水稻分蘖期干物质积累对大气CO2浓度升高和氮素营养的综合响应差异及其生理机制

doi:10.3864/j.issn.0578-1752.2023.06.003

中国农业科学 ›› 2023, Vol. 56 ›› Issue (6): 1045-1060.doi: 10.3864/j.issn.0578-1752.2023.06.003

• 耕作栽培·生理生化·农业信息技术 • 上一篇下一篇

水稻分蘖期干物质积累对大气CO₂浓度升高和氮素营养的综合响应差异及其生理机制

贺江¹^,²(), 丁颖², 娄向弟², 姬东玲¹, 张向向², 王永慧², 张伟杨¹, 王志琴¹, 王伟露¹^,³(), 杨建昌¹

¹ 江苏省作物遗传生理重点实验室/江苏省作物栽培生理重点实验室/扬州大学农学院，江苏扬州 225009
² 江苏沿海地区农业科学研究所，江苏盐城 224002
³ 教育部农业与农产品安全国际合作联合实验室/扬州大学农业科技发展研究院，江苏扬州 225009

收稿日期:2022-06-28 接受日期:2022-08-02 出版日期:2023-03-16 发布日期:2023-03-23
联系方式: 贺江，E-mail：hejiang0323@163.com。
基金资助:
江苏省自然科学青年基金(BK20200923); 国家自然科学基金(32201888); 国家自然科学基金(32071943); 国家重点研发计划(SQ2022YFD2300304); 国家重点研发计划(2018YFD0300801); 江苏高校优势学科建设工程资助项目(PAPD); 扬州市“绿杨金凤”人才引进计划

Difference in the Comprehensive Response of Dry Matter Accumulation of Rice at Tillering Stage to Rising Atmospheric CO₂ Concentration and Nitrogen Nutrition and Its Physiological Mechanism

HE Jiang¹^,²(), DING Ying², LOU XiangDi², JI DongLing¹, ZHANG XiangXiang², WANG YongHui², ZHANG WeiYang¹, WANG ZhiQin¹, WANG WeiLu¹^,³(), YANG JianChang¹

¹ Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Agricultural College of Yangzhou University, Yangzhou 225009, Jiangsu
² Jiangsu Coastal Agricultural Science Research Institute, Yancheng 224002, Jiangsu
³ Joint International Research Laboratory of Agriculture and Agri-product Safety, Ministry of Education/Institutes of Agricultural Science and Technology Development of Yangzhou University, Yangzhou 225009, Jiangsu

Received:2022-06-28 Accepted:2022-08-02 Published:2023-03-16 Online:2023-03-23

摘要/Abstract

摘要：

【目的】探究不同类型水稻品种物质生产响应大气CO₂浓度升高和氮素营养的综合响应差异及其生理机制。【方法】以产量和物质生产对CO₂浓度升高响应有明显差异的水稻品种两优培九（LY）和南粳9108（NJ）为材料，在人工气候室进行水培试验。分别设置对照CO₂浓度（A-CO₂，400 μmol·mol^-1）和CO₂浓度升高（E-CO₂，600 μmol·mol^-1）两个CO₂处理，高氮（HN，1.25 mmol·L^-1 NH₄NO₃）和低氮（LN，0.25 mmol·L^-1 NH₄NO₃）两个氮水平。分析CO₂浓度升高对不同水稻品种根系形态与生理活性、叶片和根系中细胞分裂素（CTKs）含量、氮素同化酶活性、叶片生理特性、光合参数以及干物质积累的影响差异。【结果】（1）E-CO₂显著增加了LY总冠根数、总根长（LN水平除外）、总根表面积和平均直径，提高其根系呼吸速率和维持较高的根系氧化力，而对NJ无显著影响或表现相反；（2）无论氮水平如何，E-CO₂显著提高了LY叶片和根系CTKs含量，但显著降低了HN水平下NJ根系中玉米素核苷（ZR）含量；（3）在LN水平下，E-CO₂显著提高了LY叶片GOGAT、GDH活性，显著降低了NJ叶片NR活性。在HN水平下，LY氮同化酶活性在E-CO₂条件下都表现为提高，NJ仅NR活性提高；（4）在LN水平下，E-CO₂使得LY和NJ净光合速率（P_n）分别提高了28.0%和29.4%。在HN水平下，两品种分别提高了41.0%和28.1%。LY光合响应大幅度提高归因于叶片最大羧化效率（V_c,max）、最大光合电子传递效率（J _max）、核酮糖-1,5-二磷酸羧化酶（Rubisco）含量、叶绿素含量、叶片氮含量等显著提高；（5）E-CO₂显著增加了不同氮水平下LY单株叶面积，对NJ无显著影响；（6）E-CO₂显著增加了LY各器官及总生物量，且HN水平增幅明显大于LN水平。E-CO₂并未显著影响不同氮水平下NJ的总生物量，显著降低了HN水平下NJ地下部生物量（-16.7%）。【结论】无论在HN还是LN水平下，LY物质生产和生理特征对E-CO₂的响应幅度与NJ相比更高。生育前期LY较优的根系形态性状和根系活力、较高的CTKs含量、较强的氮素同化能力、较大的绿叶面积以及光合响应能力是其干物质生产对E-CO₂响应幅度较高的重要原因。

关键词: 水稻, CO₂浓度升高, 根系形态, 细胞分裂素, 光合作用, 干物质生产

Abstract:

【Objective】 The aim of this study was to explore the comprehensive response difference and physiological mechanism of different rice cultivars in response to elevated atmospheric CO₂ concentration and nitrogen nutrition. 【Method】 In this study, a rice cultivar Liangyoupeijiu (LY) with high response to CO₂ (high-response rice cultivar) and a rice cultivar Nanjing 9108 (NJ) with low response to CO₂(low-response rice cultivar) were selected as materials. Hydroponic experiments were carried out in the climate chamber. Two CO₂treatments and two nitrogen treatments were set up with ambient CO₂concentration (A-CO₂, 400 μmol·mol^-1) and elevated CO₂concentration (E-CO₂, 600 μmol·mol^-1), and high nitrogen (HN, 1.25 mmol·L^-1 NH₄NO₃) and low nitrogen (LN, 0.25 mmol·L^-1 NH₄NO₃), respectively. The effects of elevated CO₂ concentration on root morphology and physiological activity, cytokinin (CTKs) content in leaves and roots, nitrogen assimilation enzyme activity, physiological characteristics of leaves, photosynthetic parameters, and dry matter accumulation of different rice cultivars were analyzed. 【Result】 (1) E-CO₂ significantly increased the total crown root number, total root length (except LN level), total root surface area, and average diameter of LY, improved root respiration rate and maintained high root oxidation power, but had no significant or opposite effects on NJ. (2) Regardless of nitrogen level, E-CO₂ significantly increased CTKs content in LY leaves and roots, but significantly decreased zeatin nucleoside (ZR) content in NJ roots at HN level. (3) At LN level, E-CO₂ significantly increased GOGAT and GDH activities in LY leaves, but significantly decreased NR activities in NJ leaves. At HN level, the activity of LY nitrogen assimilation enzyme increased under E-CO₂ condition, but only NR activity increased in NJ. (4) At LN level, E-CO₂ increased the net photosynthetic rate (P_n) of LY and NJ by 28.0% and 29.4%, respectively. At HN level, P_n of the two cultivars increased by 41.0% and 28.1%, respectively. The significant increase in photosynthetic response of LY was attributed to the significant increase in leaf maximum carboxylation efficiency (V_c,max), maximum photosynthetic electron transport efficiency (J_max), ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) content, chlorophyll content, and leaf nitrogen content. (5) E-CO₂ significantly increased the leaf area per plant of LY under different nitrogen levels, but had no significant effect on NJ. (6) E-CO₂ significantly increased the organs and total biomass of LY, and the increased level under HN was significantly higher than that under LN level. E-CO₂ did not significantly affect the total biomass of NJ under different nitrogen treatments, but significantly reduced the underground biomass of NJ under HN (-16.7%). 【Conclusion】 No matter at the HN or LN treatment, the response of dry matter production and physiological characteristics of LY to E-CO₂ was higher than that of NJ. In the early growth stage, LY had better root morphological characters and root activity, higher CTKs content, stronger nitrogen assimilation ability, larger green leaf area and photosynthetic response capacity, which were important reasons accounting for the higher response of dry matter production under E-CO₂ conditions.

Key words: rice, elevated CO₂ concentration, root morphology, cytokinin, photosynthesis, dry matter production

贺江, 丁颖, 娄向弟, 姬东玲, 张向向, 王永慧, 张伟杨, 王志琴, 王伟露, 杨建昌. 水稻分蘖期干物质积累对大气CO₂浓度升高和氮素营养的综合响应差异及其生理机制[J]. 中国农业科学, 2023, 56(6): 1045-1060.

HE Jiang, DING Ying, LOU XiangDi, JI DongLing, ZHANG XiangXiang, WANG YongHui, ZHANG WeiYang, WANG ZhiQin, WANG WeiLu, YANG JianChang. Difference in the Comprehensive Response of Dry Matter Accumulation of Rice at Tillering Stage to Rising Atmospheric CO₂ Concentration and Nitrogen Nutrition and Its Physiological Mechanism[J]. Scientia Agricultura Sinica, 2023, 56(6): 1045-1060.

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参考文献 68

[1]	IPCC. Climate Change 2013: The Physical Science Basis// Working Group I Contribution to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, 2014.
[2]	GAMAGE D, THOMPSON M, SUTHERLAND M, HIROTSU N, MAKINO A, SENEWEERA S. New insights into the cellular mechanisms of plant growth at elevated atmospheric carbon dioxide concentrations. Plant, Cell & Environment, 2018, 41(6): 1233-1246.
[3]	WANG W L, HE J, WANG Z Q, GU J F, LIU L J, ZHANG W Y, ZISKA L H, ZHU J G. Leaf characteristics of rice cultivars with a stronger yield response to projected increases in CO₂ concentration. Physiologia Plantarum, 2021, 171(3): 416-423. doi: 10.1111/ppl.v171.3
[4]	WANG W L, CAI C, HE J, GU J F, ZHU G L, ZHANG W Y, ZHU J G, LIU G. Yield, dry matter distribution and photosynthetic characteristics of rice under elevated CO₂ and increased temperature conditions. Field Crops Research, 2020, 248: 107605. doi: 10.1016/j.fcr.2019.107605
[5]	WANG W L, CAI C, LAM S K, LIU G, ZHU J G. Elevated CO₂ cannot compensate for japonica grain yield losses under increasing air temperature because of the decrease in spikelet density. European Journal of Agronomy, 2018, 99: 21-29. doi: 10.1016/j.eja.2018.06.005
[6]	ZISKA L H, TOMECEK M B, GEALY D R. Assessment of cultivated and wild, weedy rice lines to concurrent changes in CO₂ concentration and air temperature: Determining traits for enhanced seed yield with increasing atmospheric CO₂. Functional Plant Biology, 2014, 41(3): 236. doi: 10.1071/FP13155
[7]	KIM H Y, LIEFFERING M, KOBAYASHI K, OKADA M, MITCHELL M W, GUMPERTZ M. Effects of free air CO₂ enrichment and nitrogen supply on the yield of temperate paddy rice crops. Field Crops Research, 2003, 83(3): 261-270. doi: 10.1016/S0378-4290(03)00076-5
[8]	YANG L X, WANG Y L, DONG G C, GU H, HUANG J Y, ZHU J G, YANG H J, LIU G, HAN Y. The impact of free air CO₂ enrichment (FACE) and nitrogen supply on grain quality of rice. Field Crops Research, 2007, 102(2): 128-140. doi: 10.1016/j.fcr.2007.03.006
[9]	CHEN C, JIANG Q, ZISKA L H, ZHU J G, LIU G, ZHANG J S, NI K, SENEWEERA S, ZHU C W. Seed vigor of contrasting rice cultivars in response to elevated carbon dioxide. Field Crops Research, 2015, 178: 63-68. doi: 10.1016/j.fcr.2015.03.023
[10]	YANG L X, LIU H J, WANG Y X, ZHU J G, HUANG J Y, LIU G, DONG G C, WANG Y L. Yield formation of CO₂enriched inter-subspecific hybrid rice cultivar Liangyoupeijiu under fully open-air field condition in a warm sub-tropical climate. Agriculture, Ecosystems & Environment, 2009, 129(1/3): 193-200. doi: 10.1016/j.agee.2008.08.016
[11]	ZHU C W, ZHU J G, CAO J, JIANG Q, LIU G, ZISKA L H. Biochemical and molecular characteristics of leaf photosynthesis and relative seed yield of two contrasting rice cultivars in response to elevated CO₂. Journal of Experimental Botany, 2014, 65(20): 6049-6056. doi: 10.1093/jxb/eru344
[12]	YANG L X, LIU H J, WANG Y X, ZHU J G, HUANG J Y, GANG L, DONG G C, WANG Y L. Impact of elevated CO₂ concentration on inter-subspecific hybrid rice cultivar Liangyoupeijiu under fully open-air field conditions. Field Crops Research, 2009, 112(1): 7-15. doi: 10.1016/j.fcr.2009.01.008
[13]	周娟, 舒小伟, 赖上坤, 许高平, 黄建晔, 姚友礼, 杨连新, 董桂春, 王余龙. 不同类型水稻品种产量和氮素吸收利用对大气CO₂浓度升高响应的差异. 中国水稻科学, 2020, 34(6): 561-573. doi: 10.16819/j.1001-7216.2020.0404
	ZHOU J, SHU X W, LAI S K, XU G P, HUANG J Y, YAO Y L, YANG L X, DONG G C, WANG Y L. Differences in response of grain yield, nitrogen absorption and utilization to elevated CO₂ concentration in different rice varieties. Chinese Journal of Rice Science, 2020, 34(6): 561-573. (in Chinese)
[14]	CHEN C P, SAKAI H, TOKIDA T, USUI Y, NAKAMURA H, HASEGAWA T. Do the rich always become richer? Characterizing the leaf physiological response of the high-yielding rice cultivar takanari to free air CO₂ enrichment. Plant & Cell Physiology, 2014, 55(2): 381-391.
[15]	DAEPP M, SUTER D, ALMEIDA J P F, ISOPP H, HARTWIG U A, FREHNER M, BLUM H, NÖSBERGER J, LÜSCHER A. Yield response of Lolium perenne swards to free air CO₂ enrichment increased over six years in a high N input system on fertile soil. Global Change Biology, 2000, 6(7): 805-816. doi: 10.1046/j.1365-2486.2000.00359.x
[16]	STITT M, KRAPP A. The interaction between elevated carbon dioxide and nitrogen nutrition: The physiological and molecular background. Plant, Cell and Environment, 1999, 22: 583-621. doi: 10.1046/j.1365-3040.1999.00386.x
[17]	TERASHIMA I, YANAGISAWA S, SAKAKIBARA H. Plant responses to CO₂: Background and perspectives. Plant & Cell Physiology, 2014, 55(2): 237-240.
[18]	FENG Z Z, RÜTTING T, PLEIJEL H, WALLIN G, REICH P B, KAMMANN C I, NEWTON P C D, KOBAYASHI K, LUO Y J, UDDLING J. Constraints to nitrogen acquisition of terrestrial plants under elevated CO₂. Global Change Biology, 2015, 21(8): 3152-3168. doi: 10.1111/gcb.12938 pmid: 25846203
[19]	戢林, 李廷轩, 张锡洲, 余海英. 氮高效利用基因型水稻根系形态和活力特征. 中国农业科学, 2012, 45(23): 4770-4781. doi: 10.3864/j.issn.0578-1752.2012.23.003
	JI L, LI T X, ZHANG X Z, YU H Y. Root morphological and activity characteristics of rice genotype with high nitrogen utilization efficiency. Scientia Agricultura Sinica, 2012, 45(23): 4770-4781. (in Chinese) doi: 10.3864/j.issn.0578-1752.2012.23.003
[20]	杨建昌. 水稻根系形态生理与产量、品质形成及养分吸收利用的关系. 中国农业科学, 2011, 44(1): 36-46.
	YANG J C. Relationships of rice root morphology and physiology with the formation of grain yield and quality and the nutrient absorption and utilization. Scientia Agricultura Sinica, 2011, 44(1): 36-46. (in Chinese)
[21]	YANG L X, WANG Y L, KOBAYASHI K, ZHU J G, HUANG J Y, YANG H J, WANG Y X, DONG G C, LIU G, HAN Y, SHAN Y H, HU J, ZHOU J. Seasonal changes in the effects of free-air CO₂ enrichment (FACE) on growth, morphology and physiology of rice root at three levels of nitrogen fertilization. Global Change Biology, 2008, 14(8): 1844-1853. doi: 10.1111/j.1365-2486.2008.01624.x
[22]	武慧斌, 宋正国, 沈跃, 唐世荣, 刘仲齐. 水稻根系生长发育对CO₂浓度升高的响应及其品种间的差异. 生态环境学报, 2014, 23(3): 439-443.
	WU H B, SONG Z G, SHEN Y, TANG S R, LIU Z Q. Response of root development on elevated CO₂ and its variation among different rice varieties. Ecology and Environmental Sciences, 2014, 23(3): 439-443. (in Chinese)
[23]	庞静, 朱建国, 谢祖彬, 刘钢, 陈改苹, 张雅丽. 开放式空气二氧化碳浓度增高(FACE)条件下水稻的根系活力和氮同化能力. 应用生态学报, 2005, 16(8): 1482-1486.
	PANG J, ZHU J G, XIE Z B, LIU G, CHEN G P, ZHANG Y L. Root activity and nitrogen assimilation of rice (Oryza sativa L.) under free-air CO₂ enrichment. Chinese Journal of Applied Ecology, 2005, 16(8): 1482-1486. (in Chinese)
[24]	OOKAWA T, NARUOKA Y, SAYAMA A, HIRASAWA T. Cytokinin effects on ribulose-1,5-bisphosphate carboxylase/oxygenase and nitrogen partitioning in rice during ripening. Crop Science, 2004, 44(6): 2107-2115. doi: 10.2135/cropsci2004.2107
[25]	REGUERA M, PELEG Z, ABDEL-TAWAB Y M, TUMIMBANG E B, DELATORRE C A, BLUMWALD E. Stress-induced cytokinin synthesis increases drought tolerance through the coordinated regulation of carbon and nitrogen assimilation in rice. Plant Physiology, 2013, 163(4): 1609-1622. doi: 10.1104/pp.113.227702 pmid: 24101772
[26]	CRIADO M V, CAPUTO C, ROBERTS I N, CASTRO M A, BARNEIX A J. Cytokinin-induced changes of nitrogen remobilization and chloroplast ultrastructure in wheat (Triticum aestivum). Journal of Plant Physiology, 2009, 166(16): 1775-1785. doi: 10.1016/j.jplph.2009.05.007 pmid: 19540618
[27]	HE J, ZHANG W Y, ZHU K Y, WANG Z Q, WANG W L, YANG J C. Advantages linked to root development enhance rice biomass accumulation under elevated carbon dioxide conditions. Agronomy Journal, 2020, 112(5): 4007-4017. doi: 10.1002/agj2.v112.5
[28]	SHARKEY T D, BERNACCHI C J, FARQUHAR G D, SINGSAAS E L. Fitting photosynthetic carbon dioxide response curves for C₃ leaves. Plant, Cell & Environment, 2007, 30(9): 1035-1040.
[29]	LICHTENTHALER H K, WELLBURN A R. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions, 1983, 11(5): 591-592. doi: 10.1042/bst0110591
[30]	MAKINO A, OHIRA T M. Photosynthesis and ribulose-1,5- bisphosphate carboxylase/oxygenase in rice leaves from emergence through senescence. Planta, 1985, 166(3): 414-420. doi: 10.1007/BF00401181
[31]	MAKINO A, MAE T, OHIRA K. Colorimetric measurement of protein stained with coomassie brilliant blue R on sodium dodecyl sulfate-polyacrylamide gel electrophoresis by eluting with formamide. Agricultural and Biological Chemistry, 1986, 50(7): 1911-1912.
[32]	陈薇, 张德颐. 植物组织中硝酸还原酶的提取、测定和纯化. 植物生理学通讯, 1980(4): 45-49.
	CHEN W, ZHANG D Y. Extraction, determination and purification of nitrate reductase from Plant tissues. Plant Physiology Journal, 1980(4): 45-49. (in Chinese)
[33]	RHODES D, RENDON G A, STEWART G R. The control of glutamine synthetase level in Lemna minor L. Planta, 1975, 125(3): 201-211. doi: 10.1007/BF00385596
[34]	LIN C C, KAO C H. Disturbed ammonium assimilation is associated with growth inhibition of roots in rice seedlings caused by NaCl. Plant Growth Regulation, 1996, 18(3): 233-238. doi: 10.1007/BF00024387
[35]	ANDO T, YOSHIDA S, NISHIYAMA I. Nature of oxidizing power of rice roots. Plant & Soil, 1983, 72(1): 57-71.
[36]	ZHANG H, CHEN T T, WANG Z Q, YANG J C, ZHANG J H. Involvement of cytokinins in the grain filling of rice under alternate wetting and drying irrigation. Journal of Experimental Botany, 2010, 61(13): 3719-3733. doi: 10.1093/jxb/erq198 pmid: 20584789
[37]	YANG J C, ZHANG H, ZHANG J H. Root morphology and physiology in relation to the yield formation of rice. Journal of Integrative Agriculture, 2012, 11(6): 920-926. doi: 10.1016/S2095-3119(12)60082-3
[38]	李志康, 严冬, 薛张逸, 顾逸彪, 李思嘉, 刘立军, 张耗, 王志琴, 杨建昌, 顾骏飞. 细胞分裂素对植物生长发育的调控机理研究进展及其在水稻生产中的应用探讨. 中国水稻科学, 2018, 32(4): 311-324. doi: 10.16819/j.1001-7216.2018.8027
	LI Z K, YAN D, XUE Z Y, GU Y B, LI S J, LIU L J, ZHANG H, WANG Z Q, YANG J C, GU J F. Regulations of plant growth and development by cytokinins and their applications in rice production. Chinese Journal of Rice Science, 2018, 32(4): 311-324. (in Chinese) doi: 10.16819/j.1001-7216.2018.8027
[39]	KIBA T, KUDO T, KOJIMA M, SAKAKIBARA H. Hormonal control of nitrogen acquisition: Roles of auxin, abscisic acid, and cytokinin. Journal of Experimental Botany, 2011, 62(4): 1399-1409. doi: 10.1093/jxb/erq410 pmid: 21196475
[40]	OOKAWA T, NARUOKA Y, YAMAZAKI T, SUGA J, HIRASAWA T. A comparison of the accumulation and partitioning of nitrogen in plants between two rice cultivars, akenohoshi and nipponbare, at the ripening stage. Plant Production Science, 2003, 6(3): 172-178. doi: 10.1626/pps.6.172
[41]	SCHAZ U, DÜLL B, REINBOTHE C, BECK E. Influence of root-bed size on the response of tobacco to elevated CO₂ as mediated by cytokinins. AoB Plants, 2014, 10(6): 490-552.
[42]	WANG Y, DU S T, LI L L, HUANG L D, FANG P, LIN X Y, ZHANG Y S, WANG H L. Effect of CO₂ elevation on root growth and its relationship with indole acetic acid and ethylene in tomato seedlings. Pedosphere, 2009, 19(5): 570-576. doi: 10.1016/S1002-0160(09)60151-X
[43]	LI X M, ZHANG L H, MA L J, LI Y Y. Elevated carbon dioxide and/or ozone concentrations induce hormonal changes in Pinus tabulaeformis. Journal of Chemical Ecology, 2011, 37(7): 779-784. doi: 10.1007/s10886-011-9975-7
[44]	LI C R, GAN L J, XIA K, ZHOU X, HEW C S. Responses of carboxylating enzymes, sucrose metabolizing enzymes and plant hormones in a tropical epiphytic CAM orchid to CO₂ enrichment. Plant, Cell & Environment, 2010, 25(3): 369-377.
[45]	YONG J W H, WONG S C, LETHAM D S, HOCART C H, FARQUHAR G D. Effects of elevated CO₂ and nitrogen nutrition on cytokinins in the xylem sap and leaves of cotton. Plant Physiology, 2000, 124(2): 767-780. doi: 10.1104/pp.124.2.767
[46]	WANG W L, XU X, ZHU C W, GU J F, ZHANG W Y, LIU G, ZHU J G. Elevated CO₂induced changes in cytokinin and nitrogen metabolism are associated with different responses in the panicle architecture of two contrasting rice genotypes. Plant Growth Regulation, 2019, 89(2): 119-129. doi: 10.1007/s10725-019-00511-4
[47]	XU G H, FAN X R, MILLER A J. Plant nitrogen assimilation and use efficiency. Annual Review of Plant Biology, 2012, 63(1): 153-182. doi: 10.1146/arplant.2012.63.issue-1
[48]	ROBREDO A, PÉREZ-LÓPEZ U, MIRANDA-APODACA J, LACUESTA M, MENA-PETITE A, MUÑOZ-RUEDA A. Elevated CO₂ reduces the drought effect on nitrogen metabolism in barley plants during drought and subsequent recovery. Environmental and Experimental Botany, 2011, 71(3): 399-408.
[49]	门中华, 李生秀. CO₂浓度对冬小麦氮代谢的影响. 中国农业科学, 2005, 38(2): 320-326.
	MEN Z H, LI S X. Effect of CO₂ concentration on nitrogen metabolism of winter wheat. Scientia Agricultura Sinica, 2005, 38(2): 320-326. (in Chinese)
[50]	BLOOM A J, BURGER M, ASENSIO J S R, COUSINS A B. Carbon dioxide enrichment inhibits nitrate assimilation in wheat and Arabidopsis. Science, 2010, 328(5980): 899-903. doi: 10.1126/science.1186440
[51]	赵黎明, 李明, 郑殿峰, 顾春梅, 那永光, 解保胜. 灌溉方式与种植密度对寒地水稻产量及光合物质生产特性的影响. 农业工程学报, 2015, 31(6): 159-169.
	ZHAO L M, LI M, ZHENG D F, GU C M, NA Y G, XIE B S. Effects of irrigation methods and rice planting densities on yield and photosynthetic characteristics of matter production in cold area. Transactions of the Chinese Society of Agricultural Engineering, 2015, 31(6): 159-169. (in Chinese)
[52]	WANG J Y, WANG C, CHEN N N, XIONG Z Q, WOLFE D, ZOU J W. Response of rice production to elevated CO₂ and its interaction with rising temperature or nitrogen supply: a meta-analysis. Climatic Change, 2015, 130(4): 529-543. doi: 10.1007/s10584-015-1374-6
[53]	杨海龙, 蔡金洋. 大气CO₂浓度和温度升高对水稻生长发育影响的研究进展. 安徽农业科学, 2020, 48(4): 24-27, 30.
	YANG H L, CAI J Y. A review on the effects of increasing atmospheric CO₂ concentration and temperature on rice growth and development. Journal of Anhui Agricultural Sciences, 2020, 48(4): 24-27, 30. (in Chinese)
[54]	李彦生, 金剑, 刘晓冰. 作物对大气CO₂浓度升高生理响应研究进展. 作物学报, 2020, 46(12): 1819-1830. doi: 10.3724/SP.J.1006.2020.02027
	LI Y S, JIN J, LIU X B. Physiological response of crop to elevated atmospheric carbon dioxide concentration: a review. Acta Agronomica Sinica, 2020, 46(12): 1819-1830. (in Chinese)
[55]	谢立勇, 姜乐, 冯永祥, 赵洪亮, 王惠贞, 林而达. FACE条件下CO₂浓度和温度增高对北方水稻光合作用与产量的影响研究. 中国农业大学学报, 2014, 19(3): 101-107.
	XIE L Y, JIANG L, FENG Y X, ZHAO H L, WANG H Z, LIN E D. Impacts of elevated CO₂and increasing temperature on rice photosynthesis and yield in North China under FACE system. Journal of China Agricultural University, 2014, 19(3): 101-107. (in Chinese)
[56]	CAI C, YIN X Y, HE S Q, JIANG W Y, SI C F, STRUIK P C, LUO W H, LI G, XIE Y T, XIONG Y, PAN G X. Responses of wheat and rice to factorial combinations of ambient and elevated CO₂ and temperature in FACE experiments. Global Change Biology, 2016, 22(2): 856-874. doi: 10.1111/gcb.13065
[57]	ARANJUELO I, ERICE G, SANZ-SAEZ A, ABADIE C, GILARD F, GIL-QUINTANA E, AVICE J C, STAUDINGER C, WIENKOOP S, ARAUS J L, BOURGUIGNON J, IRIGOYEN J J, TCHERKEZ G. Differential CO₂ effect on primary carbon metabolism of flag leaves in durum wheat (Triticum durum Desf.). Plant, Cell & Environment, 2015, 38(12): 2780-2794.
[58]	SENEWEERA S, MAKINO A, HIROTSU N, NORTON R, SUZUKI Y. New insight into photosynthetic acclimation to elevated CO₂: The role of leaf nitrogen and ribulose-1,5-bisphosphate carboxylase/ oxygenase content in rice leaves. Environmental and Experimental Botany, 2011, 71(2): 128-136. doi: 10.1016/j.envexpbot.2010.11.002
[59]	LI Y, GAO Y X, XU X M, SHEN Q R, GUO S W. Light-saturated photosynthetic rate in high-nitrogen rice (Oryza sativa L.) leaves is related to chloroplastic CO₂ concentration. Journal of Experimental Botany, 2009, 60(8): 2351-2360. doi: 10.1093/jxb/erp127
[60]	邹应斌, 黄见良, 屠乃美, 李合松, 黄升平, 张杨珠. "旺壮重"栽培对双季杂交稻产量形成及生理特性的影响. 作物学报, 2001, 27(3): 343-350.
	ZOU Y B, HUANG J L, TU N M, LI H S, HUANG S P, ZHANG Y Z. Effects of the VSW cultural method on yield formation and physiological characteristics in double cropping hybrid rice. Acta Agronomica Sinica, 2001, 27(3): 343-350. (in Chinese)
[61]	AINSWORTH E A, LONG S P. What have we learned from 15 years of free-air CO₂ enrichment (FACE)? A meta-analytic review of the responses of photosynthesis, canopy properties and plant production to rising CO₂. New Phytologist, 2004, 165(2): 351-372. doi: 10.1111/nph.2005.165.issue-2
[62]	KIM H, SUNG N. The effect of ambient pressure on the evaporation of a single droplet and a spray. Combustion and Flame, 2003, 135(3): 261-270. doi: 10.1016/S0010-2180(03)00165-2
[63]	刘红江, 杨连新, 黄建晔, 董桂春, 朱建国, 刘钢, 王余龙. FACE对杂交籼稻汕优63干物质生产与分配的影响. 农业环境科学学报, 2009, 28(1): 8-14.
	LIU H J, YANG L X, HUANG J Y, DONG G C, ZHU J G, LIU G, WANG Y L. Effect of free air CO₂ enrichment (FACE) on production and distribution of dry matter of three-line indica hybrid rice cultivar Shanyou 63. Journal of Agro-Environment Sciences, 2009, 28(1): 8-14. (in Chinese)
[64]	YANG L X, HUANG J Y, YANG H J, DONG G C, LIU G, ZHU J G, WANG Y L. Seasonal changes in the effects of free-air CO₂ enrichment (FACE) on dry matter production and distribution of rice (Oryza sativa L.). Field Crops Research, 2006, 98(1): 12-19. doi: 10.1016/j.fcr.2005.11.003
[65]	李春华, 曾青, 沙霖楠, 张继双, 朱建国, 刘钢. 大气CO₂浓度和温度升高对水稻地上部干物质积累和分配的影响. 生态环境学报, 2016, 25(8): 1336-1342. doi: 10.16258/j.cnki.1674-5906.2016.08.012
	LI C H, ZENG Q, SHA L N, ZHANG J S, ZHU J G, LIU G. Impacts of elevated atmospheric CO₂ and temperature on above-ground dry matter accumulation and distribution of rice (Oryza sativa L.). Ecology and Environmental Sciences, 2016, 25(8): 1336-1342. (in Chinese)
[66]	陈改苹, 朱建国, 谢祖彬, 朱春梧, 程磊, 曾青, 庞静. 开放式空气CO₂浓度升高对水稻根系形态的影响. 生态环境, 2005, 14(4): 503-507.
	CHEN G P, ZHU J G, XIE Z B, ZHU C W, CHENG L, ZENG Q, PANG J. Effects of free air CO₂ enrichment on root morphology of rice. Ecology and Environment, 2005, 14(4): 503-507. (in Chinese)
[67]	张凤哲, 谢立勇, 赵洪亮, 金殿玉. 大气CO₂浓度升高条件下施加生物炭对水稻生物量分配及产量的影响. 植物营养与肥料学报, 2021, 27(6): 929-937.
	ZHANG F Z, XIE L Y, ZHAO H L, JIN D Y. Synergistic effects of biochar application and elevated atmospheric CO₂ concentration on rice biomass allocation and yield. Plant Nutrition and Fertilizer Science, 2021, 27(6): 929-937. (in Chinese)
[68]	WU J J, KRONZUCKER H J, SHI W M. Dynamic analysis of the impact of free-air CO₂ enrichment (FACE) on biomass and N uptake in two contrasting genotypes of rice. Functional Plant Biology, 2018, 45(7): 696-704. doi: 10.1071/FP17278

N水平 N level	品种 Cultivar	二氧化碳水平CO₂level	每穴冠根数 Number of roots	每穴总根长 Total root length (cm/hill)	每穴表面积 Total root surface (cm²/hill)	平均直径 Average diameter (mm)
LN	LY	A	47.6±3.1cd	2386.1±81.6b	214.7±15.0c	0.29±0.01c
	LY	E	60.8±1.1b	2377.6±81.6b	261.1±15.3b	0.35±0.02a
	NJ	A	43.0±0.9de	2329.2±166.1b	146.3±9.1ef	0.20±0.01e
	NJ	E	43.1±2.9de	1759.5±116.7e	150.5±10.2ef	0.27±0.01cd
HN	LY	A	51.9±2.8c	2188.6±107.5bc	185.8±12.1d	0.27±0.01cd
	LY	E	77.9±4.6a	3389.2±173.1a	348.0±16.8a	0.32±0.02b
	NJ	A	40.1±2.8e	2005.9±138.3cd	166.8±8.4de	0.25±0.01d
	NJ	E	40.1±1.5e	1816.7±114.6de	142.6±8.2f	0.26±0.02d
方差分析 ANOVA
N			**	*	**	NS
Cultivar			**	**	**	**
CO₂			**	**	**	**
N×Cultivar			**	**	*	**
N×CO₂			*	**	**	**
Cultivar×CO₂			**	**	**	NS
N×Cultivar×CO₂			*	*	**	*

氮水平 N level	品种 Cultivar	二氧化碳水平 CO₂level	叶片干重 Leaf dry weight (g/hill)	茎鞘干重 Stem dry weight (g/hill)	根系干重 Root dry weight (g/hill)	地上部单茎干重 Dry weight of shoot per stem (g/stem)	根冠比 R/S
LN	LY	A	0.29±0.01de	0.23±0.01f	0.18±0.01c	0.23±0.03f	0.34±0.01b
	LY	E	0.40±0.02c	0.38±0.02b	0.28±0.01a	0.35±0.03a	0.37±0.02a
	NJ	A	0.28±0.01e	0.27±0.01de	0.11±0.01de	0.26±0.03ef	0.21±0.02c
	NJ	E	0.26±0.01e	0.26±0.01e	0.11±0.01de	0.26±0.01de	0.22±0.01c
HN	LY	A	0.49±0.03b	0.33±0.01c	0.12±0.01de	0.25±0.01ef	0.14±0.01e
	LY	E	0.96±0.03a	0.61±0.02a	0.24±0.01b	0.34±0.03ab	0.15±0.01e
	NJ	A	0.32±0.01d	0.29±0.01de	0.12±0.01d	0.30±0.01bc	0.19±0.01d
	NJ	E	0.33±0.02d	0.29±0.02d	0.10±0.01e	0.30±0.03cd	0.16±0.01e
方差分析 ANOVA
N			**	**	**	*	**
Cultivar			**	**	**	NS	**
CO₂			**	**	**	**	NS
N×Cultivar			**	**	**	NS	**
N×CO₂			**	**	NS	NS	**
Cultivar×CO₂			**	**	**	**	**
N×Cultivar×CO₂			**	**	NS	NS	NS

水稻分蘖期干物质积累对大气CO₂浓度升高和氮素营养的综合响应差异及其生理机制

Difference in the Comprehensive Response of Dry Matter Accumulation of Rice at Tillering Stage to Rising Atmospheric CO₂ Concentration and Nitrogen Nutrition and Its Physiological Mechanism

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