|
|
|
|
|
| Stability of PAC (Psy-2A-CrtI) gene and agronomic traits in the F2:3 of IR36/PAC transgenic plants |
| Hyun Suk Lee, Gihwan Yi, Kyung Min Kim |
1、Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu 702-701, Republic of Korea
2、Department of Farm Management, College of Agriculture and Life Science, Kyungpook National University, Gunwi-gun 716-821,Republic of Korea |
|
|
|
摘要 This study was carried out to evaluate the expression of yellow endosperm color in the progeny of Psy-2A-CrtI (β-carotene gene, PAC) transgenic plants crossed with IR36. The selected 25 lines of F2 progeny exhibited stable expression of yellow endosperm color and high agronomic characteristics. IR36 and PAC transgenic plant of the grain length, width and ratio showed the same tendency of normal distribution. For progeny selection, colorimeters were as employed to distinguish differing visible colors. Correlation analysis was conducted to investigate the relationship between b* value and the expression of specific genes. b* value was associated with the relative expression of the PAC-Rev and MAR product by real-time PCR and t-test revealed significant difference. Based on this study, correlation between colorimetric values and real-time PCR was effective to detect gene expression. Yellow endosperm progenies represent a new genetic pool that might be useful to increase the genetic diversity of yellow endosperm rice.
Abstract This study was carried out to evaluate the expression of yellow endosperm color in the progeny of Psy-2A-CrtI (β-carotene gene, PAC) transgenic plants crossed with IR36. The selected 25 lines of F2 progeny exhibited stable expression of yellow endosperm color and high agronomic characteristics. IR36 and PAC transgenic plant of the grain length, width and ratio showed the same tendency of normal distribution. For progeny selection, colorimeters were as employed to distinguish differing visible colors. Correlation analysis was conducted to investigate the relationship between b* value and the expression of specific genes. b* value was associated with the relative expression of the PAC-Rev and MAR product by real-time PCR and t-test revealed significant difference. Based on this study, correlation between colorimetric values and real-time PCR was effective to detect gene expression. Yellow endosperm progenies represent a new genetic pool that might be useful to increase the genetic diversity of yellow endosperm rice.
|
|
Received: 27 May 2014
Accepted:
|
| Fund: This work was carried out with the support of the Cooperative Research Program for Agriculture & Technology Development, Rural Development Administration, Republic of Korea (PJ011257012015). |
|
Corresponding Authors:
Kyung Min Kim, Tel: +82-53-9505711, Fax: +82-53-9586880, E-mail: kkm@knu.ac.kr
|
Cite this article:
Hyun Suk Lee, Gihwan Yi, Kyung Min Kim.
2015.
Stability of PAC (Psy-2A-CrtI) gene and agronomic traits in the F2:3 of IR36/PAC transgenic plants. Journal of Integrative Agriculture, 14(6): 1163-1170.
|
| Ameny M A, Wilson P W. 1997. Relationship between Huntercolor values and β-carotene (r=0.74) in white-fleshed Africansweetpotatoes (Ipomoea batatas Lam.). Journal of theScience of Food and Agriculture, 73, 301-306Balestre M, Santos V B, Soares A A, Reis M S. 2010. Stabilityand adaptability of upland rice genotypes. Crop Breedingand Applied Biotechnology, 10, 357-363Beyer P, Salim A B, Ye X, Lucca P, Schaub P, Welsch R,Potrykus I. 2002. Golden rice: Introducing the β-carotenebiosynthesis pathway into rice endosperm by geneticengineering to defeat vitamin A deficiency. Journal Nutrition,132, 506-510Chaudhary N, Nijhawan A, Khurana J P, Khurana P. 2010.Carotene biosynthesis genes in rice: Structural analysis,genome-wide expression profiling and phylogeneticanalysis. Molecular Genetics Genomics, 283, 13-33Cui H, Wang Z, Shu Q, Wu D, Xia Y, Gao M. 2001. Agronomictraits of hybrid progenies between Bt transgenic riceand conventional rice varieties. Chinese Journal of RiceScience, 15, 101-106Gillespie S, Haddad L. 2001. Attacking the Double Burden ofMalnutrition in Asia and the Pacific. Asian DevelopmentBank. p. 10.Ha S H, Kim J B, Park J S, Lee S W, Cho K J. 2007. Acomparison of the carotene accumulation in Capsicumvarieties that show different ripening colours: Deletion of thecapsanthin-capsorubin synthase gene is not a prerequisitefor the formation of a yellow pepper. Journal of ExperimentalBotany, 58, 3135-3144Ha S H, Liang Y S, Jung H R, Ahn M J, Suh S C, Kweon SJ, Kim D H, Kim Y M, Kim J K. 2010. Application of twobicistronic systems involving 2A and IRES sequences tothe biosynthesis of carotenes in rice endosperm. PlantBiotechnology Journal, 8, 928-938Hirschberg J. 2001. Carotenoid biosynthesis in flowering plants.Current Opinion Plant Biology, 4, 210-218Hoa T T C, Al-Babili S, Schaub P, Potrykus I, Beyer P.2003. Golden indica and japonica rice lines amenable toderegulation. Plant Physiology, 133, 161-169Juliano B O. 2003. Rice Chemistry and Quality. 3rd ed.Philippine Rice Research Institute, Manila. pp. 25-39Maoka T, Mochida K, Kozuka M. 2001. Cancer chemopreventiveactivity of carotenes in the fruits of red paprika Capsicum annuum L. Cancer Letters, 172, 103-109Rachel A I, Eileen A K. 2009. Correlation between L*a*b* colorspace values and carotene content in pumpkins and squash(Cucurbita spp.). Hortscience, 44, 633-637Sakamoto T, Matsuoka M. 2006. Characterization ofCONSTITUTIVE PHOTOMORPHOGENESIS ANDDWARFISM homologs in rice (Oryza sativa L.). Journal ofPlant Growth Regulation, 25, 245-251Shah D M, Tumer N E, Fischhoff D A, Horsch R B, Rogers SG, Fraley R T, Jaworski E G. 2013. In: Shah D M, TumerN E, Fischoff D A, Horsch R B, Rogers S G, Fraley R T,Jaworski E G, eds., The Introduction and Expression ofForeign Genes in Plants. The Introduction and Expressionof Foreign Genes in Plants, Biotechnology and GeneticEngineering, London, UK. pp. 81-106Seroczynska A, Korzeniewska A, Wisniewska J S, Szczytt K N,Gajewski M. 2006. Relationship between carotenoid contentand flower or fruit flesh colour of winter squash (Cucurbitamaxima Duch.). Horticulturae, 18, 51-61Simonne A H, Kays S J, Koehler P E, Eitenmiller R R. 1993.Assessment of beta-carotene content in sweet potato(Ipomoea batatas Lam.) breeding lines in relation to dietaryrequirements. Journal of Food Composition and Analysis,6, 336-345Tang G, Hu Y, Yin S A, Wang Y, Dallal G E, Grusak M A,Russell R M. 2012. β-Carotene in Golden Rice is as good asβ-carotene in oil at providing vitamin A to children. AmericanJournal of Clinical Nutrition, 96, 658-664Wang Z, Yu C, Jiang L. 2012. Segregation and expression oftransgenes in the progenies of Bt transgenic rice crossed toconventional rice varieties. African Journal of Biotechnology,11, 7812-7818Yang P, Song M H, Ha S H, Kim J K, Park J S, Ahn S N. 2011.Marker assisted development and characterization ofbeta-carotene rice. Korean Journal of Breeding Science,43, 360-367Ye X, Al-Babili S, Klöti A, Zhang J, Lucca P, Beyer P, PotrykusI. 2000. Engineering the provitamin A (β-carotene)biosynthetic pathway into (carotene-free) rice endosperm.Science, 5, 303-305 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
