中国农业科学 ›› 2024, Vol. 57 ›› Issue (9): 1674-1686.doi: 10.3864/j.issn.0578-1752.2024.09.005

• 专题:小麦抗旱性鉴定及基因资源挖掘 • 上一篇    下一篇

基于无人机多源影像数据的灌浆期人工合成小麦抗旱性评价

燕雯1,2(), 金秀良2, 李龙2, 徐子涵3, 苏悦1,2, 张跃强3, 景蕊莲2, 毛新国2(), 孙黛珍1()   

  1. 1 山西农业大学农学院,山西太谷 030801
    2 中国农业科学院作物科学研究所/作物基因资源与育种全国重点实验室,北京 100081
    3 新疆农业科学院核技术生物技术研究所/农业农村部荒漠绿洲区作物生理生态与耕作重点实验室/新疆作物生物技术重点实验室,乌鲁木齐 830091
  • 收稿日期:2024-01-05 接受日期:2024-03-11 出版日期:2024-05-01 发布日期:2024-05-09
  • 通信作者:
    孙黛珍,E-mail:
    毛新国,E-mail:
  • 联系方式: 燕雯,E-mail:yanwenzzl@163.com。
  • 基金资助:
    国家重点研发计划(2023YFD1201003); 国家重点研发计划(2022YFD1200201); 国家小麦产业技术体系(CARS-03-5)

Drought Resistance Evaluation of Synthetic Wheat at Grain Filling Using UAV-Based Multi-Source Imagery Data

YAN Wen1,2(), JIN XiuLiang2, LI Long2, XU ZiHan3, SU Yue1,2, ZHANG YueQiang3, JING RuiLian2, MAO XinGuo2(), SUN DaiZhen1()   

  1. 1 College of Agronomy, Shanxi Agricultural University, Taigu 030801, Shanxi
    2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/State Key Laboratory of Crop Gene Resources and Breeding, Beijing 100081
    3 Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Crop Ecophysiology and Farming System in Desert Oasis Region, Ministry of Agriculture and Rural Affairs/Xinjiang Key Laboratory of Crop Biotechnology, Urumqi 830091
  • Received:2024-01-05 Accepted:2024-03-11 Published:2024-05-01 Online:2024-05-09

摘要:

【目的】基于无人机多源影像及产量数据评价人工合成小麦种质的抗旱性,优选高通量抗旱性鉴定指标,发掘抗旱人工合成小麦种质资源,为加快拓展小麦抗旱遗传资源、提升旱地小麦育种水平提供技术支撑和种质材料。【方法】以80份人工合成小麦种质及对照小麦品种新春37为试验材料,在田间进行小区播种,设置干旱和灌溉2种水分处理;利用无人机搭载多光谱及热红外相机采集试验材料灌浆期多源影像进行拼接处理,通过阈值分割等方法提取各试验材料的光谱指数;利用相关性分析和主成分分析鉴选抗旱相关光谱指标,结合单指标及综合评价方法鉴定人工合成小麦种质的抗旱性。【结果】基于无人机多源影像数据提取了80份人工合成小麦种质的19种光谱指数。不同光谱指数抗旱系数与小区产量抗旱指数的相关性分析结果表明,OSAVI的抗旱系数与抗旱指数的关联度最高,NDVI、CIre和NDRE的抗旱系数与抗旱指数的关联度较高。部分光谱指数的抗旱系数间相关性较高,存在冗余信息,通过主成分分析,将19个光谱指数的抗旱系数转换为3个相互独立的综合指标,3个综合指标的贡献度分别为59.6%、12.0%和9.6%。利用加权隶属函数法聚合综合指标,通过公式计算获得各人工合成小麦种质的综合抗旱性度量值。基于抗旱指数鉴定出6份强抗旱人工合成小麦种质,基于综合抗旱性度量值鉴定出5份强抗旱种质,其中,SW004和SW009在2种方法的评价结果中均被评为强抗旱种质。基于OSAVI的抗旱系数对80份人工合成小麦种质进行抗旱性分级,分级结果与基于综合抗旱性度量值的分级结果基本一致。根据OSAVI的抗旱系数鉴定出的6份强抗旱种质中,有5份在基于综合抗旱性度量值分级中也被鉴定为强抗旱种质。【结论】基于无人机多源影像提取的光谱指数NDVI、OSAVI、CIre和NDRE,以及基于光谱指数的综合抗旱性度量值均可用于辅助鉴定小麦种质抗旱性。

关键词: 多源影像, 光谱指数, 人工合成小麦, 抗旱性, 灌浆期

Abstract:

【Objective】To evaluate the drought resistance of synthetic wheat germplasm based on multi-source images collected by unmanned aerial vehicle (UAV) and yield data, explore high-throughput indices for drought resistance evaluation, and identify synthetic wheat germplasm resources with drought resistance. This provides technical support and germplasm materials for accelerating the expansion of drought-resistant genetic resources for wheat and enhancing the level of breeding for dryland wheat.【Method】Eighty synthetic wheat germplasm and the control variety Xin Chun 37 were used as plant materials, which were sown in the field and treated with a water regime of drought stress and irrigation. Multi-source images of test materials during filling stage were collected by multi-spectral and thermal infrared cameras equipped with unmanned aerial vehicle, and the spectral index of each test material was extracted by threshold segmentation. The analyses of Pearson’s correlation and principal component were performed to identify drought resistance-related spectral indices, and the drought resistance of each synthetic wheat germplasm was determined by single index and comprehensive evaluation methods. 【Result】The drought resistance coefficients of 19 spectral indices of 80 synthetic wheat germplasm were calculated based on multisource imagery data obtained from unmanned aerial vehicles. The correlation analysis between the spectral indices and the yield-based drought index (DRI) showed that among the drought resistance coefficients of the 19 spectral indices, OSAVI exhibited the highest correlation with the drought index, while NDVI, CIre, and NDRE demonstrated relatively strong associations with the drought index. However, the different drought indices showed a high correlation, resulting in redundant information. The drought resistance coefficients of the 19 spectral indices were transformed into three independent comprehensive indicators through principal component analysis, with contribution rates of 59.6%, 12.0% and 9.6%, respectively. The comprehensive drought resistance index (D) for each synthetic wheat germplasm were calculated by aggregating the three independent comprehensive indicators using the weighted membership function method. 6 and 5 synthetic wheat germplasms with strong drought resistance were identified based on DRI and D, respectively. Among them, 2 germplasms (SW004 and SW009) with high drought resistance were detected based on both DRI and D. Furthermore, the drought resistance of the 80 synthetic wheat germplasms was graded based on the drought resistance coefficient of OSAVI, and the grading results were found to be consistent with that based on the D value. Among the six strongly drought-resistant germplasms identified based on the drought resistance coefficient of OSAVI, five of them were also classified as strongly drought-resistant germplasms based on comprehensive drought resistance evaluation.【Conclusion】The spectral indices NDVI, OSAVI, CIre and NDRE extracted from UAV-based multi-source images, as well as the drought resistance comprehensive evaluation value can be used to assist in the identification of drought resistance of wheat germplasm.

Key words: multi-source images, spectral index, synthetic wheat, drought resistance, grain filling