁵(), LIU DengCai⁶(), LI HongJie¹³(), DU JiuYuan⁴()

¹ Institute of Genetics and Developmental Biology, Chinese Academy of Sciences/State Key Laboratory of Plant Cell and Chromosome Engineering/The Innovative Academy of Seed Design, Beijing 100101
² College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049
³ Hainan Yazhou Bay Seed Laboratory, Sanya 572024, Hainan
⁴ Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070
⁵ Crop Research Institute, Sichuan Academy of Agriculture Sciences, Chengdu 610066
⁶ Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan
⁷ Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041
⁸ Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, Henan
⁹ Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002
¹⁰ Zhoukou Academy of Agricultural Sciences, Zhoukou 466001, Henan
¹¹ College of Agronomy, Henan Agricultural University, Zhengzhou 450046
¹² College of Agriculture, Shanxi Agricultural University/Institute of Crop Sciences, Taiyuan 030801
¹³ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081

Received:2022-07-25 Accepted:2022-09-06 Published:2024-01-01 Online:2024-01-10

摘要/Abstract

摘要：

小麦条锈病是中国重要的流行性真菌病害，严重威胁国家粮食安全。通过持续应用抗病品种和植保措施，小麦条锈病在中国得到了较好的控制。然而，随着全球气候变化、栽培制度的变革、育种方法技术的改进、产量水平的提高及条锈菌群体结构和毒性频率的不断变异，有必要对新时期小麦抗条锈病基因育种利用现状进行科学评估，以期为广谱持久多抗小麦品种的培育提供依据。本文通过对近年来中国西南、西北和黄淮主产区小麦品种（系）的条锈病抗性进行系统的抗病鉴定、遗传分析和抗条锈病基因分子标记的定位和检测，总结了中国小麦育种中主要抗条锈病基因的利用情况，对中国小麦抗条锈病基因发掘与种质创新、小麦育种中利用的主要抗条锈病基因、新时期抗条锈病基因利用的策略、小麦育种中抗条锈病基因选择和鉴定等进行了论述，并对新时期小麦抗条锈病育种发展方向进行了展望。

关键词: 小麦, 条锈菌, 兴衰循环, 持久抗性, 基因聚合, 种质创新

Abstract:

Wheat stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is a devastating disease threaten food security in China and worldwide. Epidemics of wheat stripe rust have been under control through applying resistant cultivars and crop protection approaches. However, due to climate change, innovation of cropping system, improvement of breeding technology, yield level enhancement of wheat cultivars, variation in structure and frequency of virulence genes in Pst populations in the new era, the current status of stripe rust resistance genes in wheat breeding programs need to be evaluated. The results could provide useful information for applying stripe rust resistance genes to develop new wheat cultivars with broad-spectrum and durable rust resistance. After multiple year’s stripe rust resistance survey, genetic analysis, molecular tagging and mining of stripe rust resistance genes in wheat cultivars and advanced breeding lines, the current status of major stripe rust resistance genes utilization was reviewed. We summarized the present situations of major stripe rust resistance gene discovery and germplasm innovation, the most frequently used stripe rust resistance genes, new strategy for pyramiding adult plant partial resistance and all stage resistance, and molecular marker assisted selection for developing wheat cultivars with broad spectrum and durable resistance in China. This review also proposes the major research areas in wheat stripe rust resistance breeding in the new era.

Key words: wheat, Puccinia striiformis f. sp. tritici, boom and burst cycle, durable resistance, gene pyramiding, germplasm innovation

刘志勇, 张怀志, 白斌, 李俊, 黄林, 徐智斌, 陈永兴, 刘旭, 曹廷杰, 李淼淼, 陆平, 吴秋红, 董玲丽, 韩玉林, 殷贵鸿, 胡卫国, 王西成, 赵虹, 闫素红, 杨兆生, 畅志坚, 王涛, 杨武云, 刘登才, 李洪杰, 杜久元. 中国小麦抗条锈病基因育种利用现状与策略[J]. 中国农业科学, 2024, 57(1): 34-51.

LIU ZhiYong, ZHANG HuaiZhi, BAI Bin, LI Jun, HUANG Lin, XU ZhiBin, CHEN YongXing, LIU Xu, CAO TingJie, LI MiaoMiao, LU Ping, WU QiuHong, DONG LingLi, HAN YuLin, YIN GuiHong, HU WeiGuo, WANG XiCheng, ZHAO Hong, YAN SuHong, YANG ZhaoSheng, CHANG ZhiJian, WANG Tao, YANG WuYun, LIU DengCai, LI HongJie, DU JiuYuan. Current Status and Strategies for Utilization of Stripe Rust Resistance Genes in Wheat Breeding Program of China[J]. Scientia Agricultura Sinica, 2024, 57(1): 34-51.

图/表 8

图1

表1

正式命名的小麦抗条锈病基因"

基因 Gene	位置 Location	基因来源 Origin	载体品种/系 Cultivar/line	抗病类型 Resistance type	编码蛋白 Encoded protein
Yr1	2AL	T. aestivum	Chinese 166	ASR
Yr2	7B	T. aestivum	Heines Ⅶ	ASR
Yr3a	5BL	T. aestivum	Cappelle Desprez	ASR
Yr3b	5BL	T. aestivum	Hybrid 46	ASR
Yr3c	5BL	T. aestivum	Minister	ASR
Yr4a	3B	T. aestivum	Cappelle Desprez	ASR
Yr4b	3B	T. aestivum	Hybrid 46	ASR
Yr5	2BL	T. spelta	Album	ASR	BED-NLR^[10]
Yr6	7BS	T. aestivum	Heines Kolben	ASR
Yr7	2BL	T. durum	Lee	ASR	BED-NLR^[10]
Yr8	2M/2D	Ae. comosa	Compair	ASR
Yr9	1RS	S. cereale	Clement	ASR
Yr10	1BS	T. aestivum	Moro	ASR	NAM-ZnF-BED^[11]
Yr11	/	T. aestivum	Joss Cambier	APR
Yr12	/	T. aestivum	Mega	APR
Yr13	/	T. aestivum	Maris Huntsman	APR
Yr14	/	T. aestivum	Hobbit	APR
Yr15	1BS	T. dicoccoides	G-25	ASR	Wheat tandem kinase 1^[12]
Yr16	2DL	T. aestivum	Capelle Desprez	APR
Yr17	2AS	Ae. ventricosa	VPM 1	ASR
Yr18	7DS	T. aestivum	Jupateco 73R	APR	ABC transporter^[13]
Yr19	5B	Ae. speltoids	Compair	ASR
Yr20	6D	T. aestivum	Fielder	ASR
Yr21	1BL	T. aestivum	Lemhi	ASR
Yr22	4D	T. aestivum	Lee	ASR
Yr23	6D	T. aestivum	Lee	ASR
Yr24	1BS	T. durum	K773	ASR
Yr25	1D	T. aestivum	Strubes Dickkopf	ASR
Yr26	1BL	T. turgidum	R55	ASR
Yr27	2BS	T. aestivum	Ciano79	ASR	NLR^[14]
Yr28	4DS	Ae. tauschii	W7984	ASR	NLR^[15]
Yr29	1BL	T. aestivum	Pavon76	APR
Yr30	3BS	T. aestivum	Opata85	APR
Yr31	2BS	T. aestivum	Pastor	ASR
Yr32	2AS	T. aestivum	Carstens V	ASR
Yr33	7DL	T. aestivum	Batavia	ASR
Yr34	5AL	T. aestivum	WAWHT 2046	APR
Yr35	6BS	T. dicoccoides	98M71	ASR
Yr36	6BS	T. dicoccoides	RSL65	HTAP	START-Kinase^[9]
Yr37	2DL	Ae. kotschyi	S14	ASR
Yr38	6AS	Ae. sharonenisis	Line 03524	ASR
Yr39	7BL	T. aestivum	Alpowa	HTAP
Yr40	5DS	Ae. geniculata	T5DL.5DS-5MgS	ASR
Yr41	2BS	T. aestivum	Chuannong 19	ASR
Yr42	6AL	Ae. neglecta	03M119-71A	ASR
Yr43	2BL	T. aestivum	IDO377s	ASR
Yr44	2BL	T. aestivum	Zak	ASR
Yr45	3DL	T. aestivum	PI 181434	ASR
Yr46	4DL	T. aestivum	RL 6007	APR	Hexose transporter^[16]
Yr47	5BS	T. aestivum	Aus 28183	ASR
Yr48	5AL	T. aestivum	PI610750	APR
Yr49	3DS	T. aestivum	Chuangmai 18	APR
Yr50	4BL	Th. Intermedium	CH223	ASR
Yr51	4AL	T. aestivum	Aus 27858	ASR
Yr52	7BL	T. aestivum	PI 183527	HTAP
Yr53	2BL	T. durum	PI 480148	ASR
Yr54	2DL	T. aestivum	Quaiu 3	APR
Yr55	2DL	T. aestivum	Aus 38882	ASR
Yr56	2AS	T. durum	Wollaroi	APR
Yr57	3BS	T. aestivum	Aus 27858	ASR
Yr58	3BS	T. aestivum	Aus 19292	APR
Yr59	7BL	T. aestivum	PI 178759	HTAP
Yr60	4AL	T. aestivum	Almop	APR
Yr61	7AS	T. aestivum	Pindong 34	ASR
Yr62	4BL	T. aestivum	PI 192252	HTAP
Yr63	7BS	T. aestivum	Aus 27955	/
Yr64	1BS	T. durum	PI 331260	ASR
Yr65	1BS	T. durum	PI 480016	ASR
Yr66	3DS	T. aestivum	MSP4543.1	ASR
Yr67	7BL	T. aestivum	AGG91585WHEA	ASR
Yr68	4BL	T. aestivum	AGG91587WHEA1	APR
Yr69	2AS	Th. Ponticum	CH 7086	ASR
Yr70	5DS	Ae. umbellulata	IL 393-4	ASR
Yr71	3DL	T. aestivum	Sunco	APR
Yr72	2BL	T. aestivum	Aus 27507	/
Yr73	3DL	T. aestivum	Avocet R	ASR
Yr74	5BL	T. aestivum	Avocet R	ASR
Yr75	7AL	T. aestivum	Nyabing-3	APR
Yr76	3AS	T. aestivum	Tyee	ASR
Yr77	6DS	T. aestivum	PI 322118	APR
Yr78	6BS	T. aestivum	PI 519805	APR
Yr79	7BL	T. aestivum	PI 182103	HTAP
Yr80	3BL	T. aestivum	Aus 27284	APR
Yr81	6AS	T. aestivum	Aus 27430	ASR
Yr82	3BL	T. aestivum	Aus 27969	ASR
Yr83	6RL	S. cereale	T-701	APR
Yr84	1BS	T. dicoccoides	PI 487260	ASR
Yr85	1BS	T. aestivum	Tres	ASR
Yr86	2AL	T. aestivum	Zhongmai 895	APR
YrU1	5AL	T. urartu	PI 428309	ASR	ANK-NLR-WRKY^[17]

表1

表2

不同麦区小麦育种中利用的主要抗条锈病基因"

区域 Region	抗条锈病基因 Yr gene	抗病类型 Resistance type	代表性载体品种 Representative cultivars
北部冬麦区 Northern China Winter Wheat Zone	Yr9	ASR	京冬8号、农大3291、兰天11、兰天132等 Jingdong 8, Nongda 3291, Lantian 11, Lantian 132, et al.
	Yr17	ASR	中麦175、轮选6号、兰天21、兰天132等 Zhongmai 175, Lunxuan 6, Lantian 21, Lantian 132, et al.
	Yr18/Lr34/Sr57/Pm38	APR	Libellula、Strampelli、武都17、天选44 Libellula, Strampelli, Wudu 17, Tianxuan 44
	Yr24/Yr26/YrCH42	ASR	兰天17、兰天30、兰天35等 Lantian 17, Lantian 30, Lantian 35, et al.
	Yr29/Lr46/Sr58/Pm39	APR	兰天27、兰航选151、天选50、长武357-9等 Lantian 27, Lanhangxuan 151, Tianxuan 50, Changwu 357-9, et al.
	Yr30/Lr27/Sr2/Pm70	APR	兰天15、兰天34、兰天132等 Lantian 15, Lantian 34, Lantian 132, et al.
	YrZH22	APR	兰天22、兰天33、兰天39、兰天44等 Lantian 22, Lantian 33, Lantian 39, Lantian 44, et al.
	YrZH84	ASR	兰天33、兰天36、兰天37等 Lantian 33, Lantian 36, Lantian 37, et al.
	YrZM175	ASR	中麦175 Zhongmai 175
黄淮麦区 Huang and Huai Rivers Winter Wheat Zone	YrJM22	ASR	济麦22 Jimai 22
	Yr9	ASR	农大399、石4185、04中36、矮抗58、周8425B等 Nongda 399, Shi 4185, 04zhong 36, Aikang 58, Zhou 8425B, et al.
	Yr17	ASR	孟麦58、淮阳1号、陕农33 Mengmai 58, Huaiyang 1, Shaannong 33
	Yr18/Lr34/Sr57/Pm38	APR	中国春、济麦22 Chinese Spring, Jimai 22
	Yr29/Lr46/Sr58/Pm39	APR	百农64、济南17、科农1006、西农528、陕农33 Bainong 64, Jinan 17, Kenong 1006, Xinong 528, Shaannong 33
	Yr30/Lr27/Sr2/Pm70	APR	周8425B、周麦22等 Zhou 8425B, Zhoumai 22, et al.
	YrZH22	APR	周8425B、周麦22等 Zhou 8425B, Zhoumai 22, et al.
	YrZH84	ASR	周8425B、周麦22等 Zhou 8425B, Zhoumai 22, et al.
	YrZY1152	ASR	中育1152 Zhongyu 1152
	YrZM103	ASR	郑麦103 Zhengmai 103
西南麦区 Southern West Winter Wheat Zone	Yr5	ASR	S2199
	Yr17	ASR	川麦1691、绵麦52、内麦416、川农41 Chuanmai 1691, Mianmai 52, Neimai 416, Chuannong 41
	Yr18/Lr34/Sr57/Pm38	APR	中国春、SY95-71、川麦801、川麦1456 Chinese Spring, SY95-71, Chuanmai 801, Chuanmai 1456
	Yr24/Yr26/YrCH42	ASR	贵农系列、92R系列和川麦42 Guinong series, 92R series and Chuanmai 42
	Yr29/Lr46/Sr58/Pm39	APR	SW8588、川麦1691、蜀麦830和蜀麦126 SW8588, Chuanmai 1691, Shumai 830 and Shumai 126
	Yr30/Lr27/Sr2/Pm70	APR	川麦88、西科麦12 Chuanmai 88, Xikemai 12
	Yr15	ASR	绵麦835、蜀麦1868、川育29和川辐8号 Mianmai 835, Shumai 1868, Chuanyu 29 and Chuanfu 8
	Yr28	ASR	蜀麦1675 Shumai 1675
	Yr36	APR	蜀麦1701 Shumai 1701
	Yr41	ASR	川农17 Chuannong 17
	Yr69	ASR	蜀麦126 Shumai 126

表2

图2

图3

图4

图5

图6

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中国小麦抗条锈病基因育种利用现状与策略

Current Status and Strategies for Utilization of Stripe Rust Resistance Genes in Wheat Breeding Program of China

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