水稻分蘖期干物质积累对大气CO2浓度升高和氮素营养的综合响应差异及其生理机制

doi:10.3864/j.issn.0578-1752.2023.06.003

中国农业科学 ›› 2023, Vol. 56 ›› Issue (6): 1045-1060.doi: 10.3864/j.issn.0578-1752.2023.06.003

• 耕作栽培·生理生化·农业信息技术 • 上一篇下一篇

水稻分蘖期干物质积累对大气CO₂浓度升高和氮素营养的综合响应差异及其生理机制

贺江¹^,²(), 丁颖², 娄向弟², 姬东玲¹, 张向向², 王永慧², 张伟杨¹, 王志琴¹, 王伟露¹^,³(), 杨建昌¹

¹ 江苏省作物遗传生理重点实验室/江苏省作物栽培生理重点实验室/扬州大学农学院，江苏扬州 225009
² 江苏沿海地区农业科学研究所，江苏盐城 224002
³ 教育部农业与农产品安全国际合作联合实验室/扬州大学农业科技发展研究院，江苏扬州 225009

收稿日期:2022-06-28 接受日期:2022-08-02 出版日期:2023-03-16 发布日期:2023-03-23
联系方式: 贺江，E-mail：hejiang0323@163.com。
基金资助:
江苏省自然科学青年基金(BK20200923); 国家自然科学基金(32201888); 国家自然科学基金(32071943); 国家重点研发计划(SQ2022YFD2300304); 国家重点研发计划(2018YFD0300801); 江苏高校优势学科建设工程资助项目(PAPD); 扬州市“绿杨金凤”人才引进计划

Difference in the Comprehensive Response of Dry Matter Accumulation of Rice at Tillering Stage to Rising Atmospheric CO₂ Concentration and Nitrogen Nutrition and Its Physiological Mechanism

HE Jiang¹^,²(), DING Ying², LOU XiangDi², JI DongLing¹, ZHANG XiangXiang², WANG YongHui², ZHANG WeiYang¹, WANG ZhiQin¹, WANG WeiLu¹^,³(), YANG JianChang¹

¹ Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Agricultural College of Yangzhou University, Yangzhou 225009, Jiangsu
² Jiangsu Coastal Agricultural Science Research Institute, Yancheng 224002, Jiangsu
³ Joint International Research Laboratory of Agriculture and Agri-product Safety, Ministry of Education/Institutes of Agricultural Science and Technology Development of Yangzhou University, Yangzhou 225009, Jiangsu

Received:2022-06-28 Accepted:2022-08-02 Published:2023-03-16 Online:2023-03-23

摘要/Abstract

摘要：

【目的】探究不同类型水稻品种物质生产响应大气CO₂浓度升高和氮素营养的综合响应差异及其生理机制。【方法】以产量和物质生产对CO₂浓度升高响应有明显差异的水稻品种两优培九（LY）和南粳9108（NJ）为材料，在人工气候室进行水培试验。分别设置对照CO₂浓度（A-CO₂，400 μmol·mol^-1）和CO₂浓度升高（E-CO₂，600 μmol·mol^-1）两个CO₂处理，高氮（HN，1.25 mmol·L^-1 NH₄NO₃）和低氮（LN，0.25 mmol·L^-1 NH₄NO₃）两个氮水平。分析CO₂浓度升高对不同水稻品种根系形态与生理活性、叶片和根系中细胞分裂素（CTKs）含量、氮素同化酶活性、叶片生理特性、光合参数以及干物质积累的影响差异。【结果】（1）E-CO₂显著增加了LY总冠根数、总根长（LN水平除外）、总根表面积和平均直径，提高其根系呼吸速率和维持较高的根系氧化力，而对NJ无显著影响或表现相反；（2）无论氮水平如何，E-CO₂显著提高了LY叶片和根系CTKs含量，但显著降低了HN水平下NJ根系中玉米素核苷（ZR）含量；（3）在LN水平下，E-CO₂显著提高了LY叶片GOGAT、GDH活性，显著降低了NJ叶片NR活性。在HN水平下，LY氮同化酶活性在E-CO₂条件下都表现为提高，NJ仅NR活性提高；（4）在LN水平下，E-CO₂使得LY和NJ净光合速率（P_n）分别提高了28.0%和29.4%。在HN水平下，两品种分别提高了41.0%和28.1%。LY光合响应大幅度提高归因于叶片最大羧化效率（V_c,max）、最大光合电子传递效率（J _max）、核酮糖-1,5-二磷酸羧化酶（Rubisco）含量、叶绿素含量、叶片氮含量等显著提高；（5）E-CO₂显著增加了不同氮水平下LY单株叶面积，对NJ无显著影响；（6）E-CO₂显著增加了LY各器官及总生物量，且HN水平增幅明显大于LN水平。E-CO₂并未显著影响不同氮水平下NJ的总生物量，显著降低了HN水平下NJ地下部生物量（-16.7%）。【结论】无论在HN还是LN水平下，LY物质生产和生理特征对E-CO₂的响应幅度与NJ相比更高。生育前期LY较优的根系形态性状和根系活力、较高的CTKs含量、较强的氮素同化能力、较大的绿叶面积以及光合响应能力是其干物质生产对E-CO₂响应幅度较高的重要原因。

关键词: 水稻, CO₂浓度升高, 根系形态, 细胞分裂素, 光合作用, 干物质生产

Abstract:

【Objective】 The aim of this study was to explore the comprehensive response difference and physiological mechanism of different rice cultivars in response to elevated atmospheric CO₂ concentration and nitrogen nutrition. 【Method】 In this study, a rice cultivar Liangyoupeijiu (LY) with high response to CO₂ (high-response rice cultivar) and a rice cultivar Nanjing 9108 (NJ) with low response to CO₂(low-response rice cultivar) were selected as materials. Hydroponic experiments were carried out in the climate chamber. Two CO₂treatments and two nitrogen treatments were set up with ambient CO₂concentration (A-CO₂, 400 μmol·mol^-1) and elevated CO₂concentration (E-CO₂, 600 μmol·mol^-1), and high nitrogen (HN, 1.25 mmol·L^-1 NH₄NO₃) and low nitrogen (LN, 0.25 mmol·L^-1 NH₄NO₃), respectively. The effects of elevated CO₂ concentration on root morphology and physiological activity, cytokinin (CTKs) content in leaves and roots, nitrogen assimilation enzyme activity, physiological characteristics of leaves, photosynthetic parameters, and dry matter accumulation of different rice cultivars were analyzed. 【Result】 (1) E-CO₂ significantly increased the total crown root number, total root length (except LN level), total root surface area, and average diameter of LY, improved root respiration rate and maintained high root oxidation power, but had no significant or opposite effects on NJ. (2) Regardless of nitrogen level, E-CO₂ significantly increased CTKs content in LY leaves and roots, but significantly decreased zeatin nucleoside (ZR) content in NJ roots at HN level. (3) At LN level, E-CO₂ significantly increased GOGAT and GDH activities in LY leaves, but significantly decreased NR activities in NJ leaves. At HN level, the activity of LY nitrogen assimilation enzyme increased under E-CO₂ condition, but only NR activity increased in NJ. (4) At LN level, E-CO₂ increased the net photosynthetic rate (P_n) of LY and NJ by 28.0% and 29.4%, respectively. At HN level, P_n of the two cultivars increased by 41.0% and 28.1%, respectively. The significant increase in photosynthetic response of LY was attributed to the significant increase in leaf maximum carboxylation efficiency (V_c,max), maximum photosynthetic electron transport efficiency (J_max), ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) content, chlorophyll content, and leaf nitrogen content. (5) E-CO₂ significantly increased the leaf area per plant of LY under different nitrogen levels, but had no significant effect on NJ. (6) E-CO₂ significantly increased the organs and total biomass of LY, and the increased level under HN was significantly higher than that under LN level. E-CO₂ did not significantly affect the total biomass of NJ under different nitrogen treatments, but significantly reduced the underground biomass of NJ under HN (-16.7%). 【Conclusion】 No matter at the HN or LN treatment, the response of dry matter production and physiological characteristics of LY to E-CO₂ was higher than that of NJ. In the early growth stage, LY had better root morphological characters and root activity, higher CTKs content, stronger nitrogen assimilation ability, larger green leaf area and photosynthetic response capacity, which were important reasons accounting for the higher response of dry matter production under E-CO₂ conditions.

Key words: rice, elevated CO₂ concentration, root morphology, cytokinin, photosynthesis, dry matter production

贺江, 丁颖, 娄向弟, 姬东玲, 张向向, 王永慧, 张伟杨, 王志琴, 王伟露, 杨建昌. 水稻分蘖期干物质积累对大气CO₂浓度升高和氮素营养的综合响应差异及其生理机制[J]. 中国农业科学, 2023, 56(6): 1045-1060.

HE Jiang, DING Ying, LOU XiangDi, JI DongLing, ZHANG XiangXiang, WANG YongHui, ZHANG WeiYang, WANG ZhiQin, WANG WeiLu, YANG JianChang. Difference in the Comprehensive Response of Dry Matter Accumulation of Rice at Tillering Stage to Rising Atmospheric CO₂ Concentration and Nitrogen Nutrition and Its Physiological Mechanism[J]. Scientia Agricultura Sinica, 2023, 56(6): 1045-1060.

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N水平 N level	品种 Cultivar	二氧化碳水平CO₂level	每穴冠根数 Number of roots	每穴总根长 Total root length (cm/hill)	每穴表面积 Total root surface (cm²/hill)	平均直径 Average diameter (mm)
LN	LY	A	47.6±3.1cd	2386.1±81.6b	214.7±15.0c	0.29±0.01c
	LY	E	60.8±1.1b	2377.6±81.6b	261.1±15.3b	0.35±0.02a
	NJ	A	43.0±0.9de	2329.2±166.1b	146.3±9.1ef	0.20±0.01e
	NJ	E	43.1±2.9de	1759.5±116.7e	150.5±10.2ef	0.27±0.01cd
HN	LY	A	51.9±2.8c	2188.6±107.5bc	185.8±12.1d	0.27±0.01cd
	LY	E	77.9±4.6a	3389.2±173.1a	348.0±16.8a	0.32±0.02b
	NJ	A	40.1±2.8e	2005.9±138.3cd	166.8±8.4de	0.25±0.01d
	NJ	E	40.1±1.5e	1816.7±114.6de	142.6±8.2f	0.26±0.02d
方差分析 ANOVA
N			**	*	**	NS
Cultivar			**	**	**	**
CO₂			**	**	**	**
N×Cultivar			**	**	*	**
N×CO₂			*	**	**	**
Cultivar×CO₂			**	**	**	NS
N×Cultivar×CO₂			*	*	**	*

氮水平 N level	品种 Cultivar	二氧化碳水平 CO₂level	叶片干重 Leaf dry weight (g/hill)	茎鞘干重 Stem dry weight (g/hill)	根系干重 Root dry weight (g/hill)	地上部单茎干重 Dry weight of shoot per stem (g/stem)	根冠比 R/S
LN	LY	A	0.29±0.01de	0.23±0.01f	0.18±0.01c	0.23±0.03f	0.34±0.01b
	LY	E	0.40±0.02c	0.38±0.02b	0.28±0.01a	0.35±0.03a	0.37±0.02a
	NJ	A	0.28±0.01e	0.27±0.01de	0.11±0.01de	0.26±0.03ef	0.21±0.02c
	NJ	E	0.26±0.01e	0.26±0.01e	0.11±0.01de	0.26±0.01de	0.22±0.01c
HN	LY	A	0.49±0.03b	0.33±0.01c	0.12±0.01de	0.25±0.01ef	0.14±0.01e
	LY	E	0.96±0.03a	0.61±0.02a	0.24±0.01b	0.34±0.03ab	0.15±0.01e
	NJ	A	0.32±0.01d	0.29±0.01de	0.12±0.01d	0.30±0.01bc	0.19±0.01d
	NJ	E	0.33±0.02d	0.29±0.02d	0.10±0.01e	0.30±0.03cd	0.16±0.01e
方差分析 ANOVA
N			**	**	**	*	**
Cultivar			**	**	**	NS	**
CO₂			**	**	**	**	NS
N×Cultivar			**	**	**	NS	**
N×CO₂			**	**	NS	NS	**
Cultivar×CO₂			**	**	**	**	**
N×Cultivar×CO₂			**	**	NS	NS	NS

水稻分蘖期干物质积累对大气CO₂浓度升高和氮素营养的综合响应差异及其生理机制

Difference in the Comprehensive Response of Dry Matter Accumulation of Rice at Tillering Stage to Rising Atmospheric CO₂ Concentration and Nitrogen Nutrition and Its Physiological Mechanism

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