不同气候与施肥条件下农田土壤微生物生物量特征与容量分析

doi:10.3864/j.issn.0578-1752.2017.06.008

摘要/Abstract

摘要： 【目的】土壤微生物生物量是土壤生物肥力的重要指标，是土壤养分重要的周转库。探讨不同气候和施肥条件下土壤微生物生物量（生物量碳、氮）的特征及容量，对于深刻认识土壤微生物生物量的影响因素及提高土壤生物肥力具有重要意义。【方法】本研究从中国知网、万方和web of Science 3个文献数据库，以“土壤微生物生物量”、“中国农田”和“长期施肥”为关键词，共收集目标文献42篇，包括458组含土壤有机碳（SOC）与土壤微生物生物量碳（SMBC）和414组含土壤全氮（TN）与土壤微生物生物量氮（SMBN）的数据集，涵盖了4种气候下的2类施肥条件（施有机肥：单施或配施，＋OM；不施有机肥：无肥和化肥，－OM）。土壤微生物熵（SMBC/SOC）和SMBN/TN的中值差异性均采用Kruskal-Wallis H 单向显著性检验（P＜0.05），容量分析采用界限分析方法。【结果】统计分析结果表明，不同施肥处理下，SMBC与SOC和SMBN与TN之间均存在显著线性正相关关系（P＜0.01），长期施用有机肥条件下，土壤微生物生物量碳、氮对土壤有机碳和全氮增加的响应系数分别为24.77和30.27，显著高于化肥或不施肥条件（分别为19.88和19.86）（P＜0.05）。界限分析结果显示，不同施肥措施下SMBC对SOC增加响应的最大值为33.45—36.00，SMBN对TN的最大响应系数为45.45—49.79，当前条件下SMBC和SMBN还有37.99%和49.66%的提升空间。不同气候条件下SMBC/SOC和SMBN/TN均存在显著差异（P＜0.05），其中，中温带半干旱半湿润区SMBC/SOC的中值最高为2.73%，其次为亚热带湿润区（2.45%）和暖温带湿润区（2.31%），中温带湿润区最低为1.48%；SMBN/TN的中值大小顺序为：暖温带湿润区（4.72%）＞中温带半干旱半湿润区（3.50%）＞亚热带湿润区（2.99%）＞中温带湿润区（1.80%）。不同施肥条件下SMBC/SOC和SMBN/TN的变化范围分别为0.35%—6.50%和0.50%—9.72%，但其中值并无显著差异（P＞0.05）。对于同一气候条件不同施肥措施而言，仅在中温带湿润区，施有机肥处理对微生物量碳（氮）占总有机碳（氮）的比例有显著影响（P＜0.05）。【结论】气候对土壤微生物生物量碳、氮所占比例具有显著影响，不同施肥模式虽然不能显著改变微生物生物量碳、氮的比例，但有机肥的施用对微生物生物量碳、氮的提升效果显著高于化肥或不施肥，该结果对于土壤生物肥力的调控有重要指导意义。

关键词: 中国农田, 施肥, 气候, 土壤微生物熵（SMBC/SOC）, 土壤微生物生物量碳, 土壤微生物生物量氮

Abstract: 【Objective】Soil microbial biomass is an important index of soil biological fertility and also is an important pool of soil nutrient turnover. The objective of this study was to explore the capacity and characteristics of soil microbial biomass carbon (SMBC) and nitrogen (SMBN) under various climatic zones and fertilization managements across China cropland, and further to obtain better understanding of the factors and how to manage soil biological fertility.【Method】The publications were collected by searching Web of China Knowledge Resource Integrated Database, Wan Fang Database and Web of Science with the keywords of "soil microbial biomass", "Chinese cropland" and "long-term fertilization". A data set with 458 pairs of reported soil organic carbon (SOC) and SMBC and 414 pairs of reported total nitrogen (TN) and SMBN was set up from 42 published papers. They included such four categories of climatic zone as typical temperate (M1: humid; M2: semi-arid), warm-temperate (W) and subtropical (S) zones and two categories of fertilization with (+OM) and without (-OM) organic amendments across China. Statistical analysis on the median of SMBC/SOC and SMBN/TN under different fertilization managements and climate zones was performed with Kruskal-Wallis H test (P<0.05). The capacity of SMBC and SMBN to SOC and TN was obtained from boundary line, respectively.【Result】Results showed that there was a significant response of soil microbial biomass (SMBC and SMBN) to the increase in SOC and TN under different fertilization managements and climate zones (P<0.01), respectively. The response coefficients (i.e., the correlation slope) between SMBC and SOC, SMBN and TN were 24.77 and 30.27 under organic amendments, respectively, which were significantly higher than that under the management without organic amendments (19.88 for SMBC and 19.86 for SMBN) (P<0.05). Boundary analysis showed that the maximum response coefficients of SMBC to SOC and SMBN to TN were 33.45-36.00 and 45.45-49.79, respectively. Compared with the maximum values, the current average values still have 37.99% and 49.66% of room for improvement, respectively. The median values of SMBC/SOC and SMBN/TN were significantly different among the four climate zones. The highest value of SMBC/SOC was 2.73% under temperate semi-arid and sub-humid zone (M2), followed by sub-tropical (S, 2.45%) and warm humid zone (W, 2.31%), the lowest value is 1.48% under temperate humid zone (M1). The sequence of SMBN/TN was W (4.72%) >M2 (3.50%) >S (2.99%) >M1 (1.80%). The ranges of SMBC/SOC and SMBN/TN were 0.35%-6.50% and 0.50%-9.72%, respectively, under various fertilization conditions with no significant differences. For different fertilization managements under similar climatic zone, the median value of SMBC/SOC and SMBN/TN was significantly higher under organic fertilizer management than that under no organic fertilizer management under temperate humid zone (P<0.05).【Conclusion】Climatic conditions have a significant impact on soil microbial biomass capacity. Different fertilization managements do not significantly change the SMBC/SOC and SMBN/TN, but the organic amendment can significantly improve SMBC and SMBN, which are helpful for soil biological fertility improvement.

Key words: China cropland, fertilization management, climate zone, SMBC/SOC, soil microbial biomass carbon, soil microbial biomass nitrogen

王传杰，肖婧，蔡岸冬，张文菊，徐明岗. 不同气候与施肥条件下农田土壤微生物生物量特征与容量分析[J]. 中国农业科学, 2017, 50(6): 1067-1075.

WANG ChuanJie, XIAO Jing, CAI AnDong, ZHANG WenJu, XU MingGang. Capacity and Characteristics of Soil Microbial Biomass Under Various Climate and Fertilization Conditions Across China Croplands[J]. Scientia Agricultura Sinica, 2017, 50(6): 1067-1075.

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参考文献

[1] 宋长青, 吴金水, 陆雅海, 沈其荣, 贺纪正, 黄巧云, 贾仲君, 冷疏影, 朱永官. 中国土壤微生物学研10年回顾. 地球科学进展, 2013, 28(10): 1087-1105.

Song C Q, Wu J S, Lu Y H, Shen Q R, He J Z, Huang Q Y, Jia Z J, Leng S Y, Zhu Y G. Advances of soil microbiology in the last decade in China. Advances in Earth Science, 2013, 28(10): 1087-1105. (in Chinese)

[2] Ocio J A, Martimez J, Brookes P C. Contribution of straw-derived N to total microbial biomass N following incorporation of cereal straw to soil. Soil Biology and Biochemistry, 1991, 23(7): 655-659.

[3] Brookes P C, Powlson D S, Jenkinson D S. Phosphorus in the soil microbial biomass. Soil Biology and Biochemistry, 1984, 16(2): 169-175.

[4] 徐阳春, 沈其荣, 冉炜. 长期免耕与施用有机肥对土壤微生物生物量碳、氮、磷的影响. 土壤学报, 2002, 39(1): 89-96.

Xu Y C, Shen Q Y, Ran W. Effects of zero-tillage and application of manure on soil microbial biomass C, N and P after sixteen years of cropping. Acta Pedologica Sinica, 2002, 39(1): 89-96. (in Chinese)

[5] 张金波, 宋长春. 土地利用方式对土壤碳库影响的敏感性评价指标. 生态环境, 2003, 12(4): 500-504.

Zhang J B, Song C C. The sensitive evaluation indicators of effects of land-use change on soil carbon pool. Ecology and Environment, 2003, 12(4): 500-504. (in Chinese)

[6] Kennday A C, Papendick R I. Microbial characteristics of soil quality. Journal of Soil and Water Conservation, 1995, 50(3): 243-248.

[7] Bunemanna E K, Bossiob D A, Smithsonb P C, Frossard E, Oberson A. Microbial community composition and substrate use in a highly weathered soil as affected by crop rotation and P fertilization. Soil Biology and Biochemistry, 2004, 36(6): 889-901.

[8] Anderson T H, Domsch K H. Ratios of microbial biomass to total organic carbon in arable soils. Soil Biology and Biochemistry, 1989, 21(4): 471-479.

[9] Insam H, Parkinson D, Domsch K H. Influence of macroclimate of soil microbial biomass. Soil Biology and Biochemistry, 1989, 21(2): 211-221.

[10] Contin M, Corcimaru S, Nobili MD, Brookers P C. Temperature changes and the ATP concentrations of the soil microbial biomass. Soil Biology and Biochemistry,2000, 32(8/9): 1219-1225.

[11] Kandeler E, Tscherko D, Bardgett R D, Hobbs P J, Kampichier C. The response of soil micro-organisms and roots to elevated CO₂ and temperature in a terrestrial model ecosystem. Plant and Soil, 1998, 202(2): 251-262.

[12] Verburg P S J, VanDam D, Hefting M, Tietema A. Microbial transformations of C and N in a boreal forest floor as affected by temperature. Plant and Soil, 1999, 208(2): 187-197.

[13] Domisch T, Finer L, Lehto T, Smolander A. Effect of soil temperature on nutrient allocation and mycorrhizas in Scots pine seedlings. Plant and Soil, 2002, 239(2): 173-185.

[14] Joergensen R G, Brookes P C, Jenkinson D S. Survival of the microbial biomass at elevated temperatures. Soil Biology and Biochemistry, 1990, 22(8): 1129-1136.

[15] Bottner P. Response of microbial biomass to alternate moist and dry conditions in a soil incubated with ¹⁴C and ¹⁵N-labelled plant material. Soil Biology and Biochemistry, 1985, 17(3): 329-337.

[16] Rosacker L L, Kieft T L. Biomass and adenylate energy charge of a grassland soil during drying. Soil Biology and Biochemistry, 1990, 22(8): 1121-1127 .

[17] Kieft T L, Sorocer E, Firestone M K. Microbial biomass response to a rapid increase in water potential when dry soil is wetted. Soil Biology and Biochemistry, 1987, 19(2): 119-126.

[18] Plaza C, Hernández D, García-Gil J C, Polo A. Microbial activity in pig slurry-amended soils under semiarid conditions. Soil Biology and Biochemistry, 2004, 36(10): 1577-1585.

[19] KAUTZ T, WIRTH S, ELLMER F. Microbial activity in a sandy arable soil is governed by the fertilization regime. European Journal of Soil Biology, 2004, 40(2): 87-94.

[20] 臧逸飞, 郝明德, 张丽琼, 张昊青. 26年长期施肥对土壤微生物量碳、氮及土壤呼吸的影响. 生态学报, 2015, 35(5): 1445-1451.

Zang Y F, Hao M D, Zhang L Q, Zhang H Q. Effects of wheat cultivation and fertilization on soil microbial biomass carbon, soil microbial biomass nitrogen and soil basal respiration in 26 years. Acta Ecologica Sinica, 2015, 35(5): 1445-1451. (in Chinese)

[21] 马晓霞, 王莲莲, 黎青慧, 李花, 张树兰, 孙本华, 杨学云. 长期施肥对玉米生育期土壤微生物量碳氮及酶活性的影响. 生态学报, 2012, 32(17): 5502-5511.

Ma X X, Wang L L, Li Q H, Li H, Zhang S L, Sun B H, Yang X Y. Effects of long-term fertilization on soil microbial biomass carbon and nitrogen and enzyme activities during maize growing season. Acta Ecologica Sinica, 2012, 32(17): 5502-5511. (in Chinese)

[22] Witter E, Martnsson A M, Garica F V. Size of the soil microbial biomass in a long-term experiment as affected by different N-fertilizers and organic manures. Soil Biology and Biochemistry, 1993, 25(6): 659-669.

[23] 陈安磊, 王凯荣, 谢小立. 施肥制度与养分循环对稻田土壤微生物生物量碳氮磷的影响. 农业环境科学学报, 2005, 24(6): 1094-1099.

Chen A L, Wang K R, Xie X L. Effects of fertilization systems and nutrient recycling on microbial biomass C, N and P in a red-dish paddy soil. Journal of Agro-Environment Science, 2005, 24(6): 1094-1099. (in Chinese)

[24] Masto R E, Chhonkar P K, Singh D, PATRA A K. Changes in soil biological and biochemical characteristics in a long-term field trial on a subtropical in ceptisol. Soil Biology and Biochemistry, 2006, 38(7): 1577-1582.

[25] Omay A B, Rice C W, Maddux L D, Gordon W B. Changes in soil microbial and chemical properties under long-term crop rotation and fertilization. Soil Science Society of America Journal, 1997, 61(6): 1672-1678．

[26] Jenkinson D S. The effects of biocidal treatments on metabolism in soil-IV. The decomposition of fumigated organisms in soil. Soil Biology and Biochemistry, 1976, 8(3): 203-208．

[27] 周建斌, 陈竹君, 李生秀. 土壤微生物量氮含量、矿化特性及其供氮作用. 生态学报, 2001, 21(10): 1718-1725.

Zhou J B, Chen Z J, Li S X. Contents of soil microbial biomass nitrogen and its mineralized characteristics and relationships with nitrogen supplying ability of soils. Acta Ecologica Sinica, 2001, 21(10): 1718-1725. (in Chinese)

[28] Bremner J M, Jenkinson D S. A rapid method for the determination of organic carbon in soil. Analytica Chimica Acta, 1960, 22(1): 120-124.

[29] 张旭东, 吴刚, 蔡庆华, 彭镇华. 长江干流季节性水淹滩地杨树生长规律研究. 生物数学学报, 1999, 14(3): 322-326

Zhang X D, Wu G, Cai Q H, Peng Z H. A study of the growth rule of poplar on the seasonal river beaches of Changjiang River. Journal of Biomathematics, 1999, 14(3): 322-326. (in Chinese)

[30] CAI A D, FENG W T, ZHANG W J, XU M G. Climate, soil texture, and soil types affect the contributions of fine fraction-stabilized carbon to total soil organic carbon in different land uses across China. Journal of Environmental Management, 2016, 172: 2-9.

[31] WEBB R A. Use of boundary line in analysis of biological data. Horticultural Science, 1972, 47: 309-320.

[32] ANDERSON J, DOMASH K H. Quantities of plant nutrients in the microbial biomass of selected soils. Soil Science, 1980, 130(4): 211-216.

[33] Manjaiah K, Dhyan S. Soil organic matter and biological properties after 26 years of maize-wheat-cowpea cropping as affected by manure and fertilization in a Cambisol in semiarid region of India. Agriculture, Ecosystems and Environment, 2001, 86(2): 155-162.

[34] Goyal S, Chander K, Mundra M C, Kapoor K K. Influence of inorganic fertilizers and organic amendments on soil organic matter and soil microbial properties under tropical conditions. Biology and Fertility of Soils, 1999, 29(2): 196-220.

[35] Sparling G P. Ratio of microbial biomass carbon to soil organic matter carbon as a sensitive indicator of changes in soil organic matter. Australian Journal of Soil Research, 1992, 30(2): 195-207.

[36] Azam F, Yousaf M, Hussain F, Malik K A. Determination of biomass N in some agricultural soils of Punjab, Pakistan. Plant and Soil, 1989, 113(2): 223-228.

[37] 贾伟, 周怀平, 解文艳, 关春林, 郜春花, 石彦琴. 长期有机无机肥配施对褐土微生物生物量碳、氮及酶活性的影响. 植物营养与肥料学报, 2008, 14(4): 700-705.

Jia W, Zhou H P, Jie W Y, Guan C L, Gao C H, Shi Y Q. Effects of long-term inorganic fertilizer combined with organic manure on microbial biomass C, N and enzyme activity in cinnamon soil. Plant Nutrition and Fertilizer Science, 2008, 14(4): 700-705. (in Chinese)

[38] 魏巍, 许艳丽, 朱琳, 韩晓增. 长期施肥对黑土农田土壤微生物群落的影响. 土壤学报, 2013, 50(2): 372-380.

Wei W, Xu Y L, Zhu L, Han X Z. Effect of long-term fertilization on soil microbial communities in farmland of black soil. Acta Pedologica Sinica, 2013, 50(2): 372-380. (in Chinese)

[39] Hopkins D W, Shiel R S. Size and activity of soil microbial communities in long-term experimental grassland plots treated with manure and inorganic fertilizers. Biology and Fertility Soils, 1996, 22(1/2): 66-70.

[40] Bittman S, Forge T A, Kowalenko C G. Responses of the bacterial and fungal biomass in a grassland soil to multi-year applications of dairy manure slurry and fertilizer. Soil Biology and Biochemistry, 2005, 37(4): 613-623.

[41] 韩晓日, 郑国砥, 刘晓燕, 孙振涛, 杨劲峰, 战秀梅. 有机肥与化肥配合施用土壤微生物量氮动态、来源和供氮特征. 中国农业科学, 2007, 40(4): 765-772.

Han X R, Zheng G D, Liu X Y, Sun Z T, Yang J F, Zhan X M. Dynamics, sources and supply charecteristic of microbial biomass nitrogen in soil applied with manure and fertilizer. Scientia Agricultura Sinica, 2007, 40(4): 765-772. (in Chinese)

[42] 胡诚, 曹志平, 胡婵娟, 王金凯. 不同施肥管理措施对土壤碳含量及基础呼吸的影响. 中国生态农业学报, 2007, 15(5): 63-66.

Hu C, Cao Z P, Hu C J, Wang J K. The effects of long-term fertilization on the labile organic carbon and carbon management index in black soil. Chinese Journal of Soil Science, 2007, 15(5): 63-66. (in Chinese)